Journals / ANKEM Dergisi / 2011 / Cilt: 25 - Sayı: 3

The frequency of metallo-beta-lactamase production in pseudomonas spp. strains isolated from intensive care unit

Yoğun bakımda izole edilen pseudomonas spp. suşlarında metallo-beta-laktamaz sıklığının araştırılması

Pages
150–156
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Consequently, detecting MBL production in Pseudomonas strains by phenotypical methods will provide guidance for treatment of infections and may be helpful for infection control by early initiate relevant control measures.

📌 3 Key Takeaways

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Objective & Hypothesis:In the present study, we evaluated the frequency of metallo-beta-lactamase (MBL) production in 43 multidrug resistant Pseudomonas spp. strains isolated from intensive care unit of our hospital during June and December 2008.
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Key Findings & Data:Thirty four and sixteen out of 43 strains were found MBL positive by IMP-EDTA and by modified Hodge test, respectively.
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Conclusion & Impact:Consequently, detecting MBL production in Pseudomonas strains by phenotypical methods will provide guidance for treatment of infections and may be helpful for infection control by early initiate relevant control measures.
🔬Design:Research Article
📍Scope:2008 Verileri
⚙️Variables:Domain Parameters

Abstract

In the present study, we evaluated the frequency of metallo-beta-lactamase (MBL) production in 43 multidrug resistant Pseudomonas spp. strains isolated from intensive care unit of our hospital during June and December 2008. The MBL production was examined by modified Hodge test and combined disc (IMP- EDTA) test. Thirty four and sixteen out of 43 strains were found MBL positive by IMP-EDTA and by modified Hodge test, respectively. Consequently, detecting MBL production in Pseudomonas strains by phenotypical methods will provide guidance for treatment of infections and may be helpful for infection control by early initiate relevant control measures.

Özet

Haziran-Aralık 2008 arasında hastanemiz yoğun bakım ünitesinde hastalardan alınan kültür örneklerinden infeksiyon etkeni olarak izole edilen çoğul dirençli 43 Pseudomonas spp. suşunda metallo-beta-laktamaz (MBL) üretimi, modifiye Hodge testi ve kombine disk (IPM-EDTA) yöntemi ile araştırılmıştır. Kombine disk (IPM-EDTA) yöntemi ile 43 suşun 34’ünde, modifiye Hodge testi ile 43 suşun 16’sında MBL üretimi tespit edilmiştir. Hastanelerde, fenotipik yöntemlerle Pseudomonas suşlarında MBL üretiminin tespit edilmesi, hem bu etkenlerle oluşan infeksiyonların tedavisinde yol gösterici olacak hem de erken başlatılacak kontrol önlemleriyle infeksiyon kontrolüne katkı sağlayacaktır.

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Cite This Article

DEMİRDAĞ, K., ÇABALAK, M., ÖZGÜLER, M. (2011). The frequency of metallo-beta-lactamase production in pseudomonas spp. strains isolated from intensive care unit. ANKEM Dergisi, Cilt: 25 - Sayı: 3, 150–156. https://ajindex.com/articles/161445

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References (3)

  1. Altoparlak U, Aktas F, Celebi D, Ozkurt Z, Akcay MN. Prevalence of metallo-b-lactamases among Pseudomonas aeruginosa and Acinetobacter baumannii isolated from burn wounds and in vitro activities of antibiotic combinations against these isolates, Burns 2005;31(6):707-10. http://dx.doi.org/10.1016/j.burns.2005.02.017 PMid:16129224 2. Ambler RP. The structure of beta-lactamases, p.321-31, Philos Trans R Soc London Ser B (London) (1980). 3. Behera B, Mathur P, Das A, Kapil A, Sharma V. An evaluation of four different phenotypic techniques for detection of metallo-beta-lactamase producing Pseudomonas aeruginosa, Indian J Med Microbiol 2008;26(3):233-7. http://dx.doi.org/10.4103/0255-0857.39587 PMid:18695320 4. Blondell-Hill E, Henry DA, Speert DP: Pseudomonas, “Murray PR, Baron EJ, Jorgensen JH, Landry ML, Pfaller MA (eds). Manual of Clinical Microbiology, 9th ed., Vol 1” kitabında s.734-48,
  2. ASM Press,
  3. Washington, DC (2007). 5. Bush KG, Jacoby GA, Medeiros AA. A functional classification scheme for beta-lactamases and its correlation with molecular structures, Antimicrob Agents Chemother 1995;39(6):1211-33. PMid:7574506 PMCid:162717 6. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Fifteenth Informational Supplement, CLSI document M100-S15, CLSI, Wayne, PA (2005). 7. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Nineteenth Informational Supplement, CLSI document M100-S19, CLSI, Wayne, PA (2009). 8. Falagas ME, Karageorgopoulos DE. Pandrug resistance (PDR), extensive drug resistance (XDR), and multidrug resistance (MDR) among Gram-negative bacilli: need for international harmonization in terminology, Clin Infect Dis 2008;46(7):1121-2. http://dx.doi.org/10.1086/528867 PMid:18444833 9. Gayyurhan E, Zer Y, Mehli M, Akgün S. Yoğun bakım Ünitesi hastalarından izole edilen Pseudomonas aeruginosa suşlarının antibiyotik duyarlılıkları ve metallo-beta-laktamaz oranlarının belirlenmesi, İnfeksiyon Derg 2008;22(1):49-52. 10. Ito H, Arakawa Y, Ohsuka S, Wacharotayankun R, Kato N, Ohta M. Plasmid-mediated dissemination of the metallo-b-lactamase gene blaIMP among clinical isolated strains of Serratia marcescens, Antimicrob Agents Chemother 1995;39(4):824-9. PMid:7785978 PMCid:162636 11. Jack GW, Richmond MH. A comparative study of eight distinct beta-lactamases synthesized by gram-negative bacteria, J Gen Microbiol 1970;61(1): 43-61. PMid:5489064 12