SavedAdd your journal

Journals / Recent Trends in Pharmacology / 2026 / Volume: 4 Issue: 1

Isolation, NMR Characterization, and Comparative In Vitro Antioxidant Evaluation of Resveratrol from Grape Seeds (Vitis vinifera L.)

Nisagül Kaya(Erzincan Binali Yıldırım University, Faculty of Pharmacy, Department of Pharmacognosy, Erzincan, Türkiye)0009-0003-0858-8478
Samed Şimşek(Erzincan Binali Yıldırım University, Department of Medical Services and Techniques, Erzincan, Türkiye)0000-0001-8451-3425
Sefa Gözcü(Erzincan Binali Yıldırım University, Faculty of Pharmacy, Department of Pharmacognosy, Erzincan, Türkiye)0000-0002-0735-4229
Pages
—
Publication Date
2026-04-24
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These results suggest that local grape varieties possess significant potential as natural sources for high-purity bioactive agents in the nutraceutical and pharmaceutical sectors.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: The present study aimed to isolate, characterize, and evaluate the in vitro antioxidant potential of resveratrol from grape seeds (Vitis vinifera L.) obtained from local markets in Erzincan, Türkiye.
📊
Key Findings & Data:It is hypothesized that the unique microclimate of that region enhances the bioactive profile of local grape varieties, making them a significant source of natural antioxidants.
💡
Conclusion & Impact:These results suggest that local grape varieties possess significant potential as natural sources for high-purity bioactive agents in the nutraceutical and pharmaceutical sectors.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:ABTS · DPPH · Resveratrol

Abstract

Objective: The present study aimed to isolate, characterize, and evaluate the in vitro antioxidant potential of resveratrol from grape seeds (Vitis vinifera L.) obtained from local markets in Erzincan, Türkiye. It is hypothesized that the unique microclimate of that region enhances the bioactive profile of local grape varieties, making them a significant source of natural antioxidants. Methods: Grape seeds (500 g) were extracted using 100% methanol via reflux for 4 hours (three replicates). The crude extract (30 g) underwent liquid-liquid extraction with ethyl acetate to yield a concentrated phenolic fraction (2 g). For isolation, Sephadex LH-20 adsorbent was used as the stationary phase and eluted with methanol. Structural characterization was conducted using 1H and 13C NMR spectroscopy. The antioxidant capacity of the isolated resveratrol was determined through DPPH and ABTS radical scavenging assays, comparing results with standard antioxidants (Trolox, BHA, BHT, and Ascorbic acid). Results: Chromatographic purification yielded 10 mg of pure trans-resveratrol. The structure was firmly verified by NMR spectroscopy, with the high olefinic coupling constant (J = 16.3 Hz) suggesting the trans structure. In antioxidant experiments, Trans-resveratrol demonstrated notable DPPH radical scavenging action (IC₅₀ = 17.33 ± 1.22 µg/mL). Trans-resveratrol outperformed Trolox (7.22 ± 0.24 µg/mL) and ascorbic acid (8.22 ± 0.37 µg/mL) in the ABTS test, with an IC₅₀ of 6.84 ± 0.47 µg/mL. Conclusion: The findings demonstrate that grape seeds from Erzincan markets are an excellent source of highly bioactive trans-resveratrol. The isolated compound exhibited exceptional antioxidant efficiency, particularly in the ABTS assay where it surpassed several commercial standards. These results suggest that local grape varieties possess significant potential as natural sources for high-purity bioactive agents in the nutraceutical and pharmaceutical sectors.

🔖

Cite This Article

Kaya, N., Şimşek, S., Gözcü, S. (2026). Isolation, NMR Characterization, and Comparative In Vitro Antioxidant Evaluation of Resveratrol from Grape Seeds (Vitis vinifera L.). Recent Trends in Pharmacology, Volume: 4 Issue: 1. https://doi.org/10.62425/rtpharma.1887856

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (17)

  1. Aydın, T., Çakır, A., Kazaz, C., Bayrak, N., Bayır, Y., & Taşkesenligil, Y. (2014). Insecticidal metabolites from the rhizomes of Veratrum album against adults of Colorado potato beetle, Leptinotarsa decemlineata. Chemistry & Biodiversity, 11(8), 1192–1204. https://doi.org/10.1002/cbdv.201300407
  2. Benzie, I. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry, 239(1), 70–76. https://doi.org/10.1006/abio.1996.0292
  3. Coklar, H. (2017). Antioxidant capacity and phenolic profile of berry, seed, and skin of Ekşikara (Vitis vinifera L.) grape: Influence of harvest year and altitude. International Journal of Food Properties, 20(9), 2071–2087. https://doi.org/10.1080/10942912.2016.1230870
  4. Floegel, A., Kim, D.-O., Chung, S.-J., Koo, S. I., & Chun, O. K. (2011). Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. Journal of Food Composition and Analysis, 24(7), 1043–1048. https://doi.org/10.1016/j.jfca.2011.01.008
  5. Galiniak, S., Aebisher, D., & Bartusik-Aebisher, D. (2019). Health benefits of resveratrol administration. Acta Biochimica Polonica, 66(1), 13–21. https://doi.org/10.18388/abp.2018_2749
  6. Gözcü, S., & Atmaca, M. (2025). Antioxidant potential and phenolic content of Plantago major. Current Research in Health Sciences, 2(1), 27–32. https://doi.org/10.62425/crihs.1628360
  7. Gülçin, İ. (2010). Antioxidant properties of resveratrol: A structure–activity insight. Innovative Food Science & Emerging Technologies, 11(1), 210–218. https://doi.org/10.1016/j.ifset.2009.07.002
  8. Hansen, P. E. (2021). NMR of natural products as potential drugs. Molecules, 26(12), 3763. https://doi.org/10.3390/molecules26123763
  9. Kovacic, P., & Somanathan, R. (2010). Multifaceted approach to resveratrol bioactivity: Focus on antioxidant action, cell signaling, and safety. Oxidative Medicine and Cellular Longevity, 3(2), 86–100. https://doi.org/10.4161/oxim.3.2.11147
  10. Pezzuto, J. M. (2018). Resveratrol: Twenty years of growth, development and controversy. Biomolecules & Therapeutics, 27(1), 1–14. https://doi.org/10.4062/biomolther.2018.176
  11. Pryor, W. A., Houk, K. N., Foote, C. S., Fukuto, J. M., Ignarro, L. J., Squadrito, G. L., & Davies, K. J. A. (2006). Free radical biology and medicine: It’s a gas, man! American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 291(3), R491–R511. https://doi.org/10.1152/ajpregu.00614.2005
  12. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9–10), 1231–1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  13. Sajish, M., & Schimmel, P. (2015). A human tRNA synthetase is a potent PARP1-activating effector target for resveratrol. Nature, 519(7543), 370–373. https://doi.org/10.1038/nature14028
  14. Samad, N. B., Debnath, T., Ye, M., Hasnat, M. A., & Lim, B. O. (2014). In vitro antioxidant and anti-inflammatory activities of Korean blueberry (Vaccinium corymbosum L.) extracts. Asian Pacific Journal of Tropical Biomedicine, 4(10), 807–815. https://doi.org/10.12980/APJTB.4.2014C1008
  15. Şimşek, S., Akşit, H., Köksal, E., & Altay, A. (2026). Antioxidant, antidiabetic, antiproliferative activities, and verbascoside content of four endemic Verbascum species. New Zealand Journal of Botany, 64(1), e70023. https://doi.org/10.1002/nzb2.70023
  16. Truong, V. L., Jun, M., & Jeong, W. S. (2018). Role of resveratrol in regulation of cellular defense systems against oxidative stress. BioFactors, 44(1), 36–49. https://doi.org/10.1002/biof.1399
  17. Xia, E. Q., Deng, G. F., Guo, Y. J., & Li, H. B. (2010). Biological activities of polyphenols from grapes. International Journal of Molecular Sciences, 11(2), 622–646. https://doi.org/10.3390/ijms11020622