Journals / ANKEM Dergisi / 2011 / Cilt: 25 - Sayı: 4

Microbiological assesment of bile samples of patients who had cholecystectomy

Kolesistektomi yapılan hastaların safra örneklerinin mikrobiyolojik değerlendirilmesi

Pages
227–231
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The evaluation of the bile culture and starting the appropriate antibiotic according to the isolated agent will shorten the treatment duration.

📌 3 Key Takeaways

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Objective & Hypothesis:Cholecystectomy is one of the most common gastrointestinal operations performed in general surgical units.
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Key Findings & Data:Of 108 bile samples examined, 36 (33 %) were culture positive. 26 (72 %) of which were Gram negative bacteria (12 Escherichia coli, 8 Klebsiella pneumoniae, 4 Pseudomonas aeruginosa, 2 Enterobacter cloacae); 8 (22 %) were Gram positive cocci (4 coagulase negative staphylococci, 2 Enterococcus faecalis, 2 Staphylococcus aureus) and 2 (6 %) were anaerobic bac- teria (Bacteroides fragilis).
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Conclusion & Impact:The evaluation of the bile culture and starting the appropriate antibiotic according to the isolated agent will shorten the treatment duration.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Cholecystectomy is one of the most common gastrointestinal operations performed in general surgical units. The aim of this study was to determine the microorganisms and their antibiotic susceptibilities that were isolated from bile samples of patients who had cholecystectomy because of acute or chronic cholecystitis. The identification and antibiotic susceptibility of the isolated aerobic bacteria were performed by Phoenix (Becton Dickinson Diagnostic Instrument Systems, Spark, Md, USA) automated system and the identification of anaerobic bacteria was performed by mini API (bioMerioux-France) system. Of 108 bile samples examined, 36 (33 %) were culture positive. 26 (72 %) of which were Gram negative bacteria (12 Escherichia coli, 8 Klebsiella pneumoniae, 4 Pseudomonas aeruginosa, 2 Enterobacter cloacae); 8 (22 %) were Gram positive cocci (4 coagulase negative staphylococci, 2 Enterococcus faecalis, 2 Staphylococcus aureus) and 2 (6 %) were anaerobic bac- teria (Bacteroides fragilis). No fungi was isolated and there was no mixed infection. The most effective antibiotics against Gram negative and Gram positive bacteria were amikacin (100 %) and vankomycin (100 %), respectively. The microbiologic examination of the samples obtained during cholecystectomy, facilitates the diagnosis and treatment of post-operative infections. The evaluation of the bile culture and starting the appropriate antibiotic according to the isolated agent will shorten the treatment duration.

Özet

Kolesistektomi, genel cerrahi kliniklerinde uygulanan en yaygın gastrointestinal operasyonlardan biridir. Bu çalışmada akut veya kronik kolesistit nedeniyle kolesistektomi yapılan hastalardan alınan safra örneklerinde üreyen mikroorganizmaların saptanması ve antibiyotik duyarlılıklarının belirlenmesi amaçlanmıştır. Üreyen aerob bakterilerin identifikasyonu ve antibiyo tik duyarlılıkları Phoenix (Becton Dickinson Diagnostic Instrument Systems, Spark, Md, USA) otomatize sisteminde; anae rob bakterilerin identifikasyonu ise mini API (bioMerioux-Fransa) sisteminde gerçekleştirilmiştir. İncelenen 108 safra örneğinin 36’sında (% 33) üreme saptanmıştır. Bu 36 örneğin 26’sında (% 72) Gram negatif çomaklar (12 Escherichia coli, 8 Klebsiella pneumoniae, 4 Pseudomonas aeruginosa, 2 Enterobacter cloacae); 8’inde (% 22) Gram pozitif koklar (4 koagülaz negatif stafilokok, 2 Enterococcus faecalis, 2 Staphylococcus aureus) ve 2’sinde (% 6) anaerob bakteri (Bacteroides fragilis) üremiştir. Hiçbir örnekte mantar ürememiş ve miks infeksiyon görülmemiştir. İzole edilen Gram negatif bakterilere karşı amikasin (% 100), Gram pozitif bakterilere karşı ise vankomisin (% 100) en etkili antibiyotikler olarak bulunmuştur. Kolesistektomi sırasında alınan örneklerin mikrobiyolojik incelenmesi, post-operatif gelişen infeksiyonların tanı ve tedavisini kolaylaştırmaktadır. Safra kültürünün değerlendirilmesi ve izole edilen etkene uygun antibiyotiğin başlanması tedavi süresini kısaltacaktır.

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Cite This Article

NERGİZ, Ş., AKPOLAT, N., GÜL, K. (2011). Microbiological assesment of bile samples of patients who had cholecystectomy. ANKEM Dergisi, Cilt: 25 - Sayı: 4, 227–231. https://ajindex.com/articles/157490

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