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Journals / Turkish Journal of Biology / 2011 / Cilt: 35 - Sayı: 3

An entomopathogenic bacterium, Pseudomonas putida, from Leptinotarsa decemlineata

Hacer MURATOĞLU
Zihni DEMİRBAĞ
Kazım SEZEN
Pages
275–282
Publication Date
2011-03-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Our results indicate that Ld4 isolate may be valuable as a biological control agent for the biological control of L. decemlineata.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The Colorado Potato Beetle (CPB), Leptinotarsa decemlineata (Say) (Col.: Chrysomelidae), is a pre-eminent pest on solanaceous crops worldwide, and much research has focused on the different components of its variable population dynamics.
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Key Findings & Data:In this study, in order to find a more effective and safer biological control agent against L. decemlineata, we isolated a new bacterial isolate (Ld4) from L. decemlineata, and tested it for insecticidal effects on L. decemlineata larvae.
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Conclusion & Impact:Our results indicate that Ld4 isolate may be valuable as a biological control agent for the biological control of L. decemlineata.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Key words: Pseudomonas putida · insecticidal activity · microbial control

Abstract

The Colorado Potato Beetle (CPB), Leptinotarsa decemlineata (Say) (Col.: Chrysomelidae), is a pre-eminent pest on solanaceous crops worldwide, and much research has focused on the different components of its variable population dynamics. L. decemlineata has developed resistance to insecticides used for its control. Consequently, alternative methods are needed urgently for the control of this pest. In this study, in order to find a more effective and safer biological control agent against L. decemlineata, we isolated a new bacterial isolate (Ld4) from L. decemlineata, and tested it for insecticidal effects on L. decemlineata larvae. Also, the insecticidal effect of Ld4 was compared with Ld3, which was isolated from L. decemlineata. According to the morphological, physiological, biochemical tests, API20E and API50CH panel test systems, and 16S rRNA sequence homologies, this isolate was identified as Pseudomonas putida. The highest insecticidal effect determined on L. decemlineata within 5 days was 100%, and this activity was exhibited by Ld4 isolate. Our results indicate that Ld4 isolate may be valuable as a biological control agent for the biological control of L. decemlineata.

Özet

The Colorado Potato Beetle (CPB), Leptinotarsa decemlineata (Say) (Col.: Chrysomelidae), is a pre-eminent pest on solanaceous crops worldwide, and much research has focused on the different components of its variable population dynamics. L. decemlineata has developed resistance to insecticides used for its control. Consequently, alternative methods are needed urgently for the control of this pest. In this study, in order to find a more effective and safer biological control agent against L. decemlineata, we isolated a new bacterial isolate (Ld4) from L. decemlineata, and tested it for insecticidal effects on L. decemlineata larvae. Also, the insecticidal effect of Ld4 was compared with Ld3, which was isolated from L. decemlineata. According to the morphological, physiological, biochemical tests, API20E and API50CH panel test systems, and 16S rRNA sequence homologies, this isolate was identified as Pseudomonas putida. The highest insecticidal effect determined on L. decemlineata within 5 days was 100%, and this activity was exhibited by Ld4 isolate. Our results indicate that Ld4 isolate may be valuable as a biological control agent for the biological control of L. decemlineata.

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Cite This Article

MURATOĞLU, H., DEMİRBAĞ, Z., SEZEN, K. (2011). An entomopathogenic bacterium, Pseudomonas putida, from Leptinotarsa decemlineata. Turkish Journal of Biology, Cilt: 35 - Sayı: 3, 275–282. https://ajindex.com/articles/182013

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