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Journals / Journal of Limnology and Freshwater Fisheries Research / 2026 / Cilt: 12 Sayı: 1

DNA repair mechanisms in algae

Alglerde DNA Tamir Mekanizmaları

Hatice Tunca(SAKARYA ÜNİVERSİTESİ)0000-0003-3724-5215
Rümeysa Özkılıç(SAKARYA ÜNİVERSİTESİ)0009-0002-8026-3435
Pages
13–21
Publication Date
2026-04-07
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In this study, a general overview of the repair mechanisms found in algae were presented.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:DNA repair mechanisms have been a remarkable subject in algae, as in every living organism.
📊
Key Findings & Data:Apart from this, there are limited findings in algae regarding O6 methyl guanine repair, another direct pathway.
💡
Conclusion & Impact:In this study, a general overview of the repair mechanisms found in algae were presented.
🔬Design:Derleme
📍Scope:Comprehensive Sample
⚙️Variables:fotoreaktivasyon · O-6 metil guanin onarımı · yanlış eşleşme

Abstract

DNA repair mechanisms have been a remarkable subject in algae, as in every living organism. Continuous exposure to mutagens has led to the evolution of protection mechanisms in primary producer algae of aquatic ecosystems and various pathways have developed in multiple algae. These pathways are direct and indirect. Undoubtedly, the most studied pathway is photoreactivation and photolyase enzyme. Photoreactivation is a repair mechanism that recycles thymine dimers by occuring UV radiation in both prokaryotic and eukaryotic organisms. Apart from this, there are limited findings in algae regarding O6 methyl guanine repair, another direct pathway. Mismatch repair, one of the indirect pathways, contains homologs of well-defined Echerichia coli enzymes in algae. In addition, homologs of enzymes belonging to base excision repair and nucleotide excision repair mechanisms defined in higher eukaryotes have been investigated in various algae, especially in Chlamydomonas sp. Recombinational repair is one of the indirect repair types seen in algae. In this study, a general overview of the repair mechanisms found in algae were presented.

Özet

DNA onarım mekanizmaları, her canlı organizmada olduğu gibi alglerde de dikkat çekici bir konu olmuştur. Mutajenlere sürekli maruz kalma, sucul ekosistemlerin birincil üretici alglerinde koruma mekanizmalarının evrimleşmesine yol açmış ve birçok algde çeşitli yollar gelişmiştir. Bu yollar doğrudan ve dolaylıdır. Kuşkusuz en çok çalışılan yol fotoreaktivasyon ve fotoliyaz enzimidir. Fotoreaktivasyon, hem prokaryotik hem de ökaryotik organizmalarda UV radyasyonu oluşturarak timin dimerlerini geri dönüştüren bir onarım mekanizmasıdır. Bunun dışında, alglerde bir diğer doğrudan yol olan O6 metil guanin onarımı ile ilgili sınırlı bulgular vardır. Dolaylı yollardan biri olan yanlış eşleşme onarımı, alglerde iyi tanımlanmış Echerichia coli enzimlerinin homologlarını içerir. Ayrıca, yüksek ökaryotlarda tanımlanan baz çıkarma onarımı ve nükleotid çıkarma onarımı mekanizmalarına ait enzimlerin homologları, özellikle Chlamydomonas sp.'de olmak üzere çeşitli alglerde araştırılmıştır. Rekombinasyonel onarım, alglerde görülen dolaylı onarım türlerinden biridir. Bu çalışmada, alglerde bulunan onarım mekanizmalarına genel bir bakış sunulmuştur.

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Cite This Article

Tunca, H., Özkılıç, R. (2026). DNA repair mechanisms in algae. Journal of Limnology and Freshwater Fisheries Research, Cilt: 12 Sayı: 1, 13–21. https://doi.org/10.17216/limnofish.1792319

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References (47)

  1. Amla DV. 1979. Photoreactivation of ultraviolet irradiated blue-green alga Anacystis nidulans and cyanophage AS-1. Arch Virol 59: 173-179.
  2. An M, Zheng Z, Qu C, Wang X, Chen H, Shi C, & Miao J. 2018. The first (6-4) photolyase with DNA damage repair activity from the Antarctic microalga Chlamydomonas sp. ICE-L. Mut Res./Fundam Mol Mech Mut 809: 13-19. https://doi.org/10.1016/j.mrfmmm.2018.03.004
  3. Asimgil H, Kavakli IH. 2012. Purification and characterization of five members of photolyase/cryptochrome family from Cyanidioschyzon merolae. Plant Sci 185: 190-198. https://doi.org/10.1016/j.plantsci.2011.10.005
  4. Baalen CV, & O'donnell RO. 1972. Action spectra for ultraviolet killing and photoreactivation in the blue‐green alga Agmenellum quadruplicatum. Photochem Photobiol 15(3): 269-274. https://doi.org/10.1111/j.1751-1097.1972.tb07331.x
  5. Bee L, Fabris S, Cherubini R, Mognato M, Celotti L. 2013. The efficiency of homologous recombination and non-homologous end joining systems in repairing double-strand breaks during cell cycle progression. PloS one 8(7): e69061. https://doi.org/10.1371/journal.pone.0069061
  6. Chatterjee N, Walker GC. 2017. Mechanisms of DNA damage, repair, and mutagenesis. Environ Mol Mutagen 58(5): 235-263. https://doi.org/10.1002/em.22087
  7. Compe E, Egly JM. 2016. Nucleotide excision repair and transcriptional regulation: TFIIH and beyond. Ann Rev Biochem 85(1): 265-290. https://doi.org/10.1146/annurev-biochem-060815-014857
  8. Costa RM, Chiganças V, da Silva Galhardo R, Carvalho H, & Menck CF. 2003. The eukaryotic nucleotide excision repair pathway. Biochimie 85(11): 1083-1099. https://doi.org/10.1016/j.biochi.2003.10.017
  9. Fışkın K and Özlü E. 2008. Genomun Yaşlanması: Yaşam ve Ölümle DNA’nın İkili Rolü (Yaşlanmada DNA Tamiri ve Genom Stabilitesi). Türkiye Klinikleri, J Med Scie 28(6): 16-20. (In Turkish)
  10. Fortunato AE, Annunziata R, Jaubert M, Bouly JP, & Falciatore A. 2015. Dealing with light: the widespread and multitasking cryptochrome/photolyase family in photosynthetic organisms. J Plant Physiol 172: 42-54. https://doi.org/10.1016/j.jplph.2014.06.011
  11. Friedberg EC. 2003. DNA damage and repair. Nature 421(6921): 436-440. https://doi.org/10.1038/nature01408
  12. Fromme JC, Verdine GL. 2004. Base excision repair. Adv Prot Chem 69: 1-41. https://doi.org/10.1016/S0065-3233(04)69001-2
  13. Frost BF, Small GD. 1987. The apparent lack of repair of O6-methylguanine in nuclear DNA of Chlamydomonas reinhardtii. Mut Res/Fundam Mol Mech Mutagen 181(1): 37-44.
  14. Hallmann A, Rappel A. 1999. Genetic engineering of the multicellular green alga Volvox: a modified and multiplied bacterial antibiotic resistance gene as a dominant selectable marker. The Plant J 17(1): 99-109.https://doi.org/10.1046/j.1365-13x.1999.00342.x
  15. Haney AM, Sanfilippo JE, Garczarek L, Partensky F, Kehoe DM. 2022. Multiple photolyases protect the marine cyanobacterium Synechococcus from ultraviolet radiation. Mbio 13(4): e01511-22. https://doi.org/10.1128/mbio.01511-22
  16. Hull NM, Isola MR, Petri B, Chan PS, & Linden KG. 2017 Algal DNA repair kinetics support culture-based enumeration for validation of ultraviolet disinfection ballast water treatment systems. Environ Sci Technol Lett 4(5): 192-196.
  17. Iyama T, Wilson III DM. 2013. DNA repair mechanisms in dividing and non-dividing cells. DNA repair, 12(8): 620-636. ttps://doi.org/10.1016/j.dnarep.2013.04.015
  18. Karentz D. 2015. Beyond xeroderma pigmentosum: DNA damage and repair in an ecological context. A tribute to James E. Cleaver. Photochem and Photobiol 91(2): 460-474. https://doi.org/10.1111/php.12388
  19. Kilian O, Benemann CS, Niyogi K K, & Vick B. (2011) High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp. Proceed Nat Acad Sci, 108(52): 21265-21269. https://doi.org/10.1073/pnas.1105861108
  20. Kottke T, Oldemeyer S, Wenzel S, Zou Y, Mittag M. 2017. Cryptochrome photoreceptors in green algae: unexpected versatility of mechanisms and functions. J Plant Physiol, 217: 4-14. https://doi.org/10.1016/j.jplph.2017.05.021
  21. König S, Juhas M, Jäger S, Kottke T, Büchel C. 2017. The cryptochrome—photolyase protein family in diatoms. J Plant Physiol 217: 15-19. https://doi.org/10.1016/j.jplph.2017.06.015
  22. Krejci L, Altmannova V, Spirek M, & Zhao X. 2012. Homologous recombination and its regulation. Nucl Acid Res, 40(13): 5795-5818. https://doi.org/10.1093/nar/gks270
  23. Krokan H E, Bjørås M. 2013. Base excision repair. Cold Spring Harb. Perspec Biol, 5(4): a012583. https://doi.org/10.1101/cshperspect.a012583
  24. Kumar N, Raja S, Van Houten, B. 2020. The involvement of nucleotide excision repair proteins in the removal of oxidative DNA damage. Nucl Acid Res 48(20): 11227-11243.
  25. Kurtoğlu EL, Tekedereli İ. 2015. DNA Onarım Mekanizmaları. Bal Sağ Bil Derg, 4(3): 169-177. DOI:10.5505/bsbd.2015.52523
  26. Li C, Ma L, Mou S, Wang Y, Zheng Z, Liu F,. Miao J. 2015. Cyclobutane pyrimidine dimers photolyase from extremophilic microalga: remarkable UVB resistance and efficient DNA damage repair. Mut Res/Fundam Mol Mech Mut 773: 37-42. https://doi.org/10.1016/j.mrfmmm.2014.07.010
  27. Mo J, Qi Q, Hao Y, Lei Y, Guo J. 2022. Transcriptional response of a green alga (Raphidocelis subcapitata) exposed to triclosan: photosynthetic systems and DNA repair. J Environ Sci 111: 400-411. https://doi.org/10.1016/j.jes.2021.04.023
  28. Morales-Ruiz T, Romero-Valenzuela ÁC, Vázquez‐Grande V M, Roldán-Arjona T, Ariza R R, & Córdoba‐Cañero D. 2018. Monitoring base excision repair in Chlamydomonas reinhardtii cell extracts. DNA Rep 65: 34-41. https://doi.org/10.1016/j.dnarep.2018.02.011
  29. Pascucci B, Stucki M, Jonsson ZO, Dogliotti E, Hubscher U. 1999. Long patch base excision repair with purified human proteins. DNA ligase I as patch size mediator for DNA polymerases δ and ε. J Biol Chem 274: 33696–33702. https://doi.org/10.1074/jbc.274.47.33696
  30. Pescheck F. 2019. UV-A Screening in Cladophora sp. Lowers Interenal UV-A Availability and Photoreactivation as Compared to Non-UV Screening in Ulva Intestinalis. Photochem Photobiol Sci 18: 413-423.
  31. Plecenikova A, Slaninova M, & Riha K. 2014. Characterization of DNA repair deficient strains of Chlamydomonas reinhardtii generated by insertional mutagenesis. PLoS One 9(8): e105482. https://doi.org/10.1371/journal.pone.0105482
  32. Poveda-Huertes D, Patwari P, Günther J, Fabris M, & Andersen-Ranberg J. 2023. Novel transformation strategies improve efficiency up to 10-fold in stramenopile algae. Alg Res 74: 103165. DOİ: 10.1016/j.algal.2023.103165
  33. Putnam CD. 2016. Evolution of the methyl directed mismatch repair system in Escherichia coli. DNA Rep 38:32-41. https://doi.org/10.1016/j.dnarep.2015.11.016
  34. Rajaram H, Kumar A, Kirti A, Pandey S. 2020. Double strand break (DSB) repair in Cyanobacteria: Understanding the process in an ancient organism. DNA repair, 95: 102942. https://doi.org/10.1016/j.dnarep.2020.102942
  35. Roos W P, Kaina B. 2013. DNA damage-induced cell death: from specific DNA lesions to the DNA damage response and apoptosis. Cancer Let 332(2): 237-248. https://doi.org/10.1016/j.canlet.2012.01.007 Sancar A. 1994. Structure and function of DNA photolyase. Biochem 33(1): 2-9. https://doi.org/10.1021/bi00167a001
  36. Schendel PF, Robins PE. 1978. Repair of O6-methylguanine in adapted Escherichia coli. Proceed. Nation Acad Sci 75(12): 6017-6020. https://doi.org/10.1073/pnas.75.12.6017
  37. Srivastava DK, Berg BJ, Prasad R, Molin JT, Beard WA, Tomkinson AE, Wilson SH. 1998. Mammalian abasic site base excision repair. Identification of the reaction sequence and rate-determining steps. J Biol Chem 273: 21203–21209.
  38. Sodeinde OA, Kindle KL. 1993. Homologous recombination in the nuclear genome of Chlamydomonas reinhardtii. Proceed. Nat Acad Sci 90(19): 9199-9203. https://doi.org/10.1073/pnas.90.19.9199
  39. Sonoda E, Hochegger H, Saberi A, Taniguchi Y, Takeda S. 2006. Differential usage of non-homologous end-joining and homologous recombination in double strand break repair. DNA Rep 5(9-10): 1021-1029. https://doi.org/10.1016/j.dnarep.2006.05.022
  40. Sproles AE, Berndt A, Fields FJ, Mayfield SP. 2022. Improved high-throughput screening technique to rapidly isolate Chlamydomonas transformants expressing recombinant proteins. Appl Microbiol Biotechnol 106(4): 1677-1689. https://doi.org/10.1007/s00253-022-11790-9
  41. Tillich UM, Lehmann S, Schulze K, Dühring U, Frohme M. 2012. The optimal mutagen dosage to induce point-mutations in Synechocystis sp. PCC6803 and its application to promote temperature tolerance. PLoS One 7(11): e49467. https://doi.org/10.1371/journal.pone.0049467
  42. Van Baalen C. 1968. The effects of ultraviolet irradiation on a coccoid blue-green alga: survival, photosynthesis, and photoreactivation. Plant Physiol 43(10): 1689-1695. https://doi.org/10.1104/pp.43.10.1689
  43. Vlček D, Ševčovičová A, Sviežená B, Gálová E, & Miadoková E. 2008. Chlamydomonas reinhardtii: a convenient model system for the study of DNA repair in photoautotrophic eukaryotes. Current Gen 53: 1-22. https://doi.org/10.1007/s00294-007-0163-9
  44. Wang B, Ye T, Li C, Li X, Chen L, & Wang G. 2022. Cell damage repair mechanism in a desert green algae Chlorella sp. against UV-B radiation. Ecotoxicol Environment Safe 242: 113916. https://doi.org/10.1104/pp.43.10.1689
  45. Wu Y, Zhang P, Liang Z, Liu W, Yuan Y, Liu Y,. Liu F. 2023. Genome-Wide Identification and Analysis of the Cryptochrome/Photolyase Family in the Brown Alga Saccharina japonica. J Appl Phycol 35(6): 2745-2760 https://doi.org/10.3390/plants111620
  46. Li C, Ma L, Mou S, Wang Y, Zheng Z, Liu F,. Miao J. 2015. Cyclobutane pyrimidine dimers photolyase from extremophilic microalga: remarkable UVB resistance and efficient DNA damage repair. Mut Res/Fundam Mol Mech Mut 773: 37-42. https://doi.org/10.1016/j.mrfmmm.2014.07.010
  47. Wu Y, Zhang P, Liang Z, Liu W, Yuan Y, Liu Y,. Liu F. 2023. Genome-Wide Identification and Analysis of the Cryptochrome/Photolyase Family in the Brown Alga Saccharina japonica. J Appl Phycol 35(6): 2745-2760 https://doi.org/10.3390/plants111620