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Journals / Avrasya Veteriner Bilimleri Dergisi / 2026 / Cilt: 26 Sayı: 1

Immunohistochemical investigation of the role of ubiquitin and 8-hydroxyguanosine in pathogenesis of cattle leptospirosis

Sığır leptospirozunun patogenezinde ubiquitin ve 8-hidroksiguanozin'in rollerinin immunohistokimyasal yöntemlerle araştırılması

Musa Karaman(Kafkas Üniversitesi, Veteriner Fakültesi, Patoloji AD)
Hasan Özen(Kafkas Üniversitesi, Veteriner Fakültesi, Patoloji AD)
Serpil Dağ Erginsoy(Kafkas Üniversitesi, Veteriner Fakültesi, Patoloji AD)
Mitat Şahin
Pages
33–38
Publication Date
2010-03-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: As ubiquitin and 8-OHG were shown to play roles in pathogenesis of Leptospirosis, further detailed studies to investigate and to wholly understand the mode of mechanisms of these molecules are suggested

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: This study was performed to investigate the role of ubiquitin and 8-hydroxyguanosine (8-OHG) expression in liver and kidney tissue damage in Leptospira-infected cattle.
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Key Findings & Data:Material and Method: Localization of these molecules was determined by indirect immunoperoxydase method using specific polyclonal antibodies in Leptospira positive (n=7) and healthy control animals (n=7).
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Conclusion & Impact:Conclusion: As ubiquitin and 8-OHG were shown to play roles in pathogenesis of Leptospirosis, further detailed studies to investigate and to wholly understand the mode of mechanisms of these molecules are suggested
🔬Design:Makale
📍Scope:Comprehensive Sample
⚙️Variables:Leptospiroz · ubiquitin · 8-hidroksiguanozin

Abstract

Aim: This study was performed to investigate the role of ubiquitin and 8-hydroxyguanosine (8-OHG) expression in liver and kidney tissue damage in Leptospira-infected cattle. Material and Method: Localization of these molecules was determined by indirect immunoperoxydase method using specific polyclonal antibodies in Leptospira positive (n=7) and healthy control animals (n=7). Results: A cytoplasmic immunostaining in the hepatocytes and kidney tubule epithelia against ubiquitin antibody was detected in Leptospira-infected animals. Immunostaining against 8-OHG antibody was seen intracytoplasmic and/or intranuclear in the hepatocytes, while it was mostly intracytoplasmic in kidney tubule epithelia. Conclusion: As ubiquitin and 8-OHG were shown to play roles in pathogenesis of Leptospirosis, further detailed studies to investigate and to wholly understand the mode of mechanisms of these molecules are suggested

Özet

Amaç: Bu çalışmada, Leptospira spp. ile doğal enfekte sığırların karaciğer ve böbrek dokularında oluşan hasarda ubiquitin ve 8-hidroksiguanozin’in (8-OHG) rolleri araştırılmıştır. Gereç ve Yöntem: Leptospirozlu (n=7) ve sağlıklı kontrol grubu (n=7) hayvanlarda, bu moleküllere spesifik poliklonal antikorlar kullanılarak indirekt immunoperoksidaz metodu ile antijenlerin dokulardaki lokalizasyonları belirlendi. Bulgular: Leptospirozlu hayvanların karaciğer hepatositleri ve böbrek tubul epitellerinde ubiquitin antikoruna karşı intrasitoplazmik immun boyanma tespit edildi. 8-OHG immun boyanmasının karaciğer hepatositlerinde intrasitoplazmik ve/veya intranükleer, böbrek tubul epitellerinde ise çoğunlukla intrasitoplazmik olarak lokalize olduğu gözlendi. Öneriler: Leptospirozun patogenezinde doku hasarı oluşumunda ubiquitin ile 8-OHG’nin rol oynadığı ortaya konularak, bu moleküllere ilişkin mekanizmaları daha detaylı anlayabilmek için ayrıntılı çalışmaların gerekliliği tavsiye edilmektedir.

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Cite This Article

Karaman, M., Özen, H., Erginsoy, S. D., Şahin, M. (2010). Immunohistochemical investigation of the role of ubiquitin and 8-hydroxyguanosine in pathogenesis of cattle leptospirosis. Avrasya Veteriner Bilimleri Dergisi, Cilt: 26 Sayı: 1, 33–38. https://ajindex.com/articles/1648166

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