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Journals / Turkish Journal of Biology / 2020 / Cilt: 44 - Sayı: 1

The effect of R547, a cyclin-dependent kinase inhibitor, on hepatocellular carcinoma cell death

Betül HACIOĞLU
Gökhan KUŞ
Hatice Mehtap KUTLU
Selda KABADERE
Pages
24–33
Publication Date
2020-02-17
DOI
—
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⚡ TL;DR(Too Long; Didn't Read)

After 24 h of culture with 0.1, 1, 10, 50, and 100 ?M doses of R547, the corresponding percentages of live Hep G2 cells were 101%, 94%, 93%, 89%, and 79% (P

📌 3 Key Takeaways

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Objective & Hypothesis:Hepatocellular carcinoma (HCC) is the third main cause of cancer-related death.
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Key Findings & Data:Since inhibition of CDKs gives some guiding ideas for cancer studies, we aimed to determine the possible effects of R547, a cyclin kinase 1-2-4 inhibitor, on proliferation and apoptotic mechanisms of Hep G2 cells (human) and H-4- II-E cells derived from rat HCC.
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Conclusion & Impact:After 24 h of culture with 0.1, 1, 10, 50, and 100 ?M doses of R547, the corresponding percentages of live Hep G2 cells were 101%, 94%, 93%, 89%, and 79% (P
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:R547 · hepatocellular carcinoma · Hep G2

Abstract

Hepatocellular carcinoma (HCC) is the third main cause of cancer-related death. Cyclin-dependent kinases (CDKs) and their cyclin partners regulate the cell cycle. Since inhibition of CDKs gives some guiding ideas for cancer studies, we aimed to determine the possible effects of R547, a cyclin kinase 1-2-4 inhibitor, on proliferation and apoptotic mechanisms of Hep G2 cells (human) and H-4- II-E cells derived from rat HCC. We determined in vitro survival rates with MTT assay, apoptosis with flow cytometry, morphological changes with confocal microscopy, and ultrastructural changes by transmission electron microscopy. Cisplatin was used as a positive control. After 24 h of culture with 0.1, 1, 10, 50, and 100 ?M doses of R547, the corresponding percentages of live Hep G2 cells were 101%, 94%, 93%, 89%, and 79% (P

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Cite This Article

HACIOĞLU, B., KUŞ, G., KUTLU, H. M., KABADERE, S. (2020). The effect of R547, a cyclin-dependent kinase inhibitor, on hepatocellular carcinoma cell death. Turkish Journal of Biology, Cilt: 44 - Sayı: 1, 24–33. https://ajindex.com/articles/181361

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