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Journals / Journal of Agricultural Biotechnology / 2026 / Cilt: 7 Sayı: 1

Antagonistic effects of Trichoderma spp. isolated from Düzce agricultural soils on Fusarium oxysporum f. sp. lycopersici, the causative agent of tomato root and crown rot, under in vitro conditions

Düzce tarım topraklarından izole edilen Trichoderma türlerinin, in vitro koşullar altında domates kök ve Kökboğazı çürüklüğüne neden olan Fusarium oxysporum f. sp. lycopersici'ye karşı antagonistik etkileri

Sema Nur Buldu(DÜZCE ÜNİVERSİTESİ)0009-0008-2991-1736
İsmet Yıldırım(Düzce Üniversitesi)0000-0002-0473-1332
Pages
1–9
Publication Date
2026-07-03
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In the study, it was concluded that GYO1F1 and GY1B1-0 isolates, identified as T. virens by macroscopic and microscopic morphological identification, could be promising biological control agents against FOL.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, the antagonistic activities of Trichoderma spp. isolated from Düzce agricultural soils against Fusarium oxysporum f. sp. lycopersici (FOL), which causes collar and root rot of tomato, were investigated under in vitro conditions.
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Key Findings & Data:In a quadruplicate culture test of antagonist candidate Trichoderma isolates isolated from 120 soil samples taken from agricultural fields, 10 isolates were determined to have antagonistic activity against FOL In dual culture tests, while isolates GYO1F1 and GY1B1-0 showed the highest antagonistic effect against mycelial growth of the FOL at 100% and 71.87%, respectively, followed by isolates GY1F1-0, GY2F2-0, KTM1-0, GK2F2-1, GY12F2-0, and GYO2F2-0 with antagonistic effects ranging from 10.9% to 31.06%.
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Conclusion & Impact:In the study, it was concluded that GYO1F1 and GY1B1-0 isolates, identified as T. virens by macroscopic and microscopic morphological identification, could be promising biological control agents against FOL.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Domates · Fusarium oxysporum f. sp. lycopersici · Trichoderma spp.

Abstract

In this study, the antagonistic activities of Trichoderma spp. isolated from Düzce agricultural soils against Fusarium oxysporum f. sp. lycopersici (FOL), which causes collar and root rot of tomato, were investigated under in vitro conditions. In a quadruplicate culture test of antagonist candidate Trichoderma isolates isolated from 120 soil samples taken from agricultural fields, 10 isolates were determined to have antagonistic activity against FOL In dual culture tests, while isolates GYO1F1 and GY1B1-0 showed the highest antagonistic effect against mycelial growth of the FOL at 100% and 71.87%, respectively, followed by isolates GY1F1-0, GY2F2-0, KTM1-0, GK2F2-1, GY12F2-0, and GYO2F2-0 with antagonistic effects ranging from 10.9% to 31.06%. However, isolate KT1M1-2 exhibited the lowest antagonistic activity (3.26%). The volatile compounds of isolates GYO1F1 and GY1B1-0 inhibited the colonial growth of FOL by 13.37% and 38.45%, respectively, on the sixth day after inoculation using the dual Petri dish method. In the study, it was concluded that GYO1F1 and GY1B1-0 isolates, identified as T. virens by macroscopic and microscopic morphological identification, could be promising biological control agents against FOL.

Özet

Bu çalışmada Düzce tarım topraklarından izole edilen Trichoderma spp.’nin domateste kök ve kök çürüklüğüne neden olan Fusarium oxysporum f. sp. lycopersici (FOL)’ye antagonistik etkileri in vitro koşullarda araştırılmıştır. Tarım alanlarından alınan 120 toprak örneğinden izole edilen antagonist adayı Trichoderma izolatlarının FOL’ye karşı dörtlü kültür denemelerinde, 10 izolatın antagonistik etki gösterdiği tespit edilmiştir. Bu izolatlarla ve FOL ile ikili kültür denemelerinde GYO1F1 ve GY1B1-0 izolatları patojenin miselyal gelişmesine karşı en yüksek antagonistik etkiyi (sırasıyla %100 ve %71.87) gösterirken, bunları %10.9-%31.06 arasında antagonistik etkiyle GY1F1-0, GY2F2-0, KTM1-0, GK2F2-1, GY12F2-0, GYO2F2-0 izolatları izlemiştir. KT1M1-2 izolatı ise en düşük antagonistik etkiyi (%3.26) göstermiştir. GYO1F1 ve GY1B1-0 izolatlarının uçucu yağları ikili petri yönteminde FOL’nin koloniyal gelişimini inokulasyondan sonra altıncı günde sırasıyla ortalama %13.37 ve %38.45 oranında inhibe ettikleri tespit edilmiştir. Çalışmada izolatlarının makroskopik ve mikroskopik morfolojik tanılarında T. virens oldukları tespit edilen GYO1F1 ve GY1B1-0 izolatları FOL’e karşı biyolojik mücadelede ümitvar oldukları sonucuna varılmıştır.

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Cite This Article

Buldu, S. N., Yıldırım, İ. (2026). Antagonistic effects of Trichoderma spp. isolated from Düzce agricultural soils on Fusarium oxysporum f. sp. lycopersici, the causative agent of tomato root and crown rot, under in vitro conditions. Journal of Agricultural Biotechnology, Cilt: 7 Sayı: 1, 1–9. https://doi.org/10.58728/joinabt.1897740

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