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Journals / Uluslararası Gıda Tarım ve Hayvan Bilimleri Dergisi / 2026 / Cilt: 6 Sayı: 2

Development of Pink Tomato Breeding Materials Resistant to TYLCV and FORL Pathogens

TYLCV ve FORL Hastalık Etmenlerine Karşı Dayanıklılık Pembe Domates Islah Materyallerinin Geliştirilmesi

Hüseyin Basım(AKDENİZ ÜNİVERSİTESİ)0000-0002-8059-3680
Osman Kandil0000-0003-3806-423X
Cansu Ökem0009-0007-5634-8830
Pages
100–110
Publication Date
2026-09-29
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These findings demonstrate the effectiveness of marker-assisted selection for the rapid identification of disease resistance genes and provide valuable genetic resources for pyramiding multiple resistance genes, accelerating tomato breeding, and supporting the development of sustainable, disease-resistant tomato cultivars.

📌 3 Key Takeaways

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Objective & Hypothesis:Abstract Tomato Yellow Leaf Curl Virus (TYLCV) and Fusarium oxysporum f.sp. radicis-lycopersici (FORL) are among the most destructive pathogens limiting tomato (Solanum lycopersicum L.) production worldwide, causing substantial yield and quality losses.
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Key Findings & Data:The codominant Ty3-P6-25 marker was used to detect the Ty-3 gene associated with TYLCV resistance, whereas the C2-25 CAPS marker was employed to identify the Frl gene conferring resistance to FORL.
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Conclusion & Impact:These findings demonstrate the effectiveness of marker-assisted selection for the rapid identification of disease resistance genes and provide valuable genetic resources for pyramiding multiple resistance genes, accelerating tomato breeding, and supporting the development of sustainable, disease-resistant tomato cultivars.
🔬Design:Araştırma Makalesi
📍Scope:33 samples
⚙️Variables:Domates · TYLCV · FORL

Abstract

Abstract Tomato Yellow Leaf Curl Virus (TYLCV) and Fusarium oxysporum f.sp. radicis-lycopersici (FORL) are among the most destructive pathogens limiting tomato (Solanum lycopersicum L.) production worldwide, causing substantial yield and quality losses. The development of cultivars with durable and multiple disease resistance has therefore become a major objective of modern tomato breeding. In this study, molecular markers were used to identify resistance genes against TYLCV and FORL in pink tomato breeding lines developed within the Istanbul Tarım A.Ş. tomato breeding program. Breeding materials previously characterized for resistance to root-knot nematodes (Meloidogyne spp.) and Tomato Spotted Wilt Virus (TSWV), together with additional breeding lines, were evaluated to identify genotypes carrying multiple disease resistance genes. The codominant Ty3-P6-25 marker was used to detect the Ty-3 gene associated with TYLCV resistance, whereas the C2-25 CAPS marker was employed to identify the Frl gene conferring resistance to FORL. Among the 167 breeding lines analyzed with the Ty3-P6-25 marker, 13 (9.7%) were homozygous resistant, 5 (3.7%) were heterozygous resistant, and 116 (86.6%) were homozygous susceptible, while 33 samples produced no interpretable results. Analysis of 177 breeding lines with the C2-25 marker identified 3 (2.5%) homozygous resistant, 10 (8.2%) heterozygous resistant, and 109 (89.3%) homozygous susceptible genotypes, whereas 55 samples yielded no evaluable results. The molecular analyses confirmed the presence of resistance alleles to both TYLCV and FORL within the breeding population and enabled the identification of elite breeding lines carrying resistance to multiple economically important pathogens when integrated with previous marker data for Mi-1 and Sw-5. These findings demonstrate the effectiveness of marker-assisted selection for the rapid identification of disease resistance genes and provide valuable genetic resources for pyramiding multiple resistance genes, accelerating tomato breeding, and supporting the development of sustainable, disease-resistant tomato cultivars.

Özet

Öz Domates Sarı Yaprak Kıvırcıklık Virüsü (TYLCV) ve Fusarium oxysporum f.sp. radicis-lycopersici (FORL), dünya genelinde domates (Solanum lycopersicum L.) üretimini sınırlayan ve önemli verim ile kalite kayıplarına yol açan en yıkıcı patojenler arasındadır. Bu nedenle, çoklu hastalık dayanıklılığına sahip çeşitlerin geliştirilmesi, modern domates ıslahının temel bir hedefi haline gelmiştir. Bu çalışmada, İstanbul Tarım A.Ş. domates ıslah programı kapsamında geliştirilen pembe domates ıslah hatlarında TYLCV ve FORL'ye karşı dayanıklılık genlerini belirlemek amacıyla moleküler belirteçler kullanılmıştır. Çoklu hastalık dayanıklılık genlerini taşıyan genotipleri tespit etmek amacıyla, daha önce kök ur nematodları (Meloidogyne spp.) ve Domates Lekeli Solgunluk Virüsü'ne (TSWV) karşı dayanıklılık bakımından karakterize edilmiş ıslah materyalleri ve ilave ıslah hatları değerlendirilmiştir. TYLCV dayanıklılığı ile ilişkili Ty-3 genini saptamak için kodominant Ty3-P6-25 belirteci kullanılırken, FORL'ye karşı dayanıklılık sağlayan Frl genini belirlemek için C2-25 CAPS belirteci kullanılmıştır. Ty3-P6-25 belirteci ile analiz edilen 167 ıslah hattından 13'ü (%9,7) homozigot dayanıklı, 5'i (%3,7) heterozigot dayanıklı ve 116'sı (%86,6) homozigot duyarlı olarak belirlenmiş; 33 örnekten ise yorumlanabilir sonuç alınamamıştır. C2-25 belirteci ile yapılan analizlerde 177 ıslah hattından 3'ünün (%2,5) homozigot dayanıklı, 10'unun (%8,2) heterozigot dayanıklı ve 109'unun (%89,3) homozigot duyarlı genotipe sahip olduğu tespit edilmiş; 55 örnekten ise değerlendirilebilir sonuç elde edilememiştir. Moleküler analizler, ıslah popülasyonunda hem TYLCV hem de FORL'ye karşı direnç alellerinin varlığını doğrulamış; Mi-1 ve Sw-5 için elde edilen önceki belirteç verileriyle entegre edildiğinde, ekonomik açıdan önemli birden fazla patojene karşı dayanıklılık taşıyan seçkin ıslah hatlarının belirlenmesine olanak tanımıştır. Bu bulgular, hastalık dayanıklılık genlerinin hızlı bir şekilde tespit edilmesinde belirteç destekli seçimin etkinliğini ortaya koymakta; ayrıca çoklu dayanıklılık genlerinin bir araya getirilmesi (piramitleme), domates ıslahının hızlandırılması ve sürdürülebilir, hastalığa dayanıklı domates çeşitlerinin geliştirilmesinin desteklenmesi adına değerli genetik kaynaklar sağlamaktadır.

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Cite This Article

Basım, H., Kandil, O., Ökem, C. (2026). Development of Pink Tomato Breeding Materials Resistant to TYLCV and FORL Pathogens. Uluslararası Gıda Tarım ve Hayvan Bilimleri Dergisi, Cilt: 6 Sayı: 2, 100–110. https://ajindex.com/articles/1623430

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