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Journals / Turkish Journal of Biology / 2011 / Cilt: 35 - Sayı: 3

Molecular diversity of thermophilic bacteria isolated from Pasinler hot spring (Erzurum, Turkey)

Ahmet ADIGÜZEL
Kadriye İNAN
Fikrettin ŞAHİN
Tulin ARASOĞLU
Medine GÜLLÜCE
Pages
267–274
Publication Date
2011-03-01
DOI
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⚡ TL;DR(Too Long; Didn't Read)

The first group, identified as Bacillus licheniformis, was represented by 4 strains, and the second group, identified as Aeribacillus pallidus, was represented by 5 strains.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The present study was conducted to determine the phenotypic and genotypic characterization of thermophilic bacteria isolated from Pasinler hot spring, Erzurum, Turkey.
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Key Findings & Data:Fatty acid profiles, BOX PCR fingerprints, and 16S rDNA sequence data were used for the phenotypic and genotypic characterization of thermophilic bacteria.
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Conclusion & Impact:The first group, identified as Bacillus licheniformis, was represented by 4 strains, and the second group, identified as Aeribacillus pallidus, was represented by 5 strains.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Key words: Thermophile bacteria · FAMEs · BOX-PCR

Abstract

The present study was conducted to determine the phenotypic and genotypic characterization of thermophilic bacteria isolated from Pasinler hot spring, Erzurum, Turkey. Fatty acid profiles, BOX PCR fingerprints, and 16S rDNA sequence data were used for the phenotypic and genotypic characterization of thermophilic bacteria. Totally 9 different bacterial strains were selected based on morphological, physiological, and biochemical tests. These strains were characterized by molecular tests including fatty acid and BOX profiles, and 16S rDNA sequence. The data of fatty acid analysis showed the presence of 14 different fatty acids in the 9 bacterial strains examined. Additionally, 5 of these fatty acids, 15:0 iso, 15:0 anteiso, 16:0 iso, 17:0 iso, and 17:0 anteiso fatty acids, were found in all isolates. Based on fatty acid profiles, it was determined that the bacterial strains were classified into 2 phenotypic groups, and these data were confirmed by BOX PCR genomic fingerprint profiles and 16S rDNA sequence analysis. The first group, identified as Bacillus licheniformis, was represented by 4 strains, and the second group, identified as Aeribacillus pallidus, was represented by 5 strains.

Özet

The present study was conducted to determine the phenotypic and genotypic characterization of thermophilic bacteria isolated from Pasinler hot spring, Erzurum, Turkey. Fatty acid profiles, BOX PCR fingerprints, and 16S rDNA sequence data were used for the phenotypic and genotypic characterization of thermophilic bacteria. Totally 9 different bacterial strains were selected based on morphological, physiological, and biochemical tests. These strains were characterized by molecular tests including fatty acid and BOX profiles, and 16S rDNA sequence. The data of fatty acid analysis showed the presence of 14 different fatty acids in the 9 bacterial strains examined. Additionally, 5 of these fatty acids, 15:0 iso, 15:0 anteiso, 16:0 iso, 17:0 iso, and 17:0 anteiso fatty acids, were found in all isolates. Based on fatty acid profiles, it was determined that the bacterial strains were classified into 2 phenotypic groups, and these data were confirmed by BOX PCR genomic fingerprint profiles and 16S rDNA sequence analysis. The first group, identified as Bacillus licheniformis, was represented by 4 strains, and the second group, identified as Aeribacillus pallidus, was represented by 5 strains.

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Cite This Article

ADIGÜZEL, A., İNAN, K., ŞAHİN, F., ARASOĞLU, T., GÜLLÜCE, M. (2011). Molecular diversity of thermophilic bacteria isolated from Pasinler hot spring (Erzurum, Turkey). Turkish Journal of Biology, Cilt: 35 - Sayı: 3, 267–274. https://ajindex.com/articles/182005

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