SavedAdd your journal

Journals / Turkish Journal of Agriculture and Forestry / 2009 / Cilt: 33 - Sayı: 1

Genetic Diversity of Wilt and Root Rot Pathogens of Chickpea, as Assessed by RAPD and ISSR

Harun BAYRAKTAR
F. Sara DOLAR
Pages
1–10
Publication Date
2009-01-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results show that both analyses were suitable for the evaluation of genetic polymorphisms among fungal populations.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Intra- and interspecific polymorphisms among fungal pathogens that cause wilt and root rot on chickpea were investigated by using 30 RAPD (random amplified polymorphic DNA) and 20 ISSR (inter-simple sequence repeats) primers.
📊
Key Findings & Data:Additionally, high cophenetic correlation values for RAPD and ISSR data, using the MXCOPH algorithm, were observed (0.983 and 0.987, respectively).
💡
Conclusion & Impact:The results show that both analyses were suitable for the evaluation of genetic polymorphisms among fungal populations.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Chickpea · root rot · genetic diversity

Abstract

Intra- and interspecific polymorphisms among fungal pathogens that cause wilt and root rot on chickpea were investigated by using 30 RAPD (random amplified polymorphic DNA) and 20 ISSR (inter-simple sequence repeats) primers. UPGMA (unweighted pair group method with arithmetic average) cluster analysis of RAPD and ISSR datasets using Dice's coefficient differentiated all fungal isolates from each other and revealed considerable genetic variability between the isolates. The isolates that were not easily separable using morphological characteristics were classified again according to their branching in dendrograms. A partial correlation was observed between cluster analysis and geographic origin of Fusarium solani isolates. Additionally, high cophenetic correlation values for RAPD and ISSR data, using the MXCOPH algorithm, were observed (0.983 and 0.987, respectively). The results show that both analyses were suitable for the evaluation of genetic polymorphisms among fungal populations.

Özet

Intra- and interspecific polymorphisms among fungal pathogens that cause wilt and root rot on chickpea were investigated by using 30 RAPD (random amplified polymorphic DNA) and 20 ISSR (inter-simple sequence repeats) primers. UPGMA (unweighted pair group method with arithmetic average) cluster analysis of RAPD and ISSR datasets using Dice's coefficient differentiated all fungal isolates from each other and revealed considerable genetic variability between the isolates. The isolates that were not easily separable using morphological characteristics were classified again according to their branching in dendrograms. A partial correlation was observed between cluster analysis and geographic origin of Fusarium solani isolates. Additionally, high cophenetic correlation values for RAPD and ISSR data, using the MXCOPH algorithm, were observed (0.983 and 0.987, respectively). The results show that both analyses were suitable for the evaluation of genetic polymorphisms among fungal populations.

🔖

Cite This Article

BAYRAKTAR, H., DOLAR, F. S. (2009). Genetic Diversity of Wilt and Root Rot Pathogens of Chickpea, as Assessed by RAPD and ISSR. Turkish Journal of Agriculture and Forestry, Cilt: 33 - Sayı: 1, 1–10. https://ajindex.com/articles/180190

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (7)

  1. Abd-Elsalam, K.A., F. Schnieder, M.S. Khalil, A. Asran-Amal and J.A. Verreet. 2003. Use of AFLP fingerprinting to analyze genetic variation within and between populations of Fusarium spp. Derived from Egyptian cotton cultivars. J. Plant Pathol. 85: 99- 103. Abou-Zeid, N.M. and H. Hallila. 2003. Current status of chickpea diseases in Egypt. In: International Chickpea Conference, Chickpea Research for Millennium Raipur, Chhattisgarh, January, pp. 156- 166, India. Balmas, V., B. Scherm, P.D. Primo, D. Rau, A. Marcello and Q. Migheli. 2005. Molecular characterisation of vegetative compatibility groups in Fusarium oxysporum f. sp. radicis-lycopersici and f. sp. lycopersici by random amplification of polymorphic DNA and microsatellite-primed PCR. Eur. J. Plant Pathol. 111: 1-8.
  2. Barve, M.P., M.P. Haware, M.N. Sainani, P.K. Ranjekar and V.S. Gupta. 2001. Potential of microsatellites to distinguish four races of Fusarium oxysporum f. sp. ciceri prevalent in India. Theor. Appl. Genet. 102: 138-147.
  3. Bayraktar, H., F.S. Dolar and S. Maden. 2008. Use of RAPD and ISSR markers in detection of genetic variation and population structure among Fusarium oxysporum f. sp. ciceris isolates on chickpea in Turkey. J. of Phytopathol. 156: 146-154.
  4. Chakrabarti, A., P.K. Mukherjee, P.D. Sherkhane, A.S. Bhagwat and N.B.K. Murthy. 2001. A simple and rapid molecular method for distinguishing between races of Fusarium oxysporum f. sp. ciceris from India. Curr. Sci. 80: 571-575. 10 Khalil M.S., M.A. Abdel-Sattar, I.N. Aly, K.A. Abd-Elsalam and J.A. Verreet. 2003. Genetic affinities of Fusarium spp. and their correlation with origin and pathogenicity. Afr. J. Biotech. 2: 109- 113. Lu, G., P.F. Cannon, A. Reid and C.M. Simmons. 2004. Diversity and molecular relationships of endophytic Colletotrichum isolates from the Iwokrama Forest Reserve, Guyana. Mycol. Res. 108: 53-63.
  5. Meyer, W., T.G. Mitchell, E.Z. Freedman and R. Vilgays. 1993. Hybridization probes for conventional DNA fingerprinting used as single primers in the polymerase chain reaction to distinguish strains of Cryptococcus neoformans. J. Clin. Microbiol. 31: 2274- 2280. Mishra, K.P., R.T.V. Fox and A. Culham. 2003. Inter-simple sequence repeat and aggressiveness analyses revealed high genetic diversity, recombination and long-range dispersal in Fusarium culmorum. Ann. Appl. Biol. 143: 291-301.
  6. Nene, Y.L. and M.P. Haware. 1980. Screening chickpea for resistance to wilt. Plant Dis. 66: 379-380.
  7. Nene, Y.L., V.K. Sheila and S.B. Shar