Journals / Turkish Journal of Biology / 2000 / Cilt: 24 - Sayı: 3

The Effects of Different Carbon Sources and C/N Ratio on Microbial Denitrification

Pages
533–542
Publication Date
2000-03-01
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

On the other hand, 29% NO 3 reduction (C/N = 0,5) for P. denitrificans and 59% NO 3 reduction (C/N = 1) for P. stutzeri were obtained when acetic acid was used as a carbon source.

📌 3 Key Takeaways

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Objective & Hypothesis:The effects of different carbon sources (ethanol, methanol and acetic acid) and C/N ratios (0,5; 1; 1,5; 2; 2,5) on the microbial denitrification in batch culture were investigated.
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Key Findings & Data:For all experiments, the initial NO 3 concentrations were constant at 100 mg/l.
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Conclusion & Impact:On the other hand, 29% NO 3 reduction (C/N = 0,5) for P. denitrificans and 59% NO 3 reduction (C/N = 1) for P. stutzeri were obtained when acetic acid was used as a carbon source.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Batch culture · denitrification · Pseudomonas stutzeri

Abstract

The effects of different carbon sources (ethanol, methanol and acetic acid) and C/N ratios (0,5; 1; 1,5; 2; 2,5) on the microbial denitrification in batch culture were investigated. For this purpose Paracoccus denitrificans and Pseudomonas stutzeri were used as denitrifiers. For all experiments, the initial NO 3 concentrations were constant at 100 mg/l. During the incubation period of 66 hours, nitrate, nitrite and microorganism concentrations were determined spectrophotometrically at certain intervals. When ethanol was used as a carbon source, the highest NO 3 reductions for P. denitrificans and P. stutzeriwere found to be 40% (C/N = 1) and 32% (C/N = 2,5), respectively. When methanol was used as a carbon source, the highest NO 3 reductions for these two species were 35% (C/N = 0,5) and 20% (C/N = 1), respectively. On the other hand, 29% NO 3 reduction (C/N = 0,5) for P. denitrificans and 59% NO 3 reduction (C/N = 1) for P. stutzeri were obtained when acetic acid was used as a carbon source.

Özet

The effects of different carbon sources (ethanol, methanol and acetic acid) and C/N ratios (0,5; 1; 1,5; 2; 2,5) on the microbial denitrification in batch culture were investigated. For this purpose Paracoccus denitrificans and Pseudomonas stutzeri were used as denitrifiers. For all experiments, the initial NO 3 concentrations were constant at 100 mg/l. During the incubation period of 66 hours, nitrate, nitrite and microorganism concentrations were determined spectrophotometrically at certain intervals. When ethanol was used as a carbon source, the highest NO 3 reductions for P. denitrificans and P. stutzeriwere found to be 40% (C/N = 1) and 32% (C/N = 2,5), respectively. When methanol was used as a carbon source, the highest NO 3 reductions for these two species were 35% (C/N = 0,5) and 20% (C/N = 1), respectively. On the other hand, 29% NO 3 reduction (C/N = 0,5) for P. denitrificans and 59% NO 3 reduction (C/N = 1) for P. stutzeri were obtained when acetic acid was used as a carbon source.

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Cite This Article

ÖKMEN, G. (. (2000). The Effects of Different Carbon Sources and C/N Ratio on Microbial Denitrification. Turkish Journal of Biology, Cilt: 24 - Sayı: 3, 533–542. https://ajindex.com/articles/181942

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