Proteomic evidence for the plasticity of cultured vascular smooth muscle cells

Objective & Hypothesis:Morphological, functional, and gene expression studies have established the phenotypic plasticity of smooth muscle cells (SMCs).

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Key Findings & Data:These cells have been shown to respond to environmental stimulants such as extracellular matrix and growth factors.

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Conclusion & Impact:The individual proteins and their significance are discussed within the scope of this paper.

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Key words: Thoracic aortic aneurysm · label-free LC-MS · smooth muscle cell

Abstract

Morphological, functional, and gene expression studies have established the phenotypic plasticity of smooth muscle cells (SMCs). These cells have been shown to respond to environmental stimulants such as extracellular matrix and growth factors. Cellular changes can vary between extremes defined as the contractile and synthetic states. Various growth factors have been shown to have profound effects on the phenotype of these cells. In this study, we intended to investigate the effects of growth factor-rich medium on the protein expression of vascular SMCs in culture. Interestingly, transiently changing the type of medium did not result in any apparent morphological differences, yet we hypothesized that some cellular factors might still be altered. In order to understand what kind of intracellular molecular changes should be expected to occur during the medium change, we analyzed global protein expression changes using nano-LC-MS/MS in smooth muscle cell cultures that were isolated and grown in one medium formulation and temporarily switched to the other. Our data indicate that proteins playing a role in energy metabolism (glycolysis), translation, and folding of proteins are affected, along with regulatory molecules and cytoskeletal proteins. The individual proteins and their significance are discussed within the scope of this paper.

Özet

Morphological, functional, and gene expression studies have established the phenotypic plasticity of smooth muscle cells (SMCs). These cells have been shown to respond to environmental stimulants such as extracellular matrix and growth factors. Cellular changes can vary between extremes defined as the contractile and synthetic states. Various growth factors have been shown to have profound effects on the phenotype of these cells. In this study, we intended to investigate the effects of growth factor-rich medium on the protein expression of vascular SMCs in culture. Interestingly, transiently changing the type of medium did not result in any apparent morphological differences, yet we hypothesized that some cellular factors might still be altered. In order to understand what kind of intracellular molecular changes should be expected to occur during the medium change, we analyzed global protein expression changes using nano-LC-MS/MS in smooth muscle cell cultures that were isolated and grown in one medium formulation and temporarily switched to the other. Our data indicate that proteins playing a role in energy metabolism (glycolysis), translation, and folding of proteins are affected, along with regulatory molecules and cytoskeletal proteins. The individual proteins and their significance are discussed within the scope of this paper.

Keywords

Key words: Thoracic aortic aneurysm label-free LC-MS smooth muscle cell pathway analysis smooth muscle cell growth medium DMEM/F12 growth medium

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Cite This Article

BAYKAL, A. T., BAYKAL, B., SERHATLI, M., ADIGÜZEL, Z., TUNÇER, M. A., KAÇAR, Ö. (2013). Proteomic evidence for the plasticity of cultured vascular smooth muscle cells. Turkish Journal of Biology, Cilt: 37 - Sayı: 4, 414–425. https://ajindex.com/articles/181830

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References (15)

Eagle H. Nutrition needs of mammalian cells in tissue culture. Science 122: 501–14, 1955.
Amirkia V, Qiubao P. Cell-culture Database: Literature-based reference tool for human and mammalian experimentally based cell culture applications. Bioinformation 8: 237–8, 2012.
Owens GK, Kumar MS, Wamhoff BR. Molecular regulation of vascular smooth muscle cell differentiation in development and disease. Physiol Rev 84: 767–801, 2004.
Beamish JA, He P, Kottke-Marchant K et al. Molecular regulation of contractile smooth muscle cell phenotype: implications for vascular tissue engineering. Tissue Eng Pt B-Rev 16: 467–91, 2010.
Kumar MS, Owens GK. Combinatorial control of smooth muscle-specific gene expression. Arterioscl Throm Vas 23: 737–47, 2003.
Ailawadi G, Moehle CW, Pei H et al. Smooth muscle phenotypic modulation is an early event in aortic aneurysms. J Thorac Cardiovasc Surg 138: 1392–9, 2009.
Chamley-Campbell J, Campbell GR, Ross R. The smooth muscle cell in culture. Physiol Rev 59: 1–61, 1979.
Hayashi K, Saga H, Chimori Y et al. Differentiated phenotype of smooth muscle cells depends on signaling pathways through insulin-like growth factors and phosphatidylinositol 3-kinase. J Biol Chem 273: 28860–7, 1998.
Hedin U, Daum G, Clowes AW. Heparin inhibits thrombininduced mitogen-activated protein kinase signaling in arterial smooth muscle cells. J Vasc Surg 27: 512–20, 1998.
Pukac L, Huangpu J, Karnovsky MJ. Platelet-derived growth factor-BB, insulin-like growth factor-I, and phorbol ester activate different signaling pathways for stimulation of vascular smooth muscle cell migration. Exp Cell Res 242: 548–60, 1998.
Pukac LA, Carter JE, Ottlinger ME et al. Mechanisms of inhibition by heparin of PDGF stimulated MAP kinase activation in vascular smooth muscle cells. J Cell Physiol 172: 69–78, 1997.
Daum G, Hedin U, Wang Y et al. Diverse effects of heparin on mitogen-activated protein kinase-dependent signal transduction in vascular smooth muscle cells. Circ Res 81: 17–23, 1997.
Rauch BH, Millette E, Kenagy RD et al. Thrombin- and factor Xa-induced DNA synthesis is mediated by transactivation of fibroblast growth factor receptor-1 in human vascular smooth muscle cells. Circ Res 94: 340–5, 2004.
Louwrens HD, Kwaan HC, Pearce WH et al. Plasminogen activator and plasminogen activator inhibitor expression by normal and aneurysmal human aortic smooth muscle cells in culture. Eur J Vasc Endovasc 10: 289–93, 1995.
McMillan WD, Patterson BK, Keen RR et al. In situ localization