Journals / Kırıkkale Üniversitesi Tıp Fakültesi Dergisi / 2018 / Cilt: 20 - Sayı: 3

PREVALENCE OF E. coli O157:H7 ISOLATED FROM HUMAN AND ANIMAL SOURCE IN KIRIKKALE PROVINCE

Kırıkkale Yöresinde İnsan ve Hayvan Kaynaklarından İzole Edilen E. coli O157:H7’nin Prevalansı

Pages
256–262
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: Our results indicate that the meat chain from cattle to humans is safe with respect to the E. coli O157:H7 strain.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: Escherichia coli O157:H7 strain is a cause of hemorrhagic colitis and may give rise to severe outbreaks even at a low concentration.
📊
Key Findings & Data:Presence of E. coli O157:H7 was investigated in humans, cattle, animal feed, and ground beef over a one-year year period in order to determine the prevalence in the Kırıkkale region.Material and Methods: All samples were transferred to the microbiology laboratory as rapidly as possible under appropriate and sterile conditions.
💡
Conclusion & Impact:Conclusion: Our results indicate that the meat chain from cattle to humans is safe with respect to the E. coli O157:H7 strain.
🔬Design:Research Article
📍Scope:84 samples
⚙️Variables:Escherichia coli · ELISA · seroprevalans

Abstract

Objective: Escherichia coli O157:H7 strain is a cause of hemorrhagic colitis and may give rise to severe outbreaks even at a low concentration. Transmission may occur through fecal-oral route with contaminated food but direct transmission from personal contact is also possible. Presence of E. coli O157:H7 was investigated in humans, cattle, animal feed, and ground beef over a one-year year period in order to determine the prevalence in the Kırıkkale region.Material and Methods: All samples were transferred to the microbiology laboratory as rapidly as possible under appropriate and sterile conditions. The isolation of E. coli O157:H7 was performed by serotyping with Dynabeads and ELISA methods in stool specimens in 89 patients with gastroenteritis, 108 cattle, 69 different animal broth samples, and 84 samples from ground beef after culture using classical methods. Minced meat samples were kept at -70 degrees until working tests.Results: E. coli O157:H7 was not detected in humans or animals or sources including animal feed and beef carcasses. Conclusion: Our results indicate that the meat chain from cattle to humans is safe with respect to the E. coli O157:H7 strain. On the other hand, other food or water sources may be potential sources for this microorganism.

Özet

Amaç: Escherichia coli O157:H7 suşu hemorajik kolite neden olmakta ve çok düşük konsantrasyonlarda bile ciddi salgınlara yol açabilmektedir. Bulaşma kontamine yiyeceklerin fekal-oral yol ile alınması ile gerçekleşmekte fakat doğrudan insan teması ile de görülebilmektedir. Ülkemizde E. coli O157:H7 prevalansı genel olarak bilinmemekle beraber Kırıkkale bölgesinde de bilinmemektedir. E. coli O157:H7 prevalansını belirlemek amacı ile Kırıkkale bölgesinde insan, besi hayvanı, yem ve et örnekleri bir yıl süre ile araştırıldı.Gereç ve Yöntemler: Bütün örnekler mümkün olan en kısa sürede doğru ve steril şartlarda laboratuvara getirildi. E. coli O157:H7 izolasyonunda 89 gastroenteritli hastanın dışkı örneği, 108 sığır dışkısı, 69 değişik hayvan yemi ve 84 et (kıyma) örneği klasik kültürü takiben Dynabead ve ELISA metotları kullanılarak belirlendi. Kıyma örnekleri çalışma testleri yapılıncaya kadar -70 derecede bekletildi.Bulgular: E. coli O157:H7 araştırılan insan ve hayvanlarda, yem veya et (kıyma) örneklerinde tespit edilemedi. Sonuç: Bulgularımız ahırdan insana uzanan besin zincirinin E. coli O157:H7 suşu açısından güvenli olduğunu göstermiştir. Öte yandan diğer besin ve su kaynakları bu mikroorganizma için potansiyel kaynaklar olabilir.

🔖

Cite This Article

APAN, T. Z., YIDIRIM, M., BAŞALAN, M., KASIMOĞLU, A. (2018). PREVALENCE OF E. coli O157:H7 ISOLATED FROM HUMAN AND ANIMAL SOURCE IN KIRIKKALE PROVINCE. Kırıkkale Üniversitesi Tıp Fakültesi Dergisi, Cilt: 20 - Sayı: 3, 256–262. https://ajindex.com/articles/180646

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (13)

  1. Chattaway MA, Dallman T, Okeke IN, Wain J. Enteroaggregative E. coli O104 from an outbreak of HUS in Germany 2011, could it happen again? J Infect Dev Ctries. 2011;5(6):425-36
  2. Rubino S, Capuccinelli P, Kelvin DJ. Escherichia coli (STEC) serotype O104 outbreak causing haemolytic syndrome (HUS) in Germany and France. J Infect Dev Ctries. 2011;5(6):437-40
  3. Riley LW, Remis RS, Helgerson SD, McGee HB, Wells JG, Davis BR et al. Hemorrhagic colitis associated with a rare Escherichia coli serotype. N Engl J Med. 1983;308(12):681-5
  4. Riley LW. The epidemiological, clinical and microbiological features of hemorrhagic colitis. Ann Rev Microbiol. 1987;41:383-407
  5. Jackson SA, Patel IR, Barnaba T, LeClerc JE, Cebula TA. Investigating the global genomic diversity of Escherichia coli using a multi-genome DNA microarray platform with novel gene prediction strategy. BMC Genomics. 2011;12:349
  6. Ravva SV, Sarreal CZ, Mandrell RE. Identification of protozoa in dairy lagoon wastewater that consume Escherichia coli O157:H7 preferentially. PLoS One. 2010;5:e15671
  7. Griffin PM, Tauxe RV. The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic Escherichia coli and associated hemolytic uremic syndrome. Am J Epidemiol. 1991;13:60-98
  8. Bopp CA, Brenner FW, Wells JG, Strockbine AA. Escherichia, Shigella and Salmonella. In: Murray PR, Baron EJ, Jorgensen JH, Pfaller MA, Yolken RH, eds. Manual of Clinical Microbiology. 7th ed. Washington D.C. AMS Press, 2003:654-71
  9. Karmali MA, Petric M, Lim C, Flemming PC, Arbus GS, Lior H. The association between idiopathic hemolytic uremic syndrome and infection by verotoxin producing Escherichia coli. J Infect Dis. 1985;151(5):775-82
  10. Hascelik G, Akan OA, Diker S, Baykal M. Campylobacter and Enterohemorrhagic Escherichia coli associated gastroenteritis in Turkish children. J Diarrheal Dis Res Dec. 1991;9(4):315-7
  11. Amisano G, Fornasero S, Migliaretti G, Caramello S, Tarasco V, Savino F. Diarrheagenic Escherichia coli in acute gastroenteritis in infants in north-west Italy. New Microbiologica. 2011;34(1):45-51
  12. Käppeli U, Hächler H, Giezendanner N, Beutin L, Stephan R. Human infection with non-O157 shiga toxin-producing Escherichia coli, Switzerland, 2000-2009. Emerg Infect Dis. 2011;17(2):180-5
  13. Chigor VN, Umoh VJ, Smith SI, Igbinosa EO, Okoh AI. Multidrug resistance and plasmid patterns of Escherichia coli O157 and other E. coli isolated from diarrhoeal stools and surface wate