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Journals / Alatarım / 2004 / Cilt: 3 - Sayı: 1

The optimisation of regeneration in cucumber (Cucumis sativus L)

Hıyar (Cucumis sativus L)' da rejenerasyonunun optimizasyonu

Yalçın Yeşim MENDİ
Serbest Sinem KOBANER
A. Fikret FIRAT
Journal
Alatarım
Pages
1–4
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

However higher AVG concentration (1 mg/1) reduced regeneration ratio.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study is to optimize the rejeneration protocol in cucumber using different concentration of BA and AVG.
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Key Findings & Data:In regeneration medium, MS medium containing 0.0, 0.5 and 1.0 mg/1 AVG, 0.0, 0.5, 1.0 and 2.0 mg/1 BA and 3 mg/1 phytagel were used.
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Conclusion & Impact:However higher AVG concentration (1 mg/1) reduced regeneration ratio.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:hıyar · etilen · AVG

Abstract

The aim of this study is to optimize the rejeneration protocol in cucumber using different concentration of BA and AVG. In regeneration medium, MS medium containing 0.0, 0.5 and 1.0 mg/1 AVG, 0.0, 0.5, 1.0 and 2.0 mg/1 BA and 3 mg/1 phytagel were used. In this study, flamingo bill explant type was used. The highest regeneration ratio (87.5 %) was obtained from MS medium containing 0.5 mg/1 AVG and 0.5 mg/1 BA. Regeneration capacity was induced by addition of AVG. However higher AVG concentration (1 mg/1) reduced regeneration ratio.

Özet

Çalışmanın amacı, hıyar'da farklı BA ve AVG konsantrasyonlarını deneyerek etkili rejenerasyon metodunu optimize etmektir. Rejenerasyon ortamı olarak AVG'nin 0.0, 0.5 ve 1.0 mg/1, BA'nin 0.0, 0.5, 1.0 ve 2.0 mg/1 konsantrasyonlarını içeren MS (Makro, Mikro ve Vitamin) ve katılaştırıcı olarak da Phytagel (3 g/l) kullanılmıştır. Denemede 'flamingo bili' eksplant tipi denenmiştir. Denemenin sonucunda, en yüksek rejenerasyon oranı, 0.5 mg/1 AVG ve 0.5 mg/1 BA ilave edilmiş MS ortamından %87.5 oranında elde edilmiştir. Ortama AVG'nin eklenmesi ile rejenerasyon oranı artmıştır. Ancak, AVG'nin yüksek konsantrasyonunda (lmg/1) rejenerasyon oranınınm düştüğü gözlenmiştir.

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Cite This Article

MENDİ, Y. Y., KOBANER, S. S., FIRAT, A. F. (2004). The optimisation of regeneration in cucumber (Cucumis sativus L). Alatarım, Cilt: 3 - Sayı: 1, 1–4. https://ajindex.com/articles/158213

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