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Journals / Turkish Journal of Zoology / 2020 / Cilt: 44 - Sayı: 2

mir-331 negatively regulates thyroglobulin secretion via ERp29

Kyoungmin MOON
Eun-ryeong LEE
Junghae KIM
Kisang KWON
O-yu KWON
Pages
189–194
Publication Date
2020-03-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Our findings may provide insight into the treatment of diseases caused by poor ER protein secretion, including ER storage diseases.

📌 3 Key Takeaways

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Objective & Hypothesis:We investigated the effect of endoplasmic reticulum ER protein 29 ERp29 on thyroglobulin Tg secretion and its negative regulation by microRNAs miRNAs .
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Key Findings & Data:ERp29 overexpression promoted Tg secretion from thyrocytes of PCCL3 cells via activation of ER stress sensors, including activation of transcription factor 6 fragmentation, XBP1 mRNA splicing by inositol-requiring enzyme 1, and phosphorylation of eukaryotic initiation factor 2 alpha as downstream actions of RNA-dependent protein kinase-like ER kinase.
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Conclusion & Impact:Our findings may provide insight into the treatment of diseases caused by poor ER protein secretion, including ER storage diseases.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:PCCL3 cells · endoplasmic reticulum stress · ERp29

Abstract

We investigated the effect of endoplasmic reticulum ER protein 29 ERp29 on thyroglobulin Tg secretion and its negative regulation by microRNAs miRNAs . ERp29 overexpression promoted Tg secretion from thyrocytes of PCCL3 cells via activation of ER stress sensors, including activation of transcription factor 6 fragmentation, XBP1 mRNA splicing by inositol-requiring enzyme 1, and phosphorylation of eukaryotic initiation factor 2 alpha as downstream actions of RNA-dependent protein kinase-like ER kinase. Thyroid hormone receptor beta is a target gene of mir-331, one of the most abundant miRNAs observed in ERp29-overexpressing cells. mir-331 negatively regulated Tg expression and secretion by ~70% compared with the control. To overcome hypothyroidism, Tg secretion at the molecular level is required to counteract negative regulation of intracellular mir-331. Our findings may provide insight into the treatment of diseases caused by poor ER protein secretion, including ER storage diseases.

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Cite This Article

MOON, K., LEE, E., KIM, J., KWON, K., KWON, O. (2020). mir-331 negatively regulates thyroglobulin secretion via ERp29. Turkish Journal of Zoology, Cilt: 44 - Sayı: 2, 189–194. https://ajindex.com/articles/176381

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