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Journals / Erciyes Üniversitesi Fen Bilimleri Enstitüsü Fen Bilimleri Dergisi / 2026 / Cilt: 42 Sayı: 1

Phytochemical and Bioactive Assessment of Crataegus tanacetifolia and Crataegus orientalis Fruit Extracts as a Potential Multi-Enzyme Inhibitor

Potansiyel Çoklu Enzim İnhibitörü Olarak Crataegus tanacetifolia ve Crataegus orientalis Meyve Ekstraktlarının Fitokimyasal ve Biyoaktif Değerlendirmesi

Bahar Fındık(NEVSEHIR HACI BEKTAS VELI UNIVERSITY)0000-0002-0030-4106
Hilal Yıldız(NEVSEHIR HACI BEKTAS VELI UNIVERSITY)0000-0002-7966-455X
Pages
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Publication Date
2026-03-26
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results showed that C. tanacetifolia and C. orientalis could be valuable sources of bioactive compounds for pharmaceutical and nutraceutical products.

📌 3 Key Takeaways

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Objective & Hypothesis:Plants are an endless source of bioactive compounds and have been at the centre of research investigating pivotal candidates for plant-derived drugs and dietary supplements.
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Key Findings & Data:LCMS/MS and GC-MS analyses revealed 25 phenolic compounds and two triterpenoids in C. tanacetifolia, and 26 phenolic compounds and one triterpenoid in C. orientalis, with quinic acid as the dominant phenolic compound in both extracts.
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Conclusion & Impact:The results showed that C. tanacetifolia and C. orientalis could be valuable sources of bioactive compounds for pharmaceutical and nutraceutical products.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Crataegus tanacetifolia · Crataegus orientalis · Fenolik Profil

Abstract

Plants are an endless source of bioactive compounds and have been at the centre of research investigating pivotal candidates for plant-derived drugs and dietary supplements. This study evaluated phenolic profiles, triterpenoid contents, enzyme inhibitory activities, antioxidant capacities, and total phenolic content (TPC) of the polar extracts from Crataegus tanacetifolia and Crataegus orientalis. LCMS/MS and GC-MS analyses revealed 25 phenolic compounds and two triterpenoids in C. tanacetifolia, and 26 phenolic compounds and one triterpenoid in C. orientalis, with quinic acid as the dominant phenolic compound in both extracts. Oleanolic acid and ursolic acid were detected in the C. tanacetifolia extract, whereas only ursolic acid was identified in the C. orientalis extract. Both extracts showed strong angiotensin-converting enzyme inhibition activity (~99%), indicating antihypertensive potential, as well as notable urease inhibition activity (C. tanacetifolia: 87.08%; C. orientalis: 91.66%). The C. tanacetifolia and C. orientalis extracts also exhibited significant antioxidant activity with an IC50 value of 123.38 and 97.29 µg/mL for DPPH radical scavenging activity and 82.37 and 92.34% for linoleic acid/β-carotene bleaching capacity. The TPC of the extracts was determined to be 26.13 and 76.80 mg GAE/g, respectively. The results showed that C. tanacetifolia and C. orientalis could be valuable sources of bioactive compounds for pharmaceutical and nutraceutical products.

Özet

Bitkiler, biyoaktif bileşiklerin sonsuz kaynağı olup bitki kökenli ilaçlar ve besin takviyeleri geliştirmeye yönelik araştırmaların odağında yer almaktadır. Bu çalışmada, Crataegus tanacetifolia ve Crataegus orientalis'ten elde edilen polar ekstraktların fenolik profilleri, triterpenoid içeriği, enzim inhibisyon aktiviteleri, antioksidan kapasiteleri ve toplam fenolik içerikleri (TPC) değerlendirilmiştir. LCMS/MS ve GC-MS analizleri sonucunda, C. tanacetifolia'da 25 adet fenolik bileşik ve iki adet triterpenoid, C. orientalis'te ise 26 adet fenolik bileşik ve bir adet triterpenoid tespit edilmiştir. Her iki ekstrakta da baskın fenolik bileşiğin kinik asit olduğu belirlenmiştir. C. tanacetifolia ekstraktında oleanolik asit ve ursolik asit saptanırken, C. orientalis ekstraktında yalnızca ursolik asit tespit edilmiştir. Her iki ekstrakt da güçlü anjiyotensin dönüştürücü enzim inhibisyon aktivitesi (~%99) göstererek antihipertansif potansiyeli ve ayrıca dikkate değer üreaz inhibisyon aktivitesi (C. tanacetifolia: %87,08; C. orientalis: %91,66) göstermiştir. C. tanacetifolia ve C. orientalis ekstraktları, DPPH radikal süpürme aktivitesi için sırasıyla 123,38 ve 97,29 µg/mL IC₅₀ değerleri ile linoleik asit/β-karoten ağartma testinde %82,37 ve %92,34 oranlarında önemli antioksidan aktivite göstermiştir. Ekstraktların TPC değerleri sırasıyla 26,13 ve 76,80 mg GAE/g olarak belirlenmiştir. Sonuçlar, C. tanacetifolia ve C. orientalis'in farmasötik ve nutrasötik ürünlerin geliştirilmesinde kullanılabilecek değerli biyoaktif bileşik kaynakları olabileceğini ortaya koymuştur.

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Cite This Article

Fındık, B., Yıldız, H. (2026). Phytochemical and Bioactive Assessment of Crataegus tanacetifolia and Crataegus orientalis Fruit Extracts as a Potential Multi-Enzyme Inhibitor. Erciyes Üniversitesi Fen Bilimleri Enstitüsü Fen Bilimleri Dergisi, Cilt: 42 Sayı: 1. https://doi.org/10.65520/erciyesfen.1886194

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