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Journals / Turkish Journal of Biology / 2015 / Cilt: 39 - Sayı: 3

Ultrastructural effects of lead acetate on the spleen of rats

MESURE TÜRKAY
HÜSEYİN TÜRKER
TURAN GÜVEN
Pages
511–516
Publication Date
2015-08-13
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These cytopathologic alterations indicate that lead acetate has some drastic toxicological effects on cellular structures.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this experiment, the ultrastructural effects of orally administered lead acetate on rat (Rattus rattus) spleen were studied.
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Key Findings & Data:Rats weighing approximately 200-250 g were used in this experiment.
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Conclusion & Impact:These cytopathologic alterations indicate that lead acetate has some drastic toxicological effects on cellular structures.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Rats · spleen cells · lead acetate

Abstract

In this experiment, the ultrastructural effects of orally administered lead acetate on rat (Rattus rattus) spleen were studied. Rats weighing approximately 200-250 g were used in this experiment. They were divided into five groups, each one including five rats. Group I served as the normal control group while the others served as the experimental groups. The rats were exposed to oral administrations of lead acetate of 2.5 mg dissolved in 1 mL of drinking water per day. The administration lasted 96, 144, 336, and 432 h. The animals were anesthetized using chloroform inhalation and the peritoneum was stripped open and the spleen was removed and prepared for histological observation using a staining technique. Hypocellular white pulp, enlargement of venous sinusoids, clustering of heterochromatin in nucleus, vacuolation in the cytoplasm, swelling of mitochondria, and the distortion of rough endoplasmic reticulum cisterns were observed upon electron microscopic examination of the spleen tissue cells. These cytopathologic alterations indicate that lead acetate has some drastic toxicological effects on cellular structures.

Özet

In this experiment, the ultrastructural effects of orally administered lead acetate on rat (Rattus rattus) spleen were studied. Rats weighing approximately 200-250 g were used in this experiment. They were divided into five groups, each one including five rats. Group I served as the normal control group while the others served as the experimental groups. The rats were exposed to oral administrations of lead acetate of 2.5 mg dissolved in 1 mL of drinking water per day. The administration lasted 96, 144, 336, and 432 h. The animals were anesthetized using chloroform inhalation and the peritoneum was stripped open and the spleen was removed and prepared for histological observation using a staining technique. Hypocellular white pulp, enlargement of venous sinusoids, clustering of heterochromatin in nucleus, vacuolation in the cytoplasm, swelling of mitochondria, and the distortion of rough endoplasmic reticulum cisterns were observed upon electron microscopic examination of the spleen tissue cells. These cytopathologic alterations indicate that lead acetate has some drastic toxicological effects on cellular structures.

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Cite This Article

TÜRKAY, M., TÜRKER, H., GÜVEN, T. (2015). Ultrastructural effects of lead acetate on the spleen of rats. Turkish Journal of Biology, Cilt: 39 - Sayı: 3, 511–516. https://ajindex.com/articles/181758

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