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Journals / Turkish Medical Student Journal / 2020 / Cilt: 7 Sayı: 3

THE EFFECT OF 5-FU AND RUXOLITINIB ON MITOCHONDRIAL APOPTOSIS IN GLIOBLASTOMA U87 CELL LINE

Gonca Aksu(TRAKYA ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0002-9250-012X
Oğuzhan Doğanlar(TRAKYA ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, TIBBİ BİYOLOJİ ANABİLİM DALI)0000-0003-2654-7269
Zeynep Banu Doğanlar(TRAKYA ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, TIBBİ BİYOLOJİ ANABİLİM DALI)0000-0002-1365-9897
Pages
130–139
Publication Date
2020-10-31
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Therefore, further studies are needed.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Aims: The aim of this study is to carry out the effect of 5-Fluorouracil alone or combined with Ruxolitinib on both apoptosis and JAK/STAT pathway in U87 glioblastoma cells.
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Key Findings & Data:Methods: We used U87 glioblastoma cell lines as the human brain cancer cells.
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Conclusion & Impact:Therefore, further studies are needed.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:5-Fluorouracil · Ruxolitinib · glioblastoma

Abstract

Aims: The aim of this study is to carry out the effect of 5-Fluorouracil alone or combined with Ruxolitinib on both apoptosis and JAK/STAT pathway in U87 glioblastoma cells. Methods: We used U87 glioblastoma cell lines as the human brain cancer cells. We treated the cells with 5-Fluorouracil (3.125 μM-400 μM) alone and with a combination of Ruxolitinib (100 μM or 400 μM of Ruxolitinib with 3.125-25 μM 5-Fluorouracil), and performed the MTT test for calculating IC50 value. Molecular fluorescence staining was performed with Hoechst and acridine orange/ethidium bromide probes. The alteration in mitochondrial apoptosis and JAK/STAT pathways to drug treatment was analyzed by the qRT-PCR assay. Results: Decrease in cell viability was more prominent in U87 cells treated with a combination of 5-Fluorouracil and Ruxolitinib compared to those treated with 5-Fluoro- uracil alone. In gene expression analysis, apoptosis signals were observed in cells treated with 5-Fluorouracil alone and 5-Fluo- rouracil+Ruxolitinib treatment. Conclusion: Treatment with 5-Fluorouracil alone and 5-Fluorouracil+Ruxolitinib combination increased apoptosis in U87 glioblastoma cells. However, it is difficult to mention an evident difference between treatments. Therefore, further studies are needed.

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Cite This Article

Aksu, G., Doğanlar, O., Doğanlar, Z. B. (2020). THE EFFECT OF 5-FU AND RUXOLITINIB ON MITOCHONDRIAL APOPTOSIS IN GLIOBLASTOMA U87 CELL LINE. Turkish Medical Student Journal, Cilt: 7 Sayı: 3, 130–139. https://ajindex.com/articles/1558221

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