Under the optimal culture conditions, the maximum cell growth and lipase activity were determined as 2.219 0D 600nm and 1607 U/L, respectively.
📌 3 Key Takeaways
🎯
Objective & Hypothesis:Lipases are widely used in textile, food, medical and chemical industries.
📊
Key Findings & Data:The present study was performed to produce lipase from thermophilic bacterial strains using waste frying oil as substrate.
💡
Conclusion & Impact:Under the optimal culture conditions, the maximum cell growth and lipase activity were determined as 2.219 0D 600nm and 1607 U/L, respectively.
Lipases are widely used in textile, food, medical and chemical industries. The present study was performed to produce lipase from thermophilic bacterial strains using waste frying oil as substrate. Among four bacterial strains, Bacillus licheniformis A7 (GenBank accesion number: KC310458 ) was determined to be the best lipase producer. A frying oil concentration of 40 mL/L, temperature of 55 °C, initial pH of 6.0 and incubation time of 72 h were found to be optimal for lipase production from Bacillus sp. Under the optimal culture conditions, the maximum cell growth and lipase activity were determined as 2.219 0D 600nm and 1607 U/L, respectively.
Baltacı, M. Ö., Tuysuz, E., Ozkan, H., Taskın, M., Adıguzel, A. (2019). Lipase production from thermophilic bacteria using waste frying oil as substrate. Teknik Bilimler Dergisi, Cilt: 9 Sayı: 3 Özel Sayı, 23–27. https://doi.org/10.35354/tbed.510140
P. Anbu, S.C. Gopinath, A.C. Cihan and B. P. Chaulagain, “Microbial enzymes and their applications in industries and medicine,” BioMed research international, 2013.
F. Hasan, A. A. Shah and A. Hameed, “Industrial applications of microbial lipases,” Enzyme and Microbial technology, vol. 39(2), pp. 235-251, 2006.
N. A. Soliman,, M. Knoll, Y. R.Abdel-Fattah, R.D. Schmid and S. Lange, “Molecular cloning and characterization of thermostable esterase and lipase from Geobacillus thermoleovorans YN isolated from desert soil in Egypt,” Process Biochemistry, vol. 42(7), pp. 1090-1100, 2007.
B. Van Den Burg, “Extremophiles as a source for novel enzymes,” Current opinion in microbiology, vol. 6(3), pp. 213-218, 2003.
T.S. Mohan, A. Palavesam and G. Immanvel, “Isolation and characterization of lipase-producing Bacillus strains from oil mill waste.” African journal of Biotechnology, vol. 7(15), 2008.
B. Venkatesagowda, E. Ponugupaty, A. M. Barbosa and R.F. Dekker, “Solid-state fermentation of coconut kernel-cake as substrate for the production of lipases by the coconut kernel-associated fungus Lasiodiplodia theobromae VBE-1,” Annals of microbiology, vol. 65(1), pp. 129-142, 2015.
A. Kumar and S.S. Kanwar, “Lipase production in solid-state fermentation (SSF): recent developments and biotechnological applications,” Dynamic Biochemistry, Process Biotechnology and Molecular Biology, vol. 6(1), pp. 13-27, 2012.
D. K. Parihar, “Production of lipase utilizing linseed oilcake as fermentation substrate,” Int. J. Sci. Environ. Technol, vol. 1(3), pp. 135-143, 2012.
H. Alkan, Z. Baysal, F. Uyar and M. Dogru, “Production of lipase by a newly isolated Bacillus coagulans under solid-state fermentation using melon wastes,” Applied biochemistry and biotechnology, vol. 136(2), pp. 183-192, 2007.
A. N. Ferreira, D. D. S. Ribeiro, R. A. Santana, A. C. Santos Felix, L. D. G. Alvarez, E. D. O. Lima et al., “ Production of lipase from Penicillium sp. using waste oils and Nopalea cochenillifera,” Chem. Engi. Commun., vol. 204, pp. 1167-1173, 2017.
L. P. Christopher, V. P. Zambare, A. Zambare, H. Kumard and L. Malek, “A thermo-alkaline lipase from a new thermophile Geobacillus thermodenitrificans AV-5 with potential application in biodiesel production,” vol. 90(11), pp. 2007-2016, J. Chem. Technol. Biotechnol, 2015.
D. Yanmis, M. O. Baltaci, M. Gulluce and A. Adiguzel, “ Identification of thermophilic strains from geothermal areas in Turkey by using conventional and molecular techniques,” Res J Biotechnol, vol. 10(1), pp. 39-45, 2015.
M. O. Baltaci, B. Genc, S. Arslan, G. Adiguzel and A. Adiguzel, “Isolation and characterization of thermophilic bacteria from geothermal areas in Turkey and preliminary research on biotechnologically important enzyme production,” Geomicrobiology journal, vol. 34(1), pp. 53-62, 2017.
T. C. Hung, R. Giridhar, S. H. Chiou, W.T. Wu,“Binary immobilization of Candida rugosa lipase on chitosan,” J. Mol. Catal. B – Enzym, vol. 26, pp. 69–78 2003.
M. Taskin and S. Erdal, “Production of carotenoids by Rhodotorula glutinis MT‐5 in submerged fermentation using the extract from waste loquat kernels as substrate,” Journal of the Science of Food and Agriculture, vol. 91(8), pp. 1440-1445, 2011.
M. C. T. Damaso, M.A. Passianoto, S.C. Freitas, R. C. A. Lago and S. Couri, S., “Utilization of agroindustrial residues for lipase production by solid-state fermentation,” Braz. J. Microbiol, vol. 39(4), pp. 66–70, 2008.
L. Bora and M. Bora, “Optimization of extracellular thermophilic highly alkaline lipase from thermophilic Bacillus sp isolated from Hotspring of Arunachal Pradesh, India,” Brazilian Journal of Microbiology, vol. 43(1), pp. 30-42, 2012.
N. K. Saun, P. Mehta and R. Gupta, “Purification and physicochemical properties of lipase from thermophilic Bacillus aerius,” Journal of oleo science, vol. 63(12), pp. 1261-1268, 2014.
S. Dharmsthiti and S. Luchai, “Production, purification and characterization of thermophilic lipase from Bacillus sp. THL027,” FEMS microbiology letters, vol. 179(2), pp. 241-246, 1999.
M. Guncheva, D. Zhiryakova, “Catalytic properties and potential applications of Bacillus lipases,” J. Mol. Catal. B: Enzym, vol. 68, pp. 1–21, 2011.
T. C. Hung, R. Giridhar, S. H. Chiou, W.T. Wu,“Binary immobilization of Candida rugosa lipase on chitosan,” J. Mol. Catal. B – Enzym, vol. 26, pp. 69–78 2003.