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Journals / Sakarya University Journal of Science / 2026 / Volume: 30 Issue: 2

Investigation of Cysteine Production Capacities in Bacillus Species Isolated from Turkish Soil

Cihat Guner(ISTANBUL SABAHATTIN ZAIM UNIVERSITY)0000-0002-7935-4674
Ertan Ermiş(ISTANBUL SABAHATTIN ZAIM UNIVERSITY)0000-0002-1461-7357
Kübra Özkan Güner(YILDIZ TECHNICAL UNIVERSITY)0009-0004-7560-6717
Pages
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Publication Date
2026-04-30
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The recovery efficiency was found to be around 65% using an ion-exchange resin adsorbent.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study aimed to investigate the cysteine production capacities of microbial strains isolated from the environment (mainly soil).
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Key Findings & Data:In total, 262 bacterial colonies were isolated from 47 soil samples collected from 7 different locations.
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Conclusion & Impact:The recovery efficiency was found to be around 65% using an ion-exchange resin adsorbent.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:L-Cysteine production · Bacillus sp. · Fermentation optimization

Abstract

This study aimed to investigate the cysteine production capacities of microbial strains isolated from the environment (mainly soil). In total, 262 bacterial colonies were isolated from 47 soil samples collected from 7 different locations. Isolated bacterial colonies were first grown in tryptic soy broth to determine their cysteine production capacity. The amounts of cysteine produced by isolated bacteria varied from 0.3 to 2.0 g.L-1. Five out of 262 isolates produced a significant amount of cysteine. Based on 16S rRNA gene sequence analysis using the BLAST (basic local alignment search tool) database., the highest cysteine-producing strain was identified as Bacillus sp. strain CG1. Cysteine production potential was evaluated by adjusting the growth medium composition as well as incubations conditions. Sixteen different chemical compositions of growth media were evaluated. The incubation parameters adjusted to produce cysteine were temperature, pH, time, and agitation speed. The highest cysteine yield was 4.24 ± 0.47 g.L-1 under growth conditions of pH 7.5, 175 rpm, and 40 ℃ at 84 h. The recovery efficiency was found to be around 65% using an ion-exchange resin adsorbent.

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Cite This Article

Guner, C., Ermiş, E., Güner, K. Ö. (2026). Investigation of Cysteine Production Capacities in Bacillus Species Isolated from Turkish Soil. Sakarya University Journal of Science, Volume: 30 Issue: 2. https://doi.org/10.16984/saufenbilder.1823176

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