Journals / Turkish Journal of Biology / 2000 / Cilt: 24 - Sayı: Ek

In Vitro Propagation of Thymus sipyleus Boiss. (Lamiaceae)

Pages
81–86
Publication Date
1999-12-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Plantlets which regenerated on the same medium formed multiple shoots within 2 weeks and they were rooted after the 4th subculture.

📌 3 Key Takeaways

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Objective & Hypothesis:In vitro propagation of Thymus sipyleus Boiss. (Lamiaceae), an endemic from western Anatolia was investigated.
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Key Findings & Data:Then, seedlings were transferred to Murashige-Skoog basal medium containing 0.4 mg/l Naphtaleneacetic acid, (NAA) and 3 mg/l Benzyladenin (BA) and then they formed callus where they were in contact with the medium.
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Conclusion & Impact:Plantlets which regenerated on the same medium formed multiple shoots within 2 weeks and they were rooted after the 4th subculture.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Thymus sipyleus · callus formation · plant regeneration

Abstract

In vitro propagation of Thymus sipyleus Boiss. (Lamiaceae), an endemic from western Anatolia was investigated. Sterilized seeds of the plant were germinated on modified Murashige-Skoog medium (mMS) and Heller's nutrient media. Then, seedlings were transferred to Murashige-Skoog basal medium containing 0.4 mg/l Naphtaleneacetic acid, (NAA) and 3 mg/l Benzyladenin (BA) and then they formed callus where they were in contact with the medium. Plantlets which regenerated on the same medium formed multiple shoots within 2 weeks and they were rooted after the 4th subculture.

Özet

In vitro propagation of Thymus sipyleus Boiss. (Lamiaceae), an endemic from western Anatolia was investigated. Sterilized seeds of the plant were germinated on modified Murashige-Skoog medium (mMS) and Heller's nutrient media. Then, seedlings were transferred to Murashige-Skoog basal medium containing 0.4 mg/l Naphtaleneacetic acid, (NAA) and 3 mg/l Benzyladenin (BA) and then they formed callus where they were in contact with the medium. Plantlets which regenerated on the same medium formed multiple shoots within 2 weeks and they were rooted after the 4th subculture.

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Cite This Article

ERDAĞ, B. B. (1999). In Vitro Propagation of Thymus sipyleus Boiss. (Lamiaceae). Turkish Journal of Biology, Cilt: 24 - Sayı: Ek, 81–86. https://ajindex.com/articles/181866

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