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Journals / Turkish Journal of Agriculture and Forestry / 2015 / Cilt: 39 - Sayı: 1

Production of dihaploids in durum wheat using Imperata cylindrica L. mediated chromosome elimination

Nafiz ÇELİKTAŞ
Murat TİRYAKİOĞLU
Ersin CAN
Duygu KUTLAY
Rüştü HATİPOĞLU
Pages
48–54
Publication Date
2015-03-15
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Cytological observations revealed that all the regenerants had 2n = 2x = 14 chromosomes.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The possibility of obtaining dihaploid durum wheat (Triticum durum L.) plants by interspecific hybridization with Imperata cylindrica L. was studied.
📊
Key Findings & Data:Ten local varieties and 1 commercial durum wheat variety (Triticum durum L., 2n = 4x = 28; AABB) were used as maternal lines.
💡
Conclusion & Impact:Cytological observations revealed that all the regenerants had 2n = 2x = 14 chromosomes.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Imperata cylindrica L. · interspecific cross · Triticum durum L.

Abstract

The possibility of obtaining dihaploid durum wheat (Triticum durum L.) plants by interspecific hybridization with Imperata cylindrica L. was studied. Ten local varieties and 1 commercial durum wheat variety (Triticum durum L., 2n = 4x = 28; AABB) were used as maternal lines. Imperata cylindrica L. (2n = 2x = 20) ecotypes sampled from different districts of Hatay were used as the pollen source. Isolated embryos were placed in an MS medium for plant regeneration 14 days after the interspecific hybridization. The highest average seed setting, embryo formation, and dihaploid plant regeneration for the different hybrid combinations were determined as 31.1%, 15.5%, and 11.5%, respectively. Cytological observations revealed that all the regenerants had 2n = 2x = 14 chromosomes.

Özet

The possibility of obtaining dihaploid durum wheat (Triticum durum L.) plants by interspecific hybridization with Imperata cylindrica L. was studied. Ten local varieties and 1 commercial durum wheat variety (Triticum durum L., 2n = 4x = 28; AABB) were used as maternal lines. Imperata cylindrica L. (2n = 2x = 20) ecotypes sampled from different districts of Hatay were used as the pollen source. Isolated embryos were placed in an MS medium for plant regeneration 14 days after the interspecific hybridization. The highest average seed setting, embryo formation, and dihaploid plant regeneration for the different hybrid combinations were determined as 31.1%, 15.5%, and 11.5%, respectively. Cytological observations revealed that all the regenerants had 2n = 2x = 14 chromosomes.

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Cite This Article

ÇELİKTAŞ, N., TİRYAKİOĞLU, M., CAN, E., KUTLAY, D., HATİPOĞLU, R. (2015). Production of dihaploids in durum wheat using Imperata cylindrica L. mediated chromosome elimination. Turkish Journal of Agriculture and Forestry, Cilt: 39 - Sayı: 1, 48–54. https://ajindex.com/articles/180016

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