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Journals / Ege Tıp Dergisi / 2018 / Cilt: 57 - Sayı: 3

Significant increase in ZNF304 and decrease in CXCR4 gene expressions may alter anoikis in prostate cancer

ZNF304 gen ifadesinde artış ve CXCR4’de azalma ile prostat kanserinde anoikis değişebilir

ŞULE AYLA
GÜLPERİ ÖKTEM
Cüneyd PARLAYAN
Pages
157–162
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: We found that there are significant differential gene expressions in DU-145 cells which may lead to metastatic state via evasion of anoikis process.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: To analyze genes that may increase or inhibit the anoikis mechanism between prostate cancer cell line and prostate normal epithelial cell line and examine the possible role of cancer in cancer development.
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Key Findings & Data:Materials and Methods: Human prostate epithelial cell line (RWPE) and prostate cancer cell line (DU145) were acquired from ATCC.
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Conclusion & Impact:Conclusion: We found that there are significant differential gene expressions in DU-145 cells which may lead to metastatic state via evasion of anoikis process.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Aim: To analyze genes that may increase or inhibit the anoikis mechanism between prostate cancer cell line and prostate normal epithelial cell line and examine the possible role of cancer in cancer development. Materials and Methods: Human prostate epithelial cell line (RWPE) and prostate cancer cell line (DU145) were acquired from ATCC. Both cell lines were maintanied in RPMI 1640 (Biological Industries) medium. Total RNA were isolated and fragmented. Adapters were ligated to prepare RNA library for whole trasncriptome experiments. Statistics and bioinformatics analysis including mapping, clustering, sequencing were done by using Genomics Workbench v 8. (Qiagen) software. Results: As we compared the normal prostate ephitalial cells (RWPE) and prosatate cancer cells (DU 145); ZNF304,PYCARD ve NOTCH3 were significantly (p<0.05) up-regulated in DU145 cells, on the other hand, CXCR4, PAK3, SERPINB1 genes were significantly down-regulated. Conclusion: We found that there are significant differential gene expressions in DU-145 cells which may lead to metastatic state via evasion of anoikis process.

Özet

Amaç: Prostat kanser hücre hattı (DU145) ve prostat normal epitel hücre hatları (RWPE) arasında anoikis mekanizmasını arttıracak veya inhibe edebilecek genlerin analizini yapmak ve kanser gelişiminde olası rolünü incelemek. Gereç ve Yöntem: İnsan prostat epitel hücre hattı (RWPE) ve prostat kanseri hücre hatları (DU-145) Amerikan Tip Kültür Koleksiyonu (ATCC)’den temin edildi. Hücre hatlarının çoğaltılmasında ve sürdürülmesinde RPMI 1640 (Biological Industries) besi ortamı kullanıldı. Transkriptom analizi için RNA izolasyonu yapılarak, kütüphane oluşturuldu, kütüphanenin kantitasyonunun ardından NextSeq500 (illumina) ile sekanslama yapıldı. Dizileme, haritalandırma, bağıl gen ifadeleri ölçümleri gibi biyoinformatik analizler Genomics Workbench v 8 (Qiagen) yazılımı kullanılarak GRCh38 referans sekansı ile yapılmıştır. Bulgular: RWPE Normal prostat epitel hücre kültürleri ile DU145 prostat kanser hücreleri karşılaştırıldığı zaman DU-145 prostat kanser hücre kültürlerinde, ZNF304, PYCARD ve Notch3 gen expresyonlarında anlamlı bir artış (p<0,05) görülürken, CXCR4, Pak3, SerpınB1 gen ekspresyonlarında anlamlı bir azalma (p<0,05) görülmüştür. Sonuç: DU145 prostat kanseri hücre hattında anoikis ile ilişkili önemli gen ekpresyonlarında artış ve azalma gözlemledik. Değişime bağlı olarak hücrelerin anoikisden kaçarak metastatik özellik kazanabileceğini düşündük.

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Cite This Article

AYLA, Ş., ÖKTEM, G., PARLAYAN, C. (2018). Significant increase in ZNF304 and decrease in CXCR4 gene expressions may alter anoikis in prostate cancer. Ege Tıp Dergisi, Cilt: 57 - Sayı: 3, 157–162. https://ajindex.com/articles/170425

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