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Journals / Turkish Journal of Biology / 2013 / Cilt: 37 - Sayı: 4

Assessment of genetic diversity in naturally growing 29 Trifolium L. taxa from Bolu Province using RAPD and SSR markers

Emel USLU
Gözde Damla ERTUĞRUL
Mehmet Tekin BABAÇ
Pages
479–490
Publication Date
2013-04-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results suggest that the use of 2 different molecular markers gives the most reliable genetic data for Trifolium taxa.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Two molecular marker technologies, RAPD and SSR, were used to determine the genetic diversity of 29 taxa of Trifolium L. collected from Bolu Province.
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Key Findings & Data:The analysis was carried out using 11 RAPD and 5 SSR markers.
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Conclusion & Impact:The results suggest that the use of 2 different molecular markers gives the most reliable genetic data for Trifolium taxa.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Key words: Trifolium · Fabaceae · Bolu

Abstract

Two molecular marker technologies, RAPD and SSR, were used to determine the genetic diversity of 29 taxa of Trifolium L. collected from Bolu Province. The analysis was carried out using 11 RAPD and 5 SSR markers. A total of 446 and 331 fragments were produced by RAPD and SSR markers, respectively. The amplification products were 300-3500 bp with RAPD and 125-3000 bp with SSR markers. These bands were scored as absence and presence for all taxa. Data obtained were used to estimate genetic similarity using Jaccard's coefficient, and dendrograms were constructed by the UPGMA method using either RAPD and SSR markers individually or as combined sets of data. The highest genetic similarity values obtained were 0.33 between T. hirtum All. and T. constantinapolitanum Ser. for RAPD and 0.49 between T. medium L. var. medium and var. eriocalycinum Hausskn. for SSR. The dendrograms produced from combined sets of RAPD and SSR data revealed 2 main clusters. One contained members of the sections Chronosemium Ser., Lotoidea L., and Vesicaria Ser., and the other had only the section Trifolium. The results suggest that the use of 2 different molecular markers gives the most reliable genetic data for Trifolium taxa.

Özet

Two molecular marker technologies, RAPD and SSR, were used to determine the genetic diversity of 29 taxa of Trifolium L. collected from Bolu Province. The analysis was carried out using 11 RAPD and 5 SSR markers. A total of 446 and 331 fragments were produced by RAPD and SSR markers, respectively. The amplification products were 300-3500 bp with RAPD and 125-3000 bp with SSR markers. These bands were scored as absence and presence for all taxa. Data obtained were used to estimate genetic similarity using Jaccard's coefficient, and dendrograms were constructed by the UPGMA method using either RAPD and SSR markers individually or as combined sets of data. The highest genetic similarity values obtained were 0.33 between T. hirtum All. and T. constantinapolitanum Ser. for RAPD and 0.49 between T. medium L. var. medium and var. eriocalycinum Hausskn. for SSR. The dendrograms produced from combined sets of RAPD and SSR data revealed 2 main clusters. One contained members of the sections Chronosemium Ser., Lotoidea L., and Vesicaria Ser., and the other had only the section Trifolium. The results suggest that the use of 2 different molecular markers gives the most reliable genetic data for Trifolium taxa.

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Cite This Article

USLU, E., ERTUĞRUL, G. D., BABAÇ, M. T. (2013). Assessment of genetic diversity in naturally growing 29 Trifolium L. taxa from Bolu Province using RAPD and SSR markers. Turkish Journal of Biology, Cilt: 37 - Sayı: 4, 479–490. https://ajindex.com/articles/181844

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