SavedAdd your journal

Journals / Turkish Journal of Agriculture and Forestry / 2012 / Cilt: 36 - Sayı: 3

Genetic characterization of Pyrenophora graminea isolates and the reactions of some barley cultivars to leaf stripe disease under greenhouse conditions

Harun BAYRAKTAR
Kadir AKAN
Pages
329–339
Publication Date
2012-03-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These results indicated that the Turkish isolates of P. graminea were genetically homogeneous and may be derived from a single gene pool.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The genetic diversity of Pyrenophora graminea, the causal agent of barley leaf stripe disease, was characterized by restriction fragment length polymorphism (RFLP) of the rDNA internal transcribed spacer region and intersimple sequence repeat (ISSR) analysis.
📊
Key Findings & Data:A total of 45 P. graminea isolates recovered from different geographic origins and barley cultivars or genotypes produced identical RFLP banding patterns with HinfI, MspI, Hin6I and BsuRI restriction enzymes.
💡
Conclusion & Impact:These results indicated that the Turkish isolates of P. graminea were genetically homogeneous and may be derived from a single gene pool.
🔬Design:Lab & Field Experiment
📍Scope:2001 Verileri
⚙️Variables:Key words: Barley (Hordeum spp.) · barley leaf stripe · genetic resistance

Abstract

The genetic diversity of Pyrenophora graminea, the causal agent of barley leaf stripe disease, was characterized by restriction fragment length polymorphism (RFLP) of the rDNA internal transcribed spacer region and intersimple sequence repeat (ISSR) analysis. A total of 45 P. graminea isolates recovered from different geographic origins and barley cultivars or genotypes produced identical RFLP banding patterns with HinfI, MspI, Hin6I and BsuRI restriction enzymes. ISSR analysis indicated that there was little genetic variability among the isolates. A dendrogram based on Jaccard's coefficient separated the isolates into 4 clusters with 12% dissimilarity. In the dendrogram, the isolates collected from different geographic regions in Turkey were clustered separately from the Italian isolates. These results indicated that the Turkish isolates of P. graminea were genetically homogeneous and may be derived from a single gene pool. In addition, 13 isolates were tested for pathogenic variability on 48 barley cultivars. The cultivars Durusu, Balkan 96 (Igri), Çumra 2001 and Anadolu 98 were found to be resistant to all leaf stripe isolates.

Özet

The genetic diversity of Pyrenophora graminea, the causal agent of barley leaf stripe disease, was characterized by restriction fragment length polymorphism (RFLP) of the rDNA internal transcribed spacer region and intersimple sequence repeat (ISSR) analysis. A total of 45 P. graminea isolates recovered from different geographic origins and barley cultivars or genotypes produced identical RFLP banding patterns with HinfI, MspI, Hin6I and BsuRI restriction enzymes. ISSR analysis indicated that there was little genetic variability among the isolates. A dendrogram based on Jaccard's coefficient separated the isolates into 4 clusters with 12% dissimilarity. In the dendrogram, the isolates collected from different geographic regions in Turkey were clustered separately from the Italian isolates. These results indicated that the Turkish isolates of P. graminea were genetically homogeneous and may be derived from a single gene pool. In addition, 13 isolates were tested for pathogenic variability on 48 barley cultivars. The cultivars Durusu, Balkan 96 (Igri), Çumra 2001 and Anadolu 98 were found to be resistant to all leaf stripe isolates.

🔖

Cite This Article

BAYRAKTAR, H., AKAN, K. (2012). Genetic characterization of Pyrenophora graminea isolates and the reactions of some barley cultivars to leaf stripe disease under greenhouse conditions. Turkish Journal of Agriculture and Forestry, Cilt: 36 - Sayı: 3, 329–339. https://ajindex.com/articles/179983

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (13)

  1. Aktaş H (1984) Spread of leaf spots in barley growing areas in Turkey. In: Proc 6th Congr Mediterr Phytopathol Union, Cairo, Egypt, pp. 338-441.
  2. Almeida AMR, Abdelnoor RV, Arias CAA, Carvalho VP, Jacoud Filho DS, Marin SRR, Benato LC, Pinto MC, Carvalho CGP (2003) Genotypic diversity among Brazilian isolates of Macrophomina phaseolina revealed by RAPD. Fitopatol Bras 28: 279-285.
  3. Arabi MIE, Jawhar M (2007) Heterogeneity in Pyrenophora graminea as revealed by ITS-RFLP. J Plant Pathol 89: 391-395.
  4. Arabi MIE, Jawhar M, Al-Safadi B, MirAli N (2004) Yield response of barley to leaf stripe (Pyrenophora graminea) under experimental conditions in southern Syria. J Phytopathol 152: 519-523.
  5. Arabi MIE, MirAli N, Jawhar M, Al-Safadi B (2002) Diff erentiation of Drechslera graminea isolates by cultural characters and SDS- PAGE. J Plant Pathol 84: 153-156.
  6. Bakonyi J, Justesen AF (2007) Genetic relationship of Pyrenophora graminea, P. teres f. maculata and P. teres f. teres assessed by RAPD analysis. J Phytopathol 155: 76-83.
  7. Balmas V, Scherm B, Ghignone S, Mohamed-Salem AO, Cacciola SO, Migheli Q (2005) Characterization of Phoma tracheiphila by RAPD-PCR, microsatellite-primed PCR and ITS rDNA sequencing and development of specifi c primers for in planta PCR detection. Eur J Plant Pathol 111: 235-247.
  8. Bayraktar H, Dolar FS (2009) Genetic diversity of wilt and root rot pathogens of chickpea, as assessed by RAPD and ISSR. Turk J Agric For 33: 1-10.
  9. Bayraktar H, Dolar FS, Tör M (2007) Determination of genetic diversity within Ascochyta rabiei (Pass.) Labr., the pathogen of ascochyta blight of chickpea in Turkey. J Plant Pathol 89: 341-347.
  10. Bembelkacem A, Boulif M, Amri A, Ceccarelli S (2000) Variation in the pathogenicity of 20 Algerian isolates of Pyrenophora graminea Ito & Kur. on nine barley (Hordeum vulgare L.) varieties. Phytopathol Mediterr 39: 389-395.
  11. Chadha S, Gopalakrishna T (2007) Comparative assessment of REMAP and ISSR marker assays for genetic polymorphism studies in Magnaporthe grisea. Curr Sci 93: 688-692.
  12. Cooke DEL, Drenth A, Duncan JM, Wagels G, Brasier CM (2000) A molecular phylogeny of Phytophthora and related oomycetes. Fungal Genet Biol 30: 17-32.
  13. Çetin L, Albustan S, Düsünceli F, Tosun H, Akar T (1995) Determination of resistance of barley breeding materials developed for Central Anatolia to barley leaf stripe (Pyrenophora graminea). In: VII. Türkiye Fitopatoloji Kongresi, Adana, p.126. Damgacı E, Aktuna İ (1983) Th e investigations on the establish