SavedAdd your journal

Journals / Turkish Journal of Biology / 2013 / Cilt: 37 - Sayı: 1

The effect of 3-methylcholanthrene and butylated hydroxytoluene on glycogen levels of liver, muscle, testis, and tumor tissues of rats

Fikriye POLAT
Egemen DERE
Eylem GÜL
İzzet YELKUVAN
Öztürk ÖZDEMİR
Günsel BİNGÖL
Pages
33–38
Publication Date
2013-01-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Our results showed that the administration of 3-methylcholanthrene, butylated hydroxytoluene, and 3-methylcholanthrene + butylated hydroxytoluene caused statistically significant changes in the glycogen levels of liver, muscle, and testis tissues, and glycogen was stored in tumor tissue.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study examined the effects of separate and combined applications of 3-methylcholanthrene, a polycyclic aromatic hydrocarbon and potent carcinogenic agent, and butylated hydroxytoluene, the antioxidant food additive, on the glycogen levels of liver, muscle, testis, and tumor tissues in rats.
📊
Key Findings & Data:Adult male Wistar albino rats weighing 100-110 g at 8 weeks of age were used in this study.
💡
Conclusion & Impact:Our results showed that the administration of 3-methylcholanthrene, butylated hydroxytoluene, and 3-methylcholanthrene + butylated hydroxytoluene caused statistically significant changes in the glycogen levels of liver, muscle, and testis tissues, and glycogen was stored in tumor tissue.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Key words: Glycogen · 3-methylcholanthrene · butylated hydroxytoluene

Abstract

This study examined the effects of separate and combined applications of 3-methylcholanthrene, a polycyclic aromatic hydrocarbon and potent carcinogenic agent, and butylated hydroxytoluene, the antioxidant food additive, on the glycogen levels of liver, muscle, testis, and tumor tissues in rats. Adult male Wistar albino rats weighing 100-110 g at 8 weeks of age were used in this study. This study consisted of a control group (n = 9) and 3 different experiment groups in which rats were chronically treated with 3-methylcholanthrene (n = 9) or butylated hydroxytoluene (n = 11) or a combination of these agents (n = 14). Rats were intraperitoneally injected with a 200 mg kg-1 dose of butylated hydroxytoluene and a 40 mg kg-1 dose of 3-methylcholanthrene. At the end of the 26-week experimental period, tissues of rats killed via cervical dislocation were placed in 10% trichloroacetic acid for glycogen determination. Our results showed that the administration of 3-methylcholanthrene, butylated hydroxytoluene, and 3-methylcholanthrene + butylated hydroxytoluene caused statistically significant changes in the glycogen levels of liver, muscle, and testis tissues, and glycogen was stored in tumor tissue.

Özet

This study examined the effects of separate and combined applications of 3-methylcholanthrene, a polycyclic aromatic hydrocarbon and potent carcinogenic agent, and butylated hydroxytoluene, the antioxidant food additive, on the glycogen levels of liver, muscle, testis, and tumor tissues in rats. Adult male Wistar albino rats weighing 100-110 g at 8 weeks of age were used in this study. This study consisted of a control group (n = 9) and 3 different experiment groups in which rats were chronically treated with 3-methylcholanthrene (n = 9) or butylated hydroxytoluene (n = 11) or a combination of these agents (n = 14). Rats were intraperitoneally injected with a 200 mg kg-1 dose of butylated hydroxytoluene and a 40 mg kg-1 dose of 3-methylcholanthrene. At the end of the 26-week experimental period, tissues of rats killed via cervical dislocation were placed in 10% trichloroacetic acid for glycogen determination. Our results showed that the administration of 3-methylcholanthrene, butylated hydroxytoluene, and 3-methylcholanthrene + butylated hydroxytoluene caused statistically significant changes in the glycogen levels of liver, muscle, and testis tissues, and glycogen was stored in tumor tissue.

🔖

Cite This Article

POLAT, F., DERE, E., GÜL, E., YELKUVAN, İ., ÖZDEMİR, Ö., BİNGÖL, G. (2013). The effect of 3-methylcholanthrene and butylated hydroxytoluene on glycogen levels of liver, muscle, testis, and tumor tissues of rats. Turkish Journal of Biology, Cilt: 37 - Sayı: 1, 33–38. https://ajindex.com/articles/181779

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (11)

  1. Gressani MK, Rollins LA, Kabler SL et al. Induction of mutations in ki-ras and Ink4a in liver tumors of mice exposed in utero to 3-methylcholanthrene. Carcinogenesis 19: 1045–52, 19 Gressani KM, Kabler SL, O’Sullivan MG et al. Strain-dependent lung tumor formation in mice transplacentally exposed to 3-methylcholanthrene and post-natally exposed to butylated hydroxytoluene. Carcinogenesis 20: 2159–165, 1999.
  2. Demir İ, Demirbağ Z. Polisiklik aromatic hidrokarbonların biyolojik olarak parçalanması. Turk J Biol 23: 293–302, 1999. O’Donnell EP, Zerbe LK, Dwyer-Nield LD et al. Quantitative analysis of early chemically-induced pulmonary lesions in mice of varying susceptibilities to lung tumorigenesis. Cancer Lett 241: 197–202, 2006. Ruddon RW. Cancer Biology. Oxford University Press. New York; 1995.
  3. Chepelev LL, Beshara CS, MacLean PD et al. Polypyrroles as antioxidants: kinetic studies on reactions of bilirubin and biliverdin dimethyl esters and synthetic model compounds with peroxyl radicals in solution. Chemical calculations on selected typical structures. J Org Chem 71: 22–30, 2006.
  4. Aqil F, Ahmad I, Mehmood Z. Antioxidant and free radical scavenging properties of twelve traditionally used Indian medicinal plants. Turk J Biol 30: 177–183, 2006.
  5. Wang X, Witschi H. Mutations of the ki-ras protooncogene in 3-methylcholantrene and urethan-induced and butylated hydroxytoluene-promoted lung tumors of strain A/J and swr mice. Cancer Lett 91: 33–39, 1995. Witschi HP. Promotion of lung tumors in mice. Environ Health Persp 50: 267–273, 1983.
  6. Brown LM, Malkinson AM, Rannels D et al. Compensatory lung growth after partial pneumonectomy enhances lung tumorigenesis induced by 3-methylcholanthrene. Cancer Res 59: 5089–92, 1999. Büyükdoğan M. Kolorektal kanserde genetik ve etyolojik faktörler. Selçuk Tıp Dergisi 25: 171–180, 2009.
  7. Roe JH, Bailey JM, Gray RR et al. Complete removal of glycogen from tissues by extraction with cold trichloroacetic acid solution. J Biol Chem 236, 1961.
  8. Carroll NV, Longley RW, Roe JH. The determination of glycogen in liver and muscle by use of anthrone reagent. J Biol Chem 220: 583–93, 1956.
  9. Kocaçalışkan İ, Akanıl Bingöl N. Biyoistatistik. Nobel Basımevi. Ankara; 2008.
  10. Polat F, Özdemir Ö, Elagöz Ş. Analysis of ki-ras exon 2 gene mutations in 3-methylcholanthrene and butylated hydroxytoluene-induced rat lung tissues. Turk J Biol 32: 277– 82, 2008.
  11. Kabler SL, Wessner LL, McEntee F et al. Ki-ras mutations are an early event and correlate with tumor s