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Journals / Turkish Journal of Agriculture and Forestry / 2015 / Cilt: 39 - Sayı: 4

Investigation of root-knot nematode (Meloidogyne spp.) resistance in almond rootstocks with DNA markers

Sultan Bay TÜRKOĞLU
Nergiz ÇOBAN
Ali ÖZKAN
Halit Seyfettin ATLI
Canan CAN
Pages
563–571
Publication Date
2015-08-13
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was found that plgms19 exhibited 76%, 77.01%, 73.13%, and 86.6% inheritance of resistance in the Ferragnes × Pissardi nigra, Ferragnes × Myrobalan, Ferragnes × GN22, and Nemaguard × GF677 F1 populations, respectively.

📌 3 Key Takeaways

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Objective & Hypothesis:Seven almond varieties, Ferragnes, Nemaguard, AB3 (Amygdalus orientalis Mill.), GN22, GF677, Myrobalan(Prunus cerasifera),and Pissardi nigra, and F1 crossbreed almonds, Ferragnes × Pissardi nigra, Ferragnes × Myrobalan, Ferragnes × GN22, Nemaguard × GF677, Myrobalan × AB3 (Prunus dulcis Mill.), Myrobalan × Ferragnes, Pissardi nigra × AB3, and GF677 × Myrobalan, were used to determine resistance against root-knot nematode (RKN) (Meloidogyne spp.) using DNA markers.
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Key Findings & Data:Parental lines and 316 F1 plants were tested with SSR (plgms8, plgms9, and plgms19) and STS (STS-OPS14a, STS-834b) markers.
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Conclusion & Impact:It was found that plgms19 exhibited 76%, 77.01%, 73.13%, and 86.6% inheritance of resistance in the Ferragnes × Pissardi nigra, Ferragnes × Myrobalan, Ferragnes × GN22, and Nemaguard × GF677 F1 populations, respectively.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Almond · breeding · DNA marker

Abstract

Seven almond varieties, Ferragnes, Nemaguard, AB3 (Amygdalus orientalis Mill.), GN22, GF677, Myrobalan(Prunus cerasifera),and Pissardi nigra, and F1 crossbreed almonds, Ferragnes × Pissardi nigra, Ferragnes × Myrobalan, Ferragnes × GN22, Nemaguard × GF677, Myrobalan × AB3 (Prunus dulcis Mill.), Myrobalan × Ferragnes, Pissardi nigra × AB3, and GF677 × Myrobalan, were used to determine resistance against root-knot nematode (RKN) (Meloidogyne spp.) using DNA markers. Parental lines and 316 F1 plants were tested with SSR (plgms8, plgms9, and plgms19) and STS (STS-OPS14a, STS-834b) markers. It was found that plgms19 exhibited 76%, 77.01%, 73.13%, and 86.6% inheritance of resistance in the Ferragnes × Pissardi nigra, Ferragnes × Myrobalan, Ferragnes × GN22, and Nemaguard × GF677 F1 populations, respectively. No resistance was detected in the Nemaguard × GF677 population with the plgms8 marker. Since the plgms19 and STS-834b primers were found to be effective at high ratios in determining resistance to RKN in the Nemaguard × GF677 F1 population, the use of these markers could be included in breeding studies. It was also detected that the STS-OPS14a primer is appropriate to use for determining the sensitivity to RKN in almond rootstocks.

Özet

Seven almond varieties, Ferragnes, Nemaguard, AB3 (Amygdalus orientalis Mill.), GN22, GF677, Myrobalan(Prunus cerasifera),and Pissardi nigra, and F1 crossbreed almonds, Ferragnes × Pissardi nigra, Ferragnes × Myrobalan, Ferragnes × GN22, Nemaguard × GF677, Myrobalan × AB3 (Prunus dulcis Mill.), Myrobalan × Ferragnes, Pissardi nigra × AB3, and GF677 × Myrobalan, were used to determine resistance against root-knot nematode (RKN) (Meloidogyne spp.) using DNA markers. Parental lines and 316 F1 plants were tested with SSR (plgms8, plgms9, and plgms19) and STS (STS-OPS14a, STS-834b) markers. It was found that plgms19 exhibited 76%, 77.01%, 73.13%, and 86.6% inheritance of resistance in the Ferragnes × Pissardi nigra, Ferragnes × Myrobalan, Ferragnes × GN22, and Nemaguard × GF677 F1 populations, respectively. No resistance was detected in the Nemaguard × GF677 population with the plgms8 marker. Since the plgms19 and STS-834b primers were found to be effective at high ratios in determining resistance to RKN in the Nemaguard × GF677 F1 population, the use of these markers could be included in breeding studies. It was also detected that the STS-OPS14a primer is appropriate to use for determining the sensitivity to RKN in almond rootstocks.

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Cite This Article

TÜRKOĞLU, S. B., ÇOBAN, N., ÖZKAN, A., ATLI, H. S., CAN, C. (2015). Investigation of root-knot nematode (Meloidogyne spp.) resistance in almond rootstocks with DNA markers. Turkish Journal of Agriculture and Forestry, Cilt: 39 - Sayı: 4, 563–571. https://ajindex.com/articles/179624

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References (12)

  1. Akalın Ş (1952). Büyük Bitkiler Kılavuzu. Cilt 1. Ankara, Turkey: Güzel Sanatlar Matbaası (in Turkish). Atlı HS (2010). GF677 ve GN22 Badem Anaçlarının Çelikle Çoğaltılma Durumlarının Karşılaştırılması. Gaziantep, Turkey: Antepfıstığı Araştırma Enstitüsü Müdürlüğü (in Turkish). Bayrak S, Yılmaz Ö (2009).
  2. Ceviz-badem yetiştiriciliği. Ankara, Turkey: Rekmay Reklam ve Tanıtım Ltd. (in Turkish). Boerma HR, Hussey RS (1992). Breeding plants for resistance to nematodes. J Nematol 24: 242–252.
  3. Claverie M, Bosselut N, Lecouls AC, Voisin R, Poizat C, Dirlewanger E, Kleinhentz M, Lafargue B, Laigret F, Esmenjaud D (2004a). Location of independent root-knot nematode resistance genes in plum and peach. Theor Appl Genet 108: 765–773.
  4. Claverie M, Dirlewanger E, Bosselut N, Ghelder VC, Voisin R, Kleinhentz M, Lafargue B, Abad P, Rosso MN, Chalhoub B et al. (2011). The Ma gene for complete-spectrum resistance to Meloidogyne species in Prunus is a TNL with a huge repeated C-terminal post-LRR region. Plant Physiol 156: 779–792.
  5. Claverie M, Dirlewanger E, Cosson P, Bosselut N, Lecouls AC, Voisin R, Kleinhentz M, Lafargue B, Caboche C, Chalhoub B et al. (2004b). High-resolution mapping and chromosome landing at the root-knot nematode resistance locus Ma from Myrobalan plum using a large-insert BAC DNA library. Theor Appl Genet 109: 1318–1327.
  6. Collard BCY, Jahufer MZZ, Brouwer JB, Pang ECR (2005). An introduction to markers, quantitative trait loci (QTL) mapping, and marker-assisted selection for crop improvement: the basic concepts. Euphytica 142: 169–196.
  7. Devran Z, Söğüt MA (2009). Distribution and identification of root- knot nematodes from Turkey. J Nematol 41: 128–133.
  8. Devran Z, Söğüt MA (2011). Characterizing races of Meloidogyne incognita, M. javanica and M. arenaria in the West Mediterranean region of Turkey. Crop Prot 30: 451–455.
  9. Doyle JJ, Doyle JL (1987). A rapid isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19: 11–15.
  10. Esmenjaud D, Minot JC, Voisin R (1996). Effect of durable inoculum pressure and high temperature on root galling, nematode numbers and survival of Myrobalan plum genotypes (Prunus cerasifera Ehr.) highly resistant to Meloidogyne spp. Theor Appl Genet 92: 873–879.
  11. Esmenjaud D, Minot JC, Voisin R, Pinochet J, Simard MH, Salesses G (1997). Differential response to root-knot nematodes in Prunus species and correlative genetic implications. J Nematol 29: 372–380.
  12. Esmenjaud D, Minot JC, Voisin R, Salesses G, Poupet R, Onesto