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Journals / Turkish Journal of Biology / 2006 / Cilt: 30 - Sayı: 3

Non-Histone Proteins and the Mediator Complex are Essential for the Transcription of HXT2 and HXT4 Genes in Saccharomyces cerevisiae

Sinem GÜZELVARDAR
Sezai TÜRKEL
Pages
163–169
Publication Date
2006-03-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In this study, it was found that the transcription of HXT2 and HXT4 genes decreased 4- to 7-fold in the yeast strain that does not contain the non-histone proteins 6A and 6B (Nhp6A/B).

📌 3 Key Takeaways

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Objective & Hypothesis:Transcriptional activation of HXT2 and HXT4 genes require rapid reorganization of the chromatin structure on the promoter regions of these genes.
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Key Findings & Data:In this study, it was found that the transcription of HXT2 and HXT4 genes decreased 4- to 7-fold in the yeast strain that does not contain the non-histone proteins 6A and 6B (Nhp6A/B).
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Conclusion & Impact:These results indicated that Nhp6A/B and the mediator complex are essential for the regulation of HXT2 and HXT4 gene transcription in S. cerevisiae.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Saccharomyces cerevisiae · glucose repression · hexose transporters

Abstract

Transcriptional activation of HXT2 and HXT4 genes require rapid reorganization of the chromatin structure on the promoter regions of these genes. In this study, it was found that the transcription of HXT2 and HXT4 genes decreased 4- to 7-fold in the yeast strain that does not contain the non-histone proteins 6A and 6B (Nhp6A/B). In addition, results of this research indicated that Nhp6A/B are required for the rapid derepression and activation of HXT2 and HXT4 transcription upon glucose signaling. Furthermore, it was found that Med2p, which provides the connection between the transcriptional activators and the basal transcription factors, is also required for the activation of HXT2 and HXT4 transcription. Transcription of HXT2 and HXT4 decreased 2- to 13-fold in Med2 mutant S. cerevisiae cells. These results indicated that Nhp6A/B and the mediator complex are essential for the regulation of HXT2 and HXT4 gene transcription in S. cerevisiae.

Özet

Transcriptional activation of HXT2 and HXT4 genes require rapid reorganization of the chromatin structure on the promoter regions of these genes. In this study, it was found that the transcription of HXT2 and HXT4 genes decreased 4- to 7-fold in the yeast strain that does not contain the non-histone proteins 6A and 6B (Nhp6A/B). In addition, results of this research indicated that Nhp6A/B are required for the rapid derepression and activation of HXT2 and HXT4 transcription upon glucose signaling. Furthermore, it was found that Med2p, which provides the connection between the transcriptional activators and the basal transcription factors, is also required for the activation of HXT2 and HXT4 transcription. Transcription of HXT2 and HXT4 decreased 2- to 13-fold in Med2 mutant S. cerevisiae cells. These results indicated that Nhp6A/B and the mediator complex are essential for the regulation of HXT2 and HXT4 gene transcription in S. cerevisiae.

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Cite This Article

GÜZELVARDAR, S., TÜRKEL, S. (2006). Non-Histone Proteins and the Mediator Complex are Essential for the Transcription of HXT2 and HXT4 Genes in Saccharomyces cerevisiae. Turkish Journal of Biology, Cilt: 30 - Sayı: 3, 163–169. https://ajindex.com/articles/182249

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