SavedAdd your journal

Journals / Tarım Bilimleri Dergisi / 2014 / Cilt: 20 - Sayı: 3

-

Dayanıklılık Geni Cre1’in Akdeniz Tahıl kist nematodu, Heterodera latipons’a Franklin Karşı Etkinliğinin Araştırılması

Mustafa İMREN
Ece Börteçine KASAPOĞLU
Abdelfattah DABABAT
Halil TOKTAY
İbrahim Halil ELEKCİOĞLU
Pages
261–268
Publication Date
2014-08-14
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Within this study, the resistant gene, Cre1, was not found to be completely resistant against H. latipons, but its 78% efficiency rate was found to be successful: therefore, it was concluded that it can be used against H. latipons in national wheat breeding programs.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Cereal cyst nematodes (Heterodera avenae group) are important pests of wheat; they include the three most important species of cereal cyst nematodes: Heterodera avenae Wollenweber, H. latipons Franklin and H. filipjevi (Madzhidov) in Turkey that were determined in different wheat growing areas.
📊
Key Findings & Data:In this study, the efficiency of the resistance gene, Cre1, against the population of H. latipons from Gaziantep (Karkamıs) was investigated.
💡
Conclusion & Impact:Within this study, the resistant gene, Cre1, was not found to be completely resistant against H. latipons, but its 78% efficiency rate was found to be successful: therefore, it was concluded that it can be used against H. latipons in national wheat breeding programs.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Tahıl kist nematodu · Heterodera latipons · Buğday

Abstract

Cereal cyst nematodes (Heterodera avenae group) are important pests of wheat; they include the three most important species of cereal cyst nematodes: Heterodera avenae Wollenweber, H. latipons Franklin and H. filipjevi (Madzhidov) in Turkey that were determined in different wheat growing areas. It was reported that Mediterranean cereal cyst nematode, Heterodera latipons which is widely found in the centre of the durum wheat growing area -South-eastern Anatolia regionin our country was reaching the economical threshold in many locations. It is known that the use of resistant/tolerant wheat varieties is the most effective controlling method against cyst nematodes in the wheat cultivation. Nowadays, nine different resistance genes (Cre genes), which are transferred from wild wheat species to wheat cultivated forms are available. In this study, the efficiency of the resistance gene, Cre1, against the population of H. latipons from Gaziantep (Karkamıs) was investigated. In the study, 38 genotypes carrying Cre1 gene and 26 genomes lacking resistance genes; this gives a total 64 wheat genotypes which are produced by parental crossing of SILVERSTAR containing the resistance gene, Cre1, in its genome, SOKOLL, CALINGIRI, GOLDMARKER, CROC_1/AE.SQUARROSA(224)//OPATA, FRAME, STYLET and PASTOR were used against H. latipons in the experiment. Among the 64 genotypes; 20 were resistant, 32 were moderately resistant and 12 were moderately susceptible to the nematode. Ten resistant, 20 moderately resistant and 8 moderately susceptible lines were determined against H. latipons that these 38 lines carried the Cre1 gene in their genome. Within this study, the resistant gene, Cre1, was not found to be completely resistant against H. latipons, but its 78% efficiency rate was found to be successful: therefore, it was concluded that it can be used against H. latipons in national wheat breeding programs. Furthermore, PASTOR, SOKOLL and CROC_1/AE.SQUARROSA(224)//OPATA wheat genotypes were determined as good resistance source against H. latipons.

Özet

Tahıl kist nematodları (Heterodera avenae group) buğdayın önemli zararlılarından biri olup, Türkiye’de Heterodera avenae Wollenweber, H. latipons Franklin ve H. filipjevi (Madzhidov) Stone olmak üzere üç önemli türü farklı buğday üretim alanlarında saptanmıştır. Ülkemizin makarnalık buğdayın merkezi olan Güneydoğu Anadolu Bölgesi’nde Akdeniz tahıl kist nematodu, Heterodera latipons’un yaygın olarak bulunduğu, bölge genelinde birçok lokasyonda ekonomik zarar eşiğinin üstünde popülasyona sahip olduğu rapor edilmiştir. Buğday yetiştiriciliğinde kist nematodları ile en etkin mücadele yöntemi dayanıklı/tolerans çeşit kullanımı olarak bilinmektedir. Günümüzde buğdayın yabani türlerinden kültür formlarına aktarılmış dokuz farklı dayanıklılık geni (Cre genleri) mevcuttur. Bu çalışmada dayanıklılık geni Cre1’in H. latipons’un Gaziantep (Karkamış) popülasyonuna karşı etkinliği araştırılmıştır. Çalışmada genomunda dayanıklılık geni Cre1 bulunan SILVERSTAR buğday çeşidinin yanı sıra SOKOLL, CALINGIRI, GOLDMARKER, FRAME, STYLET, PASTOR ve CROC_1/AE.SQUARROSA(224)//OPATA çeşitlerine ait ebeveynlerin melezlenmesi ile geliştirilen 38 adeti dayanıklılık geni taşıyan, 26 adeti dayanıklılık geni taşımayan toplam 64 adet buğday genotipi H. latipons’a karşı denemeye alınmıştır. Çalışma sonucunda 64 adet genotipten 20’si nematoda dayanıklı, 32’si orta dayanıklı ve 12’sinin ise orta hassas olduğu tespit edilmiştir. Genomunda Cre1 geni taşıyan 38 melezden ise 10’nun H. latipons’a karşı dayanıklı, 20’sinin orta dayanıklı ve 8’nin ise orta hassas olduğu saptanmıştır. Bu çalışma ile dayanıklılık geni Cre1’in H. latipons’un Gaziantep (Karkamış) popülasyonuna karşı mutlak bir dayanıklılığa sahip olmamakla birlikte %78 etkinlik oranı ile başarılı olduğu ve ulusal buğday ıslah programlarında H. latipons’a karşı kullanılabileceği sonucuna varılmıştır. Ayrıca, PASTOR, SOKOLL ve CROC_1/AE.SQUARROSA(224)//OPATA buğday genotiplerinin H. latipons’a karşı iyi bir dayanıklılık kaynağına sahip olduğu saptanmıştır

🔖

Cite This Article

İMREN, M., KASAPOĞLU, E. B., DABABAT, A., TOKTAY, H., ELEKCİOĞLU, İ. H. (2014). -. Tarım Bilimleri Dergisi, Cilt: 20 - Sayı: 3, 261–268. https://ajindex.com/articles/178533

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (7)

  1. Agrios G N (1997). Plant Pathology, fourth edition. Academic Press Inc., London Akar T, Calıskan M, Nicol J M, Uranbey S, Sahin E, Yazar S, William M & Braun H J (2009). Molecular characterization of Cereal Cyst Nematode diagnostic markers Cre1 and Cre3 in some winter wheat germplasm and their potential use against Heterodera filipjevi, Field Crops Research 114(2): 320-323
  2. Barloy D, Lemoine J, Abelard P, Tanguy A M, Rivoal R & Jahier J (2007). Marker-assisted Pyramiding of Two Cereal Cyst Nematode Resistance Genes From Aegilops variabilis in Wheat. Molecular Breeding 20: 31–40
  3. Bekal S, Jahier J & Rivoal R (1998). Host response of different Triticeae to species of the cereal cyst nematode complex in relation to breeding resistant durum wheat. Fundamental Applied Nematology 21: 359-370 Boerma H R & Hussey R S (1992). Breeding Plants for Resistance to Nematodes. Journal of Nematology 24(2): 242–252 Cook R & Rivoal R (1998). Genetics of resistance and parasitism. In: Sharma, S. B. (Ed.). The Cyst Nematodes. Chapman and Hall, London Cook R & Noel G R (2002). Cyst nematodes: Globodera and Heterodera Species, In: Plant Resistance to Parasitic Nematodes (Eds. Star J L, Cook R & Bridge J.), CAB International, pp. 71 – 105 İmren M, Toktay H, Özarslandan A, Nicol J M & Elekcioğlu I H (2012). Güneydoğu Anadolu Bölgesi tahıl alanlarında Tahıl kist nematodu, Heterodera avenae group türlerinin belirlenmesi. Türkiye Entomoloji Dergisi 36(2): 265-275
  4. İmren M, Toktay H, Bozbuğa R, Erginbaş Orakçı G, Dababat A & Elekcioğlu İ H (2013). Identification of genetic resistance to cereal cyst nematodes; Heterodera avenae (Wollenweber, 1924), H. filipjevi (Madzhidov, 1981) Stelter and H. latipons (Franklin, 1969) in some international bread wheat germplasms. Turkish Journal of Entomology 37(3): 277-282
  5. Kort J (1972). Nematode diseases of cereals of temperate climates, In: Webster, J. M. (Ed.). Economic Nematology. Academic Press, New York, 97–126
  6. Majnik J, Ogbonnaya F C, Moullet O & Lagudah E S (2003). The Cre1 and Cre3 Nematode Resistance Genes are Located at Homeologous Loci in the Wheat Genome. The American Phytapathological Society. MPMI 16(12): 1135-1144
  7. Nicol J M, Rivoal R, Bolat N, Aktas H, Braun H J, Mergoum M, Yıldırım A F, Bağcı A, Elekcioğlu I H & Yahyaoui A (2002a) The frequency and diversity of the cyst and lesion nematodes on wheat in the Turkish Central Anatolian Plateau. Nematology 4(2): 272 Nicol, J M (2002b). Important nematode pests. In: Curtis, B. C., Rajaram, S.