Journals / Yenilikçi Mühendislik ve Doğa Bilimleri / 2026 / Volume: 6 Issue: 2

PLA/AgNO₃ composites fabricated by stereolithography with improved mechanical and antibacterial properties

Fatma Nur Parın(BURSA TECHNICAL UNIVERSITY)0000-0003-2048-2951
Can Ekinci(BURSA TECHNICAL UNIVERSITY)0009-0008-7295-2840
Pages
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Publication Date
2026-07-30
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

FTIR results indicated that the chemical structure of PLA was largely preserved after AgNO₃ incorporation, with minor spectral shifts suggesting possible interactions with silver-containing species.

📌 3 Key Takeaways

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Objective & Hypothesis:The development of polymer composites with antibacterial functionality and improved mechanical performance has attracted increasing attention for applications requiring both structural integrity and microbial resistance.
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Key Findings & Data:In this study, poly(lactic acid) (PLA) composites containing silver nitrate (AgNO₃; 1, 3, and 5 wt%) were fabricated by stereolithography (SLA) and characterized.
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Conclusion & Impact:FTIR results indicated that the chemical structure of PLA was largely preserved after AgNO₃ incorporation, with minor spectral shifts suggesting possible interactions with silver-containing species.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Additive manufacturing · Antibacterial composites · Stereolithography (SLA)

Abstract

The development of polymer composites with antibacterial functionality and improved mechanical performance has attracted increasing attention for applications requiring both structural integrity and microbial resistance. In this study, poly(lactic acid) (PLA) composites containing silver nitrate (AgNO₃; 1, 3, and 5 wt%) were fabricated by stereolithography (SLA) and characterized. SEM and EDX analyses confirmed the presence of silver within the PLA matrix, with relatively uniform distribution at lower contents and localized heterogeneities at higher loadings. FTIR results indicated that the chemical structure of PLA was largely preserved after AgNO₃ incorporation, with minor spectral shifts suggesting possible interactions with silver-containing species. TGA revealed a slight increase in the onset degradation temperature, from 315 °C for neat PLA to 321 °C for the composite containing 5 wt% AgNO₃. Mechanical testing showed improvements in both tensile and flexural properties with increasing AgNO₃ content, with tensile strength rising from 39.6 MPa to 55 MPa and flexural strength from 45.4 MPa to 76.7 MPa. Antibacterial tests demonstrated clear inhibition against Escherichia coli and Staphylococcus aureus, with stronger activity observed at higher AgNO₃ contents.

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Cite This Article

Taşkıngül, M. E., Parın, F. N., Ekinci, C. (2026). PLA/AgNO₃ composites fabricated by stereolithography with improved mechanical and antibacterial properties. Yenilikçi Mühendislik ve Doğa Bilimleri, Volume: 6 Issue: 2. https://doi.org/10.61112/jiens.1868301

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References (46)

  1. Gibson I, Rosen DW, Stucker B, Khorasani M (2021) Additive manufacturing technologies, 3rd ed. Springer, Cham, https://doi.org/10.1007/978-3-030-56127-7
  2. Ngo TD, Kashani A, Imbalzano G, Nguyen KTQ, Hui D (2018) Additive manufacturing (3D printing): A review of materials, methods, applications and challenges, Composites Part B: Engineering, 143:172–196, https://doi.org/10.1016/j.compositesb.2018.02.012
  3. Bártolo PJ (ed) (2011) Stereolithography: Materials, processes and applications. Springer, Boston, https://doi.org/10.1007/978-0-387-92904-0
  4. Zhou C, Chen Y, Yang Z, Khoshnevis B (2013) Digital material fabrication using mask-image-projection-based stereolithography, Rapid Prototyping Journal, 19(3):153–165, https://doi.org/10.1108/13552541311312148
  5. Melchels FPW, Feijen J, Grijpma DW (2010) A review on stereolithography and its applications in biomedical engineering, Biomaterials, 31(24):6121–6130, https://doi.org/10.1016/j.biomaterials.2010.04.050
  6. Chia HN, Wu BM (2015) Recent advances in 3D printing of biomaterials, Journal of Biological Engineering, 9: 4, https://doi.org/10.1186/s13036-015-0001-4
  7. Murphy SV, Atala A (2014) 3D bioprinting of tissues and organs, Nature Biotechnology, 32(8):773–785, https://doi.org/10.1038/nbt.2958
  8. Farah S, Anderson DG, Langer R (2016) Physical and mechanical properties of PLA, and their functions in widespread applications—A comprehensive review, Advanced Drug Delivery Reviews, 107:367–392, https://doi.org/10.1016/j.addr.2016.06.012
  9. Singhvi MS, Zinjarde SS, Gokhale DV (2019) Polylactic acid: Synthesis and biomedical applications, Journal of Applied Microbiology, 127(6):1612–1626, https://doi.org/10.1111/jam.14290
  10. Auras R, Lim LT, Selke SEM, Tsuji H (eds) (2010) Poly(lactic acid): Synthesis, structures, properties, processing, and applications. John Wiley & Sons, Hoboken, https://doi.org/10.1002/9780470649848
  11. Elsawy MA, Kim KH, Park JW, Deep A (2017) Hydrolytic degradation of polylactic acid (PLA) and its composites, Renewable and Sustainable Energy Reviews, 79:1346–1352, https://doi.org/10.1016/j.rser.2017.05.143
  12. Armentano I, Dottori M, Fortunati E, Mattioli S, Kenny JM (2010) Biodegradable polymer matrix nanocomposites for tissue engineering: A review, Polymer Degradation and Stability, 95(11):2126–2146, https://doi.org/10.1016/j.polymdegradstab.2010.06.007
  13. Fortunati E, Armentano I, Zhou Q, Puglia D, Terenzi A, Berglund LA, Kenny JM (2012) Multifunctional bionanocomposite films of poly(lactic acid), cellulose nanocrystals and silver nanoparticles, Carbohydrate Polymers, 87(2):1596–1605, https://doi.org/10.1016/j.carbpol.2011.09.066
  14. Zena Y, Periyasamy S, Tesfaye M, Tumssa Z (2025) A study on the effects of silver/zinc oxide nanocomposite dispersed modified elastin-collagen matrix on crystallization, thermal and antimicrobial properties of poly(lactic acid) films, Journal of the Taiwan Institute of Chemical Engineers, Article 106137, https://doi.org/10.1016/j.jtice.2025.106137
  15. Li J, Zhang D, Hou C (2025) Application of nano-titanium dioxide in food antibacterial packaging materials, Bioengineering, 12(1):19, https://doi.org/10.3390/bioengineering12010019
  16. Goñi-Ciaurriz L, Vélaz I (2023) Antibacterial properties of poly(lactic acid) composites for food packaging. In: Rakesh PK, Davim JP (eds) Polylactic acid composites: Sustainable biocomposites. De Gruyter, Berlin, pp 73–100, https://doi.org/10.1515/9783111067285-004
  17. Feng QL, Wu J, Chen GQ, Cui FZ, Kim TN, Kim JO (2000) A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus, Journal of Biomedical Materials Research, 52(4):662–668, https://doi.org/10.1002/1097-4636(20001215)52:4 3.0.CO;2-3
  18. Rai M, Yadav A, Gade A (2009) Silver nanoparticles as a new generation of antimicrobials, Biotechnology Advances, 27(1):76–83, https://doi.org/10.1016/j.biotechadv.2008.09.002
  19. Marambio-Jones C, Hoek EMV (2010) A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment, Journal of Nanoparticle Research, 12(5):1531–1551, https://doi.org/10.1007/s11051-010-9900-y
  20. Johnston HJ, Hutchison GR, Christensen FM, Peters S, Hankin S, Stone V (2010) A review of the in vivo and in vitro toxicity of silver and gold particulates: Particle attributes and biological mechanisms responsible for the observed toxicity, Critical Reviews in Toxicology, 40(4):328–346, https://doi.org/10.3109/10408440903453074
  21. Liau SY, Read DC, Pugh WJ, Furr JR, Russell AD (1997) Interaction of silver nitrate with readily identifiable groups: Relationship to the antibacterial action of silver ions, Letters in Applied Microbiology, 25(4):279–283, https://doi.org/10.1046/j.1472-765X.1997.00219.x
  22. Lok CN, Ho CM, Chen R, He QY, Yu WY, Sun H, Tam PKH, Chiu JF, Che CM (2007) Silver nanoparticles: Partial oxidation and antibacterial activities, Journal of Biological Inorganic Chemistry, 12(4):527–534, https://doi.org/10.1007/s00775-007-0208-z
  23. Xiu ZM, Zhang QB, Puppala HL, Colvin VL, Alvarez PJJ (2012) Negligible particle-specific antibacterial activity of silver nanoparticles, Nano Letters, 12(8):4271–4275, https://doi.org/10.1021/nl301934w
  24. Le Ouay B, Stellacci F (2015) Antibacterial activity of silver nanoparticles: A surface science insight, Nano Today, 10(3):339–354, https://doi.org/10.1016/j.nantod.2015.04.002
  25. Baykal A, Aksan OA, Oral AY, Bilge K, Kızıldağ N (2025) On the processability and antibacterial activity of silver nitrate nanocomposites manufactured by stereolithography, ACS Omega, 10(21):21391–21404, https://doi.org/10.1021/acsomega.5c00030
  26. Baykal A, Aksan OA, Oral AY, Bilge K, Kızıldağ N (2024) One-step preparation of silver nanoparticle containing polymer nanocomposites via stereolithography technique, Journal of Innovative Engineering and Natural Science, 4(2):263–275, https://doi.org/10.61112/jiens.1396859
  27. Valencia LM, Herrera M, de la Mata M, de León AS, Delgado FJ, Molina SI (2022) Synthesis of silver nanocomposites for stereolithography: In situ formation of nanoparticles, Polymers, 14(6):1168, https://doi.org/10.3390/polym14061168
  28. Aati S, Aneja S, Kassar M, Leung R, Nguyen A, Tran S, Seneviratne A, Fawzy A, Rupf S (2022) Silver-loaded mesoporous silica nanoparticles enhanced the mechanical and antimicrobial properties of 3D printed denture base resin, Journal of the Mechanical Behavior of Biomedical Materials, 134:105421, https://doi.org/10.1016/j.jmbbm.2022.105421
  29. Taormina G, Sciancalepore C, Bondioli F, Messori M (2018) Special resins for stereolithography: In situ generation of silver nanoparticles, Polymers, 10(2):212, https://doi.org/10.3390/polym10020212
  30. Rasheed M, Jawaid M, Parveez B, Bhat AH, Alamery S (2021) Morphology, structural, thermal, and tensile properties of bamboo microcrystalline cellulose/poly(lactic acid)/poly(butylene succinate) composites, Polymers, 13(3):465, https://doi.org/10.3390/polym13030465
  31. Wetzel B, Haupert F, Zhang MQ (2003) Epoxy nanocomposites with high mechanical and tribological performance, Composites Science and Technology, 63(14):2055–2067, https://doi.org/10.1016/S0266-3538(03)00115-5
  32. Quaresimin M, Schulte K, Zappalorto M, Chandrasekaran S (2016) Toughening mechanisms in polymer nanocomposites: From experiments to modelling, Composites Science and Technology, 123:187–204, https://doi.org/10.1016/j.compscitech.2015.11.027
  33. Fu SY, Feng XQ, Lauke B, Mai YW (2008) Effects of particle size, particle/matrix interface adhesion and particle loading on mechanical properties of particulate-polymer composites, Composites Part B: Engineering, 39(6):933–961, https://doi.org/10.1016/j.compositesb.2008.01.002
  34. Bikiaris ND, Koumentakou I, Samiotaki C, Meimaroglou D, Varytimidou D, Karatza A, Kourmentza C, Papageorgiou GZ (2023) Recent advances in the investigation of poly(lactic acid) (PLA) nanocomposites: Incorporation of various nanofillers and their properties and applications, Polymers, 15(5):1196, https://doi.org/10.3390/polym15051196
  35. Pušnik Črešnar K, Aulova A, Bikiaris DN, Lambropoulou D, Kuzmič K, Fras Zemljič L (2021) Incorporation of metal-based nanoadditives into the PLA matrix: Effect of surface properties on antibacterial activity and mechanical performance of PLA nanoadditive films, Molecules, 26(14):4161, https://doi.org/10.3390/molecules26144161
  36. Chieng BW, Ibrahim NA, Wan Yunus WMZ, Hussein MZ (2014) Poly(lactic acid)/poly(ethylene glycol) polymer nanocomposites: Effects of graphene nanoplatelets, Polymers, 6(1):93–104, https://doi.org/10.3390/polym6010093
  37. Kister G, Cassanas G, Vert M (1998) Effects of morphology, conformation and configuration on the IR and Raman spectra of various poly(lactic acid)s, Polymer, 39(2):267–273, https://doi.org/10.1016/S0032-3861(97)00229-2
  38. Moazzen N, Khanmohammadi M, Bagheri Garmarudi A, Kazemipour M, Ansari Dogaheh M (2019) Optimization and infrared spectrometric evaluation of the mechanical properties of PLA-based biocomposites, Journal of Macromolecular Science, Part A, 56(1):17–25, https://doi.org/10.1080/10601325.2018.1477478
  39. Carrasco F, Pagès P, Gámez-Pérez J, Santana OO, Maspoch ML (2010) Processing of poly(lactic acid): Characterization of chemical structure, thermal stability and mechanical properties, Polymer Degradation and Stability, 95(2):116–125, https://doi.org/10.1016/j.polymdegradstab.2009.11.045
  40. Ainali NM, Tarani E, Zamboulis A, Pušnik Črešnar K, Fras Zemljič L, Chrissafis K, Kostoglou M, Papageorgiou GZ, Bikiaris DN (2021) Thermal stability and decomposition mechanism of PLA nanocomposites with kraft lignin and tannin, Polymers, 13(16):2818, https://doi.org/10.3390/polym13162818
  41. Yu F, Liu T, Zhao X, Yu X, Lu A, Wang J (2012) Effects of talc on the mechanical and thermal properties of polylactide, Journal of Applied Polymer Science, 125(S2):E99–E109, https://doi.org/10.1002/app.36260
  42. Liu R, Cao J, Luo S, Wang X (2013) Effects of two types of clay on physical and mechanical properties of poly(lactic acid)/wood flour composites at various wood flour contents, Journal of Applied Polymer Science, 127(4):2566–2573, https://doi.org/10.1002/app.37797
  43. Munteanu BS, Aytac Z, Pricope GM, Uyar T, Vasile C (2014) Polylactic acid (PLA)/silver-NP/vitamin E bionanocomposite electrospun nanofibers with antibacterial and antioxidant activity, Journal of Nanoparticle Research, 16(10):2643, https://doi.org/10.1007/s11051-014-2643-4
  44. Chamakura K, Perez-Ballestero R, Luo Z, Bashir S, Liu J (2011) Comparison of bactericidal activities of silver nanoparticles with common chemical disinfectants, Colloids and Surfaces B: Biointerfaces, 84(1):88–96, https://doi.org/10.1016/j.colsurfb.2010.12.020
  45. Khina AG, Krutyakov YA (2021) Similarities and differences in the mechanism of antibacterial action of silver ions and nanoparticles, Applied Biochemistry and Microbiology, 57(6):683–693, https://doi.org/10.1134/S0003683821060053
  46. Laksemana IKA, Adi RK, Handayani M, Rahmayanti YD, Anggoro MA, Muflikhun MA, Triawan F (2025) Antibacterial enhancement and mechanical characterization of PLA resin through the addition of AgNO₃ for DLP 3D printing, Materials Letters, 399:139105, https://doi.org/10.1016/j.matlet.2025.139105