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Journals / Sakarya University Journal of Science / 2026 / Volume: 29 Issue: 4

Responses of Helichrysum arenarium (L.) Moench Distributing in the Sivas Region to Drought Stress via the Antioxidant Defense System

Melda Şenyurt(TEKIRDAG NAMIK KEMAL UNIVERSITY)0000-0002-7486-1773
Sefer Demirbaş(TEKIRDAG NAMIK KEMAL UNIVERSITY)0000-0001-7201-3888
Pages
—
Publication Date
2025-08-31
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Germination test results showed that seeds could not germinate in 20% and 30% PEG medium and 15% PEG medium significantly inhibited growth in the early stages of seedling development.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Helichrysum species are recognized for their therapeutic properties as aromatic herbs that have been utilized for millennia in both conventional and alternative medicine.
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Key Findings & Data:Immortelle seeds grown in vitro under PEG-6000 (%0-30 PEG) were exposed to drought stress.
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Conclusion & Impact:Germination test results showed that seeds could not germinate in 20% and 30% PEG medium and 15% PEG medium significantly inhibited growth in the early stages of seedling development.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Sandy everlasting · Drought · H2O2

Abstract

Helichrysum species are recognized for their therapeutic properties as aromatic herbs that have been utilized for millennia in both conventional and alternative medicine. In the germination and early growth stages of Helichrysum arenarium (L.) Moench (immortelle) seeds obtained from the Sivas region were attempted to ascertain the morphological, physiological, and antioxidant defence system-based responses to drought stress. Immortelle seeds grown in vitro under PEG-6000 (%0-30 PEG) were exposed to drought stress. Germination test results showed that seeds could not germinate in 20% and 30% PEG medium and 15% PEG medium significantly inhibited growth in the early stages of seedling development. The physiological and biochemical responses of plants in PEG-6000 (0% (control), 5% and 10% PEG) medium during the early stages of seedling development were examined over a two-week period. The growth parameters decreased by 5% and 10% PEG medium. In addition to leaf water content (LCW), catalase (CAT) and glutathione reductase (GR) activities; APX, H2O2, SOD and TBARS values also increased. According to the seventh day data, CAT and GR activity decreased, SOD activity did not change. This observation indicates rapid responses in drought-tolerant plants such as H. arenarium, highlighting the necessity for further investigation within a seven-day period.

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Cite This Article

Şenyurt, M., Demirbaş, S. (2025). Responses of Helichrysum arenarium (L.) Moench Distributing in the Sivas Region to Drought Stress via the Antioxidant Defense System. Sakarya University Journal of Science, Volume: 29 Issue: 4. https://doi.org/10.16984/saufenbilder.1640483

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References (31)

  1. R. Perrini, I. M. Fortunato, E. Lorusso, P. Avato, “Glands, essential oils and in vitro establishment of Helichrysum italicum (Roth) G. Don ssp. microphyllum (Willd.) Nyman,” Industrial Crops and Products, vol. 29, pp. 395–403, 2009.
  2. G. Appendino, O.T. Scafati, A. Minassi, F. Pollastro, M. Ballero, A. Maxia, “Helichrysum italicum: Sleeping giant of the Mediterranean herbal medicine,” Herbalgram, vol. 105, pp. 35-45, 2015.
  3. S. Giovanelli, M. D. Leo, C. Cervelli, B. Ruffoni, D. Ciccarelli, L. Pistelli, “Essential oil composition and volatile profile of seven Helichrysum species grown in Italy,” Chemistry and Biodiversity, vol. 15, no. 5, e1700545, 2018.
  4. A. Aksoy, “Helichrysum Mill. List of plants of Turkey (vascular plants),” Nezahat Gökyiğit Botanical Garden and Flora Research Association Publication, Istanbul, pp. 163-165, 2012.
  5. M. Farooq, A. Wahid, N. Kobayashi, D. Fujita, S. M. A. Basra, “Plant drought stress: effects, mechanisms and management,” Agronomy for Sustainable Development, vol. 29, pp. 185-212, 2009.
  6. W. F. Abobatta, “Drought adaptive mechanisms of plants – a review,” Advances in Agriculture and Environmental Science, vol. 2, no. 1, pp. 42-45, 2019.
  7. Y. Fang, L. Xiong, “General mechanisms of drought response and their application in drought resistance improvement in plants,” Cellular and Molecular Life Sciences, vol. 72, pp. 673–689, 2015.
  8. J. Dumanović, E. Nepovimova, M. Natić, K. Kuča, V. Jaćević, “The significance of reactive oxygen species and antioxidant defense system in plants: A concise overview,” Frontiers in Plant Science, vol. 11, 2021 Jan 6.
  9. M. Haghpanah, S. Hashemipetroudi, A. Arzani, F. Araniti, “Drought tolerance in plants: Physiological and molecular responses,” Plants, vol. 13 no. 21, 2962, 2024.
  10. M. Hasanuzzaman, M. H. M. B. Bhuyan, F. Zulfiqar, A. Raza, S. M. Mohsin, J. A. Mahmud, M. Fujita, V. Fotopoulos, “Reactive oxygen species and antioxidant defense in plants under abiotic stress: revisiting the crucial role of a universal defense regulator,” Antioxidants, vol. 9, no. 8, 681, 2020.
  11. I. Uz, S. Savalan, “In vitro micropropagation of immortelle grass (Helichrysum italicum (Roth) G. Don),” Journal of Tekirdağ Agricultural Faculty, vol. 21, no. 1, pp. 246-255, 2024.
  12. X. Jin, C. Shi, C.Y. Yu, T. Yamada, E. J. Sacks, “Determination of leaf water content by visible and near-infrared spectrometry and multivariate calibration in miscanthus,” Frontiers in Plant Science, vol. 8, pp. 1-8, 2017.
  13. E. Bernt, H. U. Bergmeyer, “Inorganic peroxides,” in Methods of Enzymatic Analysis, H.U. Bergmeyer, Ed. Academic Press, 1974, pp. 2246-2248.
  14. K. V. Madhava Rao, T. V. S. Sresty, “Antioxidative parameters in the seedlings of pigeonpea (Cajanus cajan (L.) Millspaugh) in response to Zn and Ni stresses,” Plant Science, vol. 157, pp. 113-128, 2000.
  15. M. M. Bradford, “A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding,” Analytical Biochemistry, vol. 72, pp. 248-254, 1976.
  16. C. N. Giannopolitis, S. K. Ries, “Superoxide dismutases I. Occurrence in higher plants,” Plant Physiology, vol. 59, pp. 309-314, 1977.
  17. C. H. Foyer, B. Halliwell, “The presence of glutathione and glutathione reductase in chloroplasts: A proposed role in ascorbic acid metabolism,” Planta, vol. 133, pp. 21-25, 1976.
  18. H. U. Bergmeyer “Methoden der Enzymatischen Analyse,” 2nd Edition, Akademie Verlag, Berlin, pp. 636-647, 1970.
  19. Y. Nakano, K. Asada, “Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts,” Plant and Cell Physiology, vol. 22, pp. 867-880, 1981.
  20. M. Makena, B. Matsiliza-Mlathi, R. Kleynhans, “Seed germination and vegetative propagation of Helichrysum odoratissimum,” South African Journal of Botany, vol. 161, pp. 531-541, 2023.
  21. A. Wahab, G. Abdi, M. H. Saleem, B. Ali, S. Ullah, W. Shah, S. Mumtaz, G. Yasin, C. C. Muresan, R. A. Marc, “Plants' physio-biochemical and phyto-hormonal responses to alleviate the adverse effects of drought stress: A comprehensive review,” Plants (Basel), vol. 11, no. 13, 1620, 2022.
  22. L. J. Quan, B. Zhang, W. W. Shi, H. Y. Li, “Hydrogen peroxide in plants: A versatile molecule of the reactive oxygen species network,” Journal of Integrative Plant Biology, vol. 50, no. 1, pp. 2–18, 2008.
  23. D. Hodges, J. DeLong, C. Forney, R. K. Prange, “Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds,” Planta, vol. 207, pp. 604–611, 1999.
  24. C. Bowler, M. Van Montague, D. Inze, “Superoxide dismutase in plants,” Critical Review of Plant Science, vol. 13, pp. 199-218, 1994.
  25. B. Sade, S. Soylu, E. Yetim, “Drought and oxidative stress,” African Journal of Biotechnology, vol. 10 no. 54, pp. 11102-11109, 2011.
  26. S. Pandey, D. Fartyal, A. Agarwal, T. Shukla, D. James, T. Kaul, Y. K. Negi, S. Arora, M. K. Reddy, “Abiotic stress tolerance in plants: Myriad roles of ascorbate peroxidase,” Frontiers in Plant Science, vol. 8, pp. 1-13, 2017.
  27. G. Noctor, L. Gomez, H. Vanacker, C. H. Foyer, “Interactions between biosynthesis, compartmentation and transport in the control of glutathione homeostasis and signaling,” Journal of Experimental Botany, vol. 53, no. 372, pp. 1283-1304, 2002.
  28. G. Picciau, S. Serra, M. Porceddu, G. Bacchetta, “Seed traits and germination behaviour of four Sardinian populations of Helichrysum microphyllum subsp. tyrrhenicum (Asteraceae) along an altitudinal gradient,” Plant Biology Journal, vol. 21, pp. 498-506, 2019.
  29. I. Miclea, R. Chifor, “Germination, in vitro propagation and acclimatization in Lavandula angustifolia,” Bulletin UASVM Animal Science and Biotechnologies, vol. 75, no. 2, pp. 105-109, 2018.
  30. I. ElSayed, M. A. M. El-hamahmy, M. S. Rafudeen, A. H. Mohamed, A. A. Omar, “The impact of drought stress on antioxidant responses and accumulation of flavonolignans in milk thistle (Silybum marianum (L.) Gaertn),” Plants, vol. 8, no. 12, 611, 2019.
  31. H. Bayat, A. N. Moghadam, “Drought effects on growth, water status, proline content and antioxidant system in three Salvia nemorosa L. cultivars,” Acta Physiologiae Plantarum, vol. 41, 149, 2019.