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Journals / Turkish Journal of Biology / 2009 / Cilt: 33 - Sayı: 1

Identification of Lactococcus lactis subsp. Isolates Based on Their Cell Wall Protein Profiles and Plasmid Contents

Mehtap ÜSTÜN
Banu ÖZDEN
Ömer ŞİMŞEK
Mustafa AKÇELİK
Pages
21–27
Publication Date
2009-01-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Also a key plasmid profile, which would be used to determine the isolates at subspecies level, could not be obtained.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, among the total 50 lactococci isolates, 44% L. lactis subsp. lactis, 28% L. lactis subsp. lactis biovar. diacetylactis and 28% L. lactis subsp. cremoris strains were identified by their phenotypic characterization.
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Key Findings & Data:According to the cell wall protein patterns, 8.6, 12.5, 14.7, 20.3, 23.4, 25.4, and 31.8 kDa proteins were found identical within 82% of isolates.
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Conclusion & Impact:Also a key plasmid profile, which would be used to determine the isolates at subspecies level, could not be obtained.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:L. lactis · cell wall proteins · plasmid

Abstract

In this study, among the total 50 lactococci isolates, 44% L. lactis subsp. lactis, 28% L. lactis subsp. lactis biovar. diacetylactis and 28% L. lactis subsp. cremoris strains were identified by their phenotypic characterization. According to the cell wall protein patterns, 8.6, 12.5, 14.7, 20.3, 23.4, 25.4, and 31.8 kDa proteins were found identical within 82% of isolates. Moreover 93% of L. lactis subsp. cremoris strains contained a unique 157.8 kDa protein; however, L. lactis subsp. lactis and L. lactis subsp. lactis biovar. diacetylactis had no unique protein in their groups. Also a key plasmid profile, which would be used to determine the isolates at subspecies level, could not be obtained.

Özet

In this study, among the total 50 lactococci isolates, 44% L. lactis subsp. lactis, 28% L. lactis subsp. lactis biovar. diacetylactis and 28% L. lactis subsp. cremoris strains were identified by their phenotypic characterization. According to the cell wall protein patterns, 8.6, 12.5, 14.7, 20.3, 23.4, 25.4, and 31.8 kDa proteins were found identical within 82% of isolates. Moreover 93% of L. lactis subsp. cremoris strains contained a unique 157.8 kDa protein; however, L. lactis subsp. lactis and L. lactis subsp. lactis biovar. diacetylactis had no unique protein in their groups. Also a key plasmid profile, which would be used to determine the isolates at subspecies level, could not be obtained.

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Cite This Article

ÜSTÜN, M., ÖZDEN, B., ŞİMŞEK, Ö., AKÇELİK, M. (2009). Identification of Lactococcus lactis subsp. Isolates Based on Their Cell Wall Protein Profiles and Plasmid Contents. Turkish Journal of Biology, Cilt: 33 - Sayı: 1, 21–27. https://ajindex.com/articles/182226

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References (12)

  1. Huggins AR. Progress in dairy starter culture technology. Food Technology 38: 41-50, 1984. 2. Nomura M, Kimoto H, Someya Y et al. Novel characteristic for distinguishing Lactococcus lactis subsp. lactis from subsp. cremoris. International J Systematic Bacteriology 49: 163-166, 1999. 3. Perez G, Cardell E, Zarate V. Protein fingerprinting as a complementary analysis to classical phenotyping for the Allison GE, Klaenhammer TR. Phage resistance mechanisms in lactic acid bacteria. Int Dairy Journal 8: 207-226, 1998. Akçelik M. Plasmid mediated industrial traits in Lactococcus lactis subsp.lactis LL140. Milchwissenschaft 54: 603-606, 1999. Akçelik M. Identification of lactose utilization and copper resistance plasmid Lactococcus lactis subsp. lactis MCL64. Turk J Vet Anim Sci 25: 783-787, 2001.
  2. Boucher I, Emond E, Parrot M et al. DNA squence analysis of three Lactococcus lactis mechanisms. J.Dairy Sci 84: 1610-1620, 2001.
  3. Moineau S, Tremnlay D, Labrie S. Phages of lactic acid bacteria. From Genomics to Instrial Applications AMS News. 388-393, 2002.
  4. Siezen RJ, Enckevort FH, Kalenhammer M et al. Genome data mining of lactic acid bacteria; impact of bioinformation. Curr Opin Biotechnol 15: 105-115, 2004.
  5. Bellocine K, Yam KK, Causineau B. Conjugative transfer of the Lactococcus lactis chromosomal sex factor promotes dissemination of the group II intron. J Bacteriol 187: 930-939, 2005.
  6. Reddy MS, Vedamuthu ER, Washam CJ et al. Different agar medium for separating Streptococcus lactis and Streptococcus cremoris. Appl Microbiol 18: 755-759, 1969.
  7. Kempler GM, McKay LL. Improved medium for detection of citrate- fermenting Streptococcus lactis subsp. diacetylactis. Appl Environ Microbiol 39: 926-927, 1980.
  8. Piard JC, Hautefort I, Fischetti VA et al. Cell wall anchoring of the Streptococcus pyogenes M6 protein in various lactic acid bacteria. J Bacteriol 179: 3068-3072, 1997.
  9. Anderson DG, McKay LL. A simple and rapid metod for isolating large plasmid DNA from lactic streptococci. Appl Environ Microbiol 46: 549-552, 1983.
  10. Ward LJ, Brown JC, Davey GP. Two methods for the genetic differentiation of Lactococcus lactis subsp. lactis and cremoris based on differences in the 16S rRNA gene squence. FEMS Microbiology Letters. 166: 15-20, 1998.
  11. Frees D, Vogensen FK, Ingmer H. Identification of proteins induced at low pH in Lactococcus lactis. Int J Food Microbiol 87: 293-300, 2003.
  12. Sijstma L, Wouters JTM, Hellingwerf KJ. Isolation and characterization of lipoteichoic acid, a cell env