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Journals / Turkish Journal of Biology / 2013 / Cilt: 37 - Sayı: 4

Characterization of a new isolate of Malacosoma neustria nucleopolyhedrovirus (ManeNPV) from Turkey

İsmail DEMİR
Remziye NALÇACIOĞLU
Lida MOHAMMAD GHOLİZAD
Zihni DEMİRBAĞ
Pages
385–391
Publication Date
2013-04-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Consequently, ManeNPV appears to be a promising microbial control agent for biocontrol of M. neustria.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The lackey moth, Malacosoma neustria (Linnaeus, 1758), a worldwide pest, causes extensive economic losses on particularly hazelnut, Prunus, Quercus, Populus, and Salix trees.
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Key Findings & Data:It was named ManeNPV-T2.
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Conclusion & Impact:Consequently, ManeNPV appears to be a promising microbial control agent for biocontrol of M. neustria.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Key words: Malacosoma neustria · nucleopolyhedrovirus · characterization

Abstract

The lackey moth, Malacosoma neustria (Linnaeus, 1758), a worldwide pest, causes extensive economic losses on particularly hazelnut, Prunus, Quercus, Populus, and Salix trees. In this study, a local nucleopolyhedrovirus (NPV) was isolated from the larvae of M. neustria in the northeast of Turkey. It was named ManeNPV-T2. Electron microscopic observations showed that the polyhedra of the new isolate contain several virions with multiple nucleocapsids packaged within a single viral envelope. Restriction endonuclease analysis of ManeNPV-T2 DNA indicated that it has a different restriction profile compared to the previous Turkish and Latvian isolates. The phylogenetic analysis of the amplified polh sequence of ManeNPV-T2 showed its relation to the other NPVs from Malacosoma species. Mortality values for third instar M. neustria larvae ranged from 48% to 100% according to the concentration of polyhedral occlusion bodies. Based on screening tests, the LD50 value of the new isolate was calculated as 1.34 × 104 occlusion bodies per larvae. Consequently, ManeNPV appears to be a promising microbial control agent for biocontrol of M. neustria.

Özet

The lackey moth, Malacosoma neustria (Linnaeus, 1758), a worldwide pest, causes extensive economic losses on particularly hazelnut, Prunus, Quercus, Populus, and Salix trees. In this study, a local nucleopolyhedrovirus (NPV) was isolated from the larvae of M. neustria in the northeast of Turkey. It was named ManeNPV-T2. Electron microscopic observations showed that the polyhedra of the new isolate contain several virions with multiple nucleocapsids packaged within a single viral envelope. Restriction endonuclease analysis of ManeNPV-T2 DNA indicated that it has a different restriction profile compared to the previous Turkish and Latvian isolates. The phylogenetic analysis of the amplified polh sequence of ManeNPV-T2 showed its relation to the other NPVs from Malacosoma species. Mortality values for third instar M. neustria larvae ranged from 48% to 100% according to the concentration of polyhedral occlusion bodies. Based on screening tests, the LD50 value of the new isolate was calculated as 1.34 × 104 occlusion bodies per larvae. Consequently, ManeNPV appears to be a promising microbial control agent for biocontrol of M. neustria.

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Cite This Article

DEMİR, İ., NALÇACIOĞLU, R., GHOLİZAD, L. M., DEMİRBAĞ, Z. (2013). Characterization of a new isolate of Malacosoma neustria nucleopolyhedrovirus (ManeNPV) from Turkey. Turkish Journal of Biology, Cilt: 37 - Sayı: 4, 385–391. https://ajindex.com/articles/181832

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