Journals / Turkish Journal of Biology / 2013 / Cilt: 37 - Sayı: 1

Using seedlessness-related molecular markers in grapevine breeding for seedlessness via marker-assisted selection into Muscat of Hamburg × Sultani progeny

Pages
101–105
Publication Date
2013-01-01
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

A total of 13 individuals that carried alleles for the 3 markers associated with seedlessness were selected as genetic resources for future studies on seedless grapevine breeding.

📌 3 Key Takeaways

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Objective & Hypothesis:The seedlessness-related molecular markers VMC7f2, SCC8, and SCF27 were used for the early selection of seedless individuals in the Muscat of Hamburg (seeded) × Sultani (seedless) progeny of 314 F1 hybrids.
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Key Findings & Data:The DNA from F1 hybrids was amplified by polymerase chain reaction (PCR) using 3 markers.
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Conclusion & Impact:A total of 13 individuals that carried alleles for the 3 markers associated with seedlessness were selected as genetic resources for future studies on seedless grapevine breeding.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Key words: Grapevine · marker-assisted selection · seedlessness

Abstract

The seedlessness-related molecular markers VMC7f2, SCC8, and SCF27 were used for the early selection of seedless individuals in the Muscat of Hamburg (seeded) × Sultani (seedless) progeny of 314 F1 hybrids. The DNA from F1 hybrids was amplified by polymerase chain reaction (PCR) using 3 markers. After digestion of the SCC8 marker PCR amplification products by restriction enzyme Bgl II, 72 individuals showed homozygous SCC8+/SCC8+ alleles at the seed development inhibitor locus, 76 individuals of the progeny amplified with the SCF27 marker produced 2.0-kb bands, and 118 individuals with seedlessness-related 198-bp alleles amplified with the VMC7f2 marker were selected as seedless grapevine candidates. Based on marker-assisted selection, 190 F1 hybrids were designated as seedless grapevine candidates since they carried a minimum of 1 seedlessness-related allele, and the 124 F1 hybrids were removed from the seedless grapevine breeding studies. A total of 13 individuals that carried alleles for the 3 markers associated with seedlessness were selected as genetic resources for future studies on seedless grapevine breeding.

Özet

The seedlessness-related molecular markers VMC7f2, SCC8, and SCF27 were used for the early selection of seedless individuals in the Muscat of Hamburg (seeded) × Sultani (seedless) progeny of 314 F1 hybrids. The DNA from F1 hybrids was amplified by polymerase chain reaction (PCR) using 3 markers. After digestion of the SCC8 marker PCR amplification products by restriction enzyme Bgl II, 72 individuals showed homozygous SCC8+/SCC8+ alleles at the seed development inhibitor locus, 76 individuals of the progeny amplified with the SCF27 marker produced 2.0-kb bands, and 118 individuals with seedlessness-related 198-bp alleles amplified with the VMC7f2 marker were selected as seedless grapevine candidates. Based on marker-assisted selection, 190 F1 hybrids were designated as seedless grapevine candidates since they carried a minimum of 1 seedlessness-related allele, and the 124 F1 hybrids were removed from the seedless grapevine breeding studies. A total of 13 individuals that carried alleles for the 3 markers associated with seedlessness were selected as genetic resources for future studies on seedless grapevine breeding.

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Cite This Article

AKKURT, M., ÇAKIR, A., SHİDFAR, M., MUTAF, F., SÖYLEMEZOĞLU, G. (2013). Using seedlessness-related molecular markers in grapevine breeding for seedlessness via marker-assisted selection into Muscat of Hamburg × Sultani progeny. Turkish Journal of Biology, Cilt: 37 - Sayı: 1, 101–105. https://ajindex.com/articles/181786

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