SavedAdd your journal

Journals / Turkish Journal of Zoology / 2015 / Cilt: 39 - Sayı: 3

Effects of the pyrethroid insecticide deltamethrin on the hemocytes of Galleria mellonella

DENİZ KURT
Deniz KURT
TAMER KAYIŞ
Pages
452–457
Publication Date
2015-08-13
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

We conclude that deltamethrin affected THCs and caused the induction of micronucleus formation via genotoxic damage in G. mellonella.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In the present study, the effects of deltamethrin on cell-mediated immune response and genotoxic damage of Galleria mellonella (Lepidoptera: Pyralidae) were investigated.
📊
Key Findings & Data:Different concentrations of deltamethrin (5, 20, 50, 100, and 150 µg) were administered to G. mellonella at 24, 48, 72, and 96 h.
💡
Conclusion & Impact:We conclude that deltamethrin affected THCs and caused the induction of micronucleus formation via genotoxic damage in G. mellonella.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Deltamethrin · Galleria mellonella · hemocyte

Abstract

In the present study, the effects of deltamethrin on cell-mediated immune response and genotoxic damage of Galleria mellonella (Lepidoptera: Pyralidae) were investigated. Different concentrations of deltamethrin (5, 20, 50, 100, and 150 µg) were administered to G. mellonella at 24, 48, 72, and 96 h. Five types of hemocytes were identified in G. mellonella: prohemocytes, plasmatocytes, granulocytes, spherulocytes, and oenocytes. Total hemocyte counts (THCs) were significantly affected by deltamethrin. At 72 h, THCs of the treated group had significantly decreased compared with the control groups at high deltamethrin concentrations (50, 100, and 150 µg); however, at 96 h, THCs had significantly increased at deltamethrin concentrations of 100 and 150 µg. Micronucleus formation was significantly affected by deltamethrin; in particular, high concentrations of deltamethrin (100 and 150 µg) caused an increase in micronucleus formation at all time points. We conclude that deltamethrin affected THCs and caused the induction of micronucleus formation via genotoxic damage in G. mellonella.

Özet

In the present study, the effects of deltamethrin on cell-mediated immune response and genotoxic damage of Galleria mellonella (Lepidoptera: Pyralidae) were investigated. Different concentrations of deltamethrin (5, 20, 50, 100, and 150 µg) were administered to G. mellonella at 24, 48, 72, and 96 h. Five types of hemocytes were identified in G. mellonella: prohemocytes, plasmatocytes, granulocytes, spherulocytes, and oenocytes. Total hemocyte counts (THCs) were significantly affected by deltamethrin. At 72 h, THCs of the treated group had significantly decreased compared with the control groups at high deltamethrin concentrations (50, 100, and 150 µg); however, at 96 h, THCs had significantly increased at deltamethrin concentrations of 100 and 150 µg. Micronucleus formation was significantly affected by deltamethrin; in particular, high concentrations of deltamethrin (100 and 150 µg) caused an increase in micronucleus formation at all time points. We conclude that deltamethrin affected THCs and caused the induction of micronucleus formation via genotoxic damage in G. mellonella.

🔖

Cite This Article

KURT, D., KURT, D., KAYIŞ, T. (2015). Effects of the pyrethroid insecticide deltamethrin on the hemocytes of Galleria mellonella. Turkish Journal of Zoology, Cilt: 39 - Sayı: 3, 452–457. https://ajindex.com/articles/176739

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (14)

  1. Al-Hariri MK, Anjum S (2001). Effect of lambdacyhalothrin and deltamethrin on the haemocytes of Desert Locust, Schistocerca gregaria Forsk. Int J Agric Biol 3: 81–84.
  2. Ashhurst DE, Richards G (1964). Some histochemical observations on the blood cells of the wax moth, Galleria mellonella L. J Morph 114: 247–254.
  3. Bogdan C, Röllinghoff M, Diefenbach A (2000). Reactive oxygen and reactive nitrogen intermediates in innate and specific immunity. Curr Opin Immunol 12: 64–76.
  4. Bolognesi C, Creus A, Ostrosky-Wegman P, Marcos R (2011). Micronuclei and pesticide exposure. Mutagenesis 26: 19–26.
  5. Bronskill JK (1961). A cage to simplify the rearing of greater wax moth, Galleria mellonella (Pyralidae). J Lep Soc 15: 102–104.
  6. Büyükgüzel E, Tunaz H, Stanley D, Büyükgüzel K (2007). Eicosanoids mediate Galleria mellonella cellular immune response to viral infection. J Insect Physiol 53: 99–105.
  7. Çelik A, Mazmanci B, Çamlica Y, Aşkin A, Çömelekoğlu Ü (2005). Induction of micronuclei by lambda-cyhalothrin in Wistar rat bone marrow and gut epithelial cells. Mutagenesis 20: 125–129.
  8. Cook SM, McArthur JD (2013). Developing Galleria mellonella as a model host for human pathogens. Virulence 4: 350–353.
  9. Diaz D, Scott A, Carmichael P, Shi W, Costales C (2007). Evaluation of an automated in vitro micronucleus assay in CHO-K1 cells. Mutat Res 630: 1–13.
  10. Durmuş Y, Büyükgüzel E, Terzi B, Tunaz H, Stanley D, Büyükgüzel K (2008). Eicosanoids mediate melanotic nodulation reactions to viral infection in larvae of the parasitic wasp, Pimpla turionellae. J Insect Physiol 54: 17–24.
  11. Emre I, Kayis T, Coskun M, Dursun O, Cogun HY (2013). Changes in antioxidative enzyme activity, glycogen, lipid, protein, and malondialdehyde content in cadmium-treated Galleria mellonella larvae. Ann Entomol Soc Am 106: 371–377.
  12. Erstfeld KM (1999). Environmental fate of synthetic pyrethroids during spray drift and field run off treatments in aquatic microcosms. Chemosphere 39: 1737–1769.
  13. George PJE, Ambrose DP (2004). Impact of insecticides on the haemogram of Rhynocoris kumarii Ambrose and Livingstone (Hem. Reduviidae). J Appl Entomol 128: 600–604.
  14. Ghasemi V, Yazdi AY, Tavallaie FZ, Sendi JJ (2014). Effect of essential oils from Callistemon viminalis and Ferula gummosa on toxicity and on the hemocyte profile of Ephestia kuehniella (Lep.: Pyralidae). Arch Phytopathol Pfl 47: 268–278. Girón-Pérez MI (2010). Relationships between innate immunity in bivalve molluscs and environmental pollution. Invertebrate Surviv J 7: 1