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Journals / Experimental Biomedical Research / 2019 / Cilt: 2 - Sayı: 3

Effects of magnesium sulphate on liver ischemia/reperfusion injury in a rat model

Kerem AKKOCA
Hamit YOLDAS
Mustafa SİT
Ibrahim KARAGOZ
Isa YILDIZ
Abdullah DEMİRHAN
Murat BİLGİ
Ozgur Mehmet YİS
Aysel KUKNER
Ayhan CETİNKAYA
Oguz CATAL
Bahri OZER
Pages
93–102
DOI
—
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⚡ TL;DR(Too Long; Didn't Read)

Conclusion: The main finding of the present study suggested that magnesium sulphate pretreatment moderately decreased the liver damage through its anti-inflammatory and anti-oxidant effects in a rat model of liver I/R.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: To investigate the protective efficacy of magnesium sulphate in a model of rat liver ischemiareperfusion (I/R) injury.
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Key Findings & Data:Method: 32 adult female Wistar-Albino rats (250 to 350 g) were used in this experimental study.
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Conclusion & Impact:Conclusion: The main finding of the present study suggested that magnesium sulphate pretreatment moderately decreased the liver damage through its anti-inflammatory and anti-oxidant effects in a rat model of liver I/R.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Özet

Aim: To investigate the protective efficacy of magnesium sulphate in a model of rat liver ischemiareperfusion (I/R) injury. Method: 32 adult female Wistar-Albino rats (250 to 350 g) were used in this experimental study. Rats were divided into 4 groups according to liver ischemia and magnesium sulfate application methods. Group 1 (C); control, group 2 (M); magnesium sulphate, group 3 (I/R); liver I/R, group 4 (I/R+M); I/R + magnesium sulphate treated. The blood samples were centrifuged for the study of aspartate aminotransferase (AST), alanine aminotransferase, prothrombin time (PT), international normalized ratio (INR) troponin I, total antioxidant status (TAS), total oxidant status (TOS) assays. The livers of the animals were removed at the end of the study and samples were taken for histopathological examination. Results: AST and INR values were significantly decreased in I/R+M group compared to I/R group. There was no significant difference in ALT values of the groups. Although not statistically significant, the TAS values were increased in I/R + M group compared to I/R group rats. In addition, the value of TOS was found to be lower in I/R + M group rats. In the histopathological examination, the mean values of apoptosis and necrosis were lower in the IR+M group compared to the I/R group. Conclusion: The main finding of the present study suggested that magnesium sulphate pretreatment moderately decreased the liver damage through its anti-inflammatory and anti-oxidant effects in a rat model of liver I/R.

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Cite This Article

AKKOCA, K., YOLDAS, H., SİT, M., KARAGOZ, I., YILDIZ, I., DEMİRHAN, A., BİLGİ, M., YİS, O. M., KUKNER, A., CETİNKAYA, A., CATAL, O., OZER, B. (2019). Effects of magnesium sulphate on liver ischemia/reperfusion injury in a rat model. Experimental Biomedical Research, Cilt: 2 - Sayı: 3, 93–102. https://ajindex.com/articles/160325

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References (13)

  1. Ugras D, Mustafa U, Oguzhan O, Mehmet Y, Seckin A, Orhan GO, et al. Protective effects of escin on hepatic ischemia/reperfusion injury in rats Acta Medica Mediterr. 2016: 32(4):973-79. [2]Weigand K, Brost S, Steinebrunner N, Büchler M, Schemmer P, Müller M. Ischemia/Reperfusion injury in liver surgery and transplantation: pathophysiology. HPB Surg. 2012
  2. 176723. [3] Erturk E. Ischemia-reperfusion injury and volatile anesthetics. Biomed Res Int. 2014
  3. 526301. [4] Girgin S, Gedik E, Taçyildiz IH. [Evaluation of surgical methods in patients with blunt liver trauma]. Ulus Travma Acil Cerrahi Derg. 2006
  4. 12(1):35-42. [5] de Araujo M, Andrade L, Coimbra TM, Rodrigues AC Jr, Seguro AC. Magnesium supplementation combined with Nacetylcysteine protects against postischemic acute renal failure. J Am Soc Nephrol. 2005
  5. 16(11):3339-49. [6] Akan M, Ozbilgin S, Boztas N, Celik A, Ozkardesler S, Ergur B, et al. Effect of magnesium sulfate on renal ischemiareperfusion injury in streptozotocininduced diabetic rats. Eur Rev Med Pharmacol Sci. 2016
  6. 20(8):1642-55. [7] Kaptanoglu AF, Arca T, Kilinc K. Magnesium sulfate protects fetal skin from intrauterine ischemia reperfusion injury. Arch Dermatol Res. 2012
  7. 304(7):529-32. [8] Kao M-C, Jan W-C, Tsai P-S, Wang T-Y, Huang C-J. Magnesium sulfate mitigates lung injury induced by bilateral lower limb ischemia-reperfusion in rats. J Surg Res. 2011
  8. 171(1):e97-e106. [9]Lin C, Tsai P, Hung Y, Huang C. L-type calcium channels are involved in mediating the anti-inflammatory effects of magnesium sulphate. Br J Anaesth. 2009
  9. 104(1):44-51. [10]Abu-Amara M, Gurusamy K, Hori S, Glantzounis G, Fuller B, Davidson BR. Systematic review of randomized controlled trials of pharmacological interventions to reduce ischaemiareperfusion injury in elective liver resection with vascular occlusion. HPB (Oxford). 2010
  10. 12(1):4-14. [11]Nakajima Y, Shimamura T, Kamiyama T, Matsushita M, Sato N, Todo S. Control of intraoperative bleeding during liver resection: analysis of a questionnaire sent to 231 Japanese hospitals. Surg Today. 2002
  11. 32(1):48-52. [12]van der Bilt JD, Livestro DP, Borren A, van Hillegersberg R, Rinkes IB. European survey on the application of vascular clamping in liver surgery. Dig Surg. 2007
  12. 24(6):423-35. [13]Gurusamy KS, Kumar Y, Pamecha V, Sharma D, Davidson BR. Ischaemic preconditioning for elective liver resections performed under vascular occlusion. Cochrane Database Syst Rev. 2009
  13. :CD007629. [14]Dufour DR, Lott JA, Nolte FS