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Journals / Turkish Journal of Biology / 2015 / Cilt: 39 - Sayı: 5

Machilus thunbergii extract inhibits inflammatory response inlipopolysaccharide-induced RAW264.7 murine macrophages viasuppression of NF-$\kappa$B and p38 MAPK activation

SHU-JU WU
WEN-BIN LEN
Chien-yi HUANG
Chian-jiun LIOU
WEN-CHUNG HUANG
Chwan-fwu LIN
Pages
657–665
Publication Date
2015-12-04
DOI
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⚡ TL;DR(Too Long; Didn't Read)

Taken together, these results suggest that M. thunbergii extract has an anti-inflammatory effect because it reduces levels of proinflammatory cytokines and mediators via the suppression of NF-?B and p38 MAPK activation in RAW 264.7 cells.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Machilus thunbergii Sieb. and Zucc. (Lauraceae) is a medicinal plant used to treat edema and pain in China and Taiwan.
📊
Key Findings & Data:In this study, we evaluated the anti-inflammatory effects of M. thunbergii extract in LPS-stimulated RAW 264.7 cells.
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Conclusion & Impact:Taken together, these results suggest that M. thunbergii extract has an anti-inflammatory effect because it reduces levels of proinflammatory cytokines and mediators via the suppression of NF-?B and p38 MAPK activation in RAW 264.7 cells.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Cyclooxygenase-2 · inducible nitric oxide synthase · Machilus thunbergii

Abstract

Machilus thunbergii Sieb. and Zucc. (Lauraceae) is a medicinal plant used to treat edema and pain in China and Taiwan. The anti-inflammatory effects of M. thunbergii were not clear. In this study, we evaluated the anti-inflammatory effects of M. thunbergii extract in LPS-stimulated RAW 264.7 cells. The cells were pretreated with various doses (12.5 μg/mL-100 μg/mL) of M. thunbergii extract and activated with LPS. We found that 25 μg/mL M. thunbergii extract inhibited nitric oxide (NO), prostaglandin E2 (PGE2), inducible nitric oxide synthetase (iNOS), and cyclooxygenase-2 (COX-2) expression, as well as levels of proinflammatory cytokines including interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α). Furthermore, M. thunbergii extract also suppressed translocation of nuclear transcription factor kappa-B (NF-?B) subunit p65 proteins into the nucleus and induced a dose-dependent inhibition of the phosphorylation of p38 mitogen-activated protein kinase (MAPK). M. thunbergii extract also increased HO-1 and Nrf2 production in a concentration-dependent manner. Taken together, these results suggest that M. thunbergii extract has an anti-inflammatory effect because it reduces levels of proinflammatory cytokines and mediators via the suppression of NF-?B and p38 MAPK activation in RAW 264.7 cells.

Özet

Machilus thunbergii Sieb. and Zucc. (Lauraceae) is a medicinal plant used to treat edema and pain in China and Taiwan. The anti-inflammatory effects of M. thunbergii were not clear. In this study, we evaluated the anti-inflammatory effects of M. thunbergii extract in LPS-stimulated RAW 264.7 cells. The cells were pretreated with various doses (12.5 μg/mL-100 μg/mL) of M. thunbergii extract and activated with LPS. We found that 25 μg/mL M. thunbergii extract inhibited nitric oxide (NO), prostaglandin E2 (PGE2), inducible nitric oxide synthetase (iNOS), and cyclooxygenase-2 (COX-2) expression, as well as levels of proinflammatory cytokines including interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α). Furthermore, M. thunbergii extract also suppressed translocation of nuclear transcription factor kappa-B (NF-?B) subunit p65 proteins into the nucleus and induced a dose-dependent inhibition of the phosphorylation of p38 mitogen-activated protein kinase (MAPK). M. thunbergii extract also increased HO-1 and Nrf2 production in a concentration-dependent manner. Taken together, these results suggest that M. thunbergii extract has an anti-inflammatory effect because it reduces levels of proinflammatory cytokines and mediators via the suppression of NF-?B and p38 MAPK activation in RAW 264.7 cells.

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Cite This Article

WU, S., LEN, W., HUANG, C., LIOU, C., HUANG, W., LIN, C. (2015). Machilus thunbergii extract inhibits inflammatory response inlipopolysaccharide-induced RAW264.7 murine macrophages viasuppression of NF-$\kappa$B and p38 MAPK activation. Turkish Journal of Biology, Cilt: 39 - Sayı: 5, 657–665. https://ajindex.com/articles/181470

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