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Journals / Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2026 / Cilt: 9 Sayı: 2

Protective Effects of Resveratrol and Berberine Against Oxidative Stress Induced by Imidacloprid in Rat Blood and Spleen Tissues

İmidakloprid’in Sıçan Kan ve Dalak Dokularında Neden Olduğu Oksidatif Strese Karşı Resveratrol ve Berberin’in Koruyucu Etkileri

Hatice Karaboduk(Gazi üniversitesi)0000-0001-6752-7219
Çağlar Adıgüzel(GAZİ ÜNİVERSİTESİ)0000-0003-3716-0051
Fatma Gökçe Apaydın(GAZİ ÜNİVERSİTESİ)0000-0002-2771-7488
Yusuf Kalender(GAZİ ÜNİVERSİTESİ)0000-0001-5457-0517
Pages
946–959
Publication Date
2026-03-16
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These results show that RES and BBR supplementation significantly reduced the oxidative damage caused by IMI in blood and spleen tissue.

📌 3 Key Takeaways

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Objective & Hypothesis:Imidacloprid (IMI) is one of the most commonly used neonicotinoid insecticides in the world for agricultural control.
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Key Findings & Data:In the study, rats were divided into 7 groups with 6 animals in each group. 1st group: control, 2nd group: RES (20 mg/kg v.a.), 3rd group: BBR (100 mg/kg v.a.), 4th group: IMI (9 mg/kg v.a.), 5th group: IMI+RES, 6th group: IMI+BBR, 7th group: IMI+RES+BBR.
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Conclusion & Impact:These results show that RES and BBR supplementation significantly reduced the oxidative damage caused by IMI in blood and spleen tissue.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:İmidakloprid · Resveratrol · Berberin

Abstract

Imidacloprid (IMI) is one of the most commonly used neonicotinoid insecticides in the world for agricultural control. As a result of its overuse, serious public health and environmental problems are emerging. Resveratrol (RES) and berberine (BBR) are natural compounds with antioxidant, anti-inflammatory and anticancer properties. The aim of this study was to investigate the protective effect of RES and BBR against oxidative damage caused by IMI on blood and spleen tissue in rats. In the study, rats were divided into 7 groups with 6 animals in each group. 1st group: control, 2nd group: RES (20 mg/kg v.a.), 3rd group: BBR (100 mg/kg v.a.), 4th group: IMI (9 mg/kg v.a.), 5th group: IMI+RES, 6th group: IMI+BBR, 7th group: IMI+RES+BBR. The administration substances were given to the rats by oral gavage for 28 days. A significant increase in MDA levels in the blood and spleen tissues of rats in the IMI group was found compared to the control group (p<0.05), and a significant decrease in the activities of antioxidant enzymes SOD, CAT, GPx and GST (p<0.05). When the RES and BBR groups were compared with the IMI group, a significant decrease in MDA levels and a significant increase in antioxidant enzyme activities were observed (p<0.05). These results show that RES and BBR supplementation significantly reduced the oxidative damage caused by IMI in blood and spleen tissue.

Özet

İmidakloprid (IMI) tarımsal mücadelede dünyada en sık kullanılan neonikotinoid insektisitlerden biridir. Aşırı kullanımının sonucu olarak ciddi bir halk sağlığı problemleri ve çevre sorunları ortaya çıkmaktadır. Resveratrol (RES) ve berberin (BBR) antioksidan, antiinflamatuvar ve antikanser gibi özellikleri olan doğal bileşiklerdir. Bu çalışmanın amacı sıçanlarda IMI’nın kan ve dalak dokusu üzerine neden olduğu oksidatif hasara karşı RES ve BBR’nin koruyucu etkisini araştırmaktır. Çalışmada sıçanlar her grupta 6 hayvan olacak şekilde 7 gruba ayrılmıştır. 1. grup: kontrol, 2. grup: RES (20 mg/kg v.a.), 3. grup: BBR (100 mg/kg v.a.), 4. grup: IMI (9 mg/kg v.a.), 5. grup: IMI+RES, 6. grup: IMI+BBR, 7. grup: IMI+RES+BBR. Uygulama maddeleri sıçanlara 28 gün boyunca oral gavaj yolu ile verilmiştir. IMI grubundaki sıçanların kan ve dalak dokularındaki MDA düzeylerinde kontrol grubuna göre anlamlı bir artış (p<0,05), antioksidan enzimler SOD, CAT, GPx ve GST aktivitelerinde ise anlamlı bir azalma (p<0,05) gözlenmiştir. RES ve BBR grupları IMI grubuyla karşılaştırıldığında MDA düzeylerinde anlamlı azalma, antioksidan enzim aktivitelerinde ise anlamlı artış gözlendi (p<0,05). Bu sonuçlar göstermektedir ki IMI’nın kan ve dalak dokusunda oluşturduğu oksidatif hasara karşı, RES ve BBR takviyesinin önemli ölçüde bu toksisiteyi azalttığı tespit edilmiştir.

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Cite This Article

Karaboduk, H., Adıgüzel, Ç., Apaydın, F. G., Kalender, Y. (2026). Protective Effects of Resveratrol and Berberine Against Oxidative Stress Induced by Imidacloprid in Rat Blood and Spleen Tissues. Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 9 Sayı: 2, 946–959. https://doi.org/10.47495/okufbed.1738508

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References (57)

  1. Abdelgawad FE., Abd El-Rahman GI., Behairy A., Abd-Elhakim YM., Saber TM., Metwally MM., Abd El-Fatah SS., Samaha MM., Saber T., Aglan MA. Thymol's modulation of cellular macromolecules, oxidative stress, DNA damage, and NF-kB/caspase-3 signaling in the liver of imidacloprid-exposed rats. Environmental Toxicology and Pharmacology 2024; 109: 104492. https://doi.org/10.1016/j.etap.2024.104492
  2. Abdel-kawy SH., Mohamed DH., Lotfy M., Mogahed OE. Possible hepatoprotective role of berberine versus silymarin on methotrexate toxicity: Histological and biochemical study. Egyptian Journal of Histology 2023; 46(1): 435-447.
  3. Adiguzel C., Karaboduk H., Uzunhisarcikli M. Protective role of melatonin against abamectin-induced biochemical, immunohistochemical, and ultrastructural alterations in the testicular tissues of rats. Microscopy and Microanalysis 2024; 30(5): 962-977. https://doi.org/10.1093/mam/ozae080
  4. Aebi H. Catalase in vitro. Methods in Enzymology 1984; 105: 121-126. https://doi.org/10.1016/S0076-6879(84)05016-3
  5. Akash MSH., Akbar M., Rehman K., Shah MA., Panichayupakaranant P., Imran M., Assiri MA. Biochemical profiling of berberine-enriched extract in aluminum chloride induced oxidative damage and neuroinflammation. Environmental Science and Pollution Research 2023; 30(36): 85263-85275.
  6. Akbar M., Shabbir A., Rehman K., Akash MSH., Shah MA. Neuroprotective potential of berberine in modulating Alzheimer's disease via multiple signaling pathways. Journal of Food Biochemistry 2021; 45(10): e13936. https://doi.org/10.1111/jfbc.13936
  7. Akbel E., Arslan-Acaroz D., Demirel HH., Kucukkurt I., Inced S. The subchronic exposure to malathion, an organophosphate pesticide, causes lipid peroxidation, oxidative stress, and tissue damage in rats: the protective role of resveratrol. Toxicology Research 2018; 7: 503. https://doi.org/10.1039/C8TX00030A
  8. Alagal RI., AlFaris NA., Alshammari GM., AL Tamimi JZ., AlMousa LA., Yahya MA. The protection afforded by berberine against chemotherapy‐mediated nephropathy in rats involves regulation of the antioxidant axis. Basic & Clinical Pharmacology & Toxicology 2023; 132(1): 98-110.
  9. AlBasher G., Abdel-Daim MM., Almeer R., Ibrahim KA., Hamza RZ., Bungau S., Aleya L. Synergistic antioxidant effects of resveratrol and curcumin against fipronil-triggered oxidative damage in male albino rats. Environmental Science and Pollution Research 2020; 27: 6505-6514. https://doi.org/10.1007/s11356-019-07344-8
  10. Apaydın FG., Pandır D., Kalender S., Baş H., Kalender Y. Hematoprotective effect of vitamins C and E against subchronic toxicity of bendiocarb: Biochemical evidences. Journal of Food Biochemistry 2018; 42(6): e12659. https://doi.org/10.1111/jfbc.12659
  11. Apaydın F., Baş H., Kalender Y. Lead and cadmium induced oxidative stress in the epididymis and spleen of rats: effects of sesamol. Commagene Journal of Biology 2021; 5(1): 7-11. https://doi.org/10.31594/commagene.797945
  12. Apaydin FG., Kalender S., Bas H., Demir F., Kalender Y. Lead nitrate induced testicular toxicity in diabetic and non-diabetic rats: Protective role of sodium selenite. Brazilian Archives of Biology and Technology 2015; 58: 68-74. https://doi.org/10.1590/S1516-8913201400025
  13. Baş H., Apaydin FG., Kalender S., Aydoğdu G., Adigüzel Ç., Taştan H., Kalender Y. Dimethoate-induced oxidative stress and DNA damage in rat blood cells: Prevenive effects of ferulic acid. Turkish Bulletin of Hygiene and Experimental Biology 2022; 79(2): 243-254. https://doi.org/10.5505/TurkHijyen.2022.09734
  14. Baş H., Apaydın FG., Kalender S., Kalender Y. Lead nitrate and cadmium chloride induced hepatotoxicity and nephrotoxicity: Protective effects of sesamol on biochemical indices and pathological changes. Journal of Food Biochemistry 2021; 45(7): e13769. https://doi.org/10.1111/jfbc.13769
  15. Bas H., Kalender Y. Chlorpyrifos induced cardiotoxicity in rats and the protective role of quercetin and catechin. Gazi University Journal of Science 2011; 24(3): 387-395.
  16. Binmahfouz LS., Hassanein EH., Bagher AM., Hareeri RH., Alamri ZZ., Algandaby MM., Abdel-Daim MM., Abdel-Naim AB. Berberine alleviates chlorpyrifos-induced nephrotoxicity in rats via modulation of Nrf2/HO-1 axis. Heliyon 2024; 10(3). https://doi.org/10.1016/j.heliyon.2024.e25233
  17. Bojarski B., Witeska M. Blood biomarkers of herbicide, insecticide, and fungicide toxicity to fish-a review. Environmental Science and Pollution Research 2020; 27: 19236-19250. https://doi.org/10.1007/s11356-020-08248-8
  18. Chupradit S., Bokov D., Zamanian MY., Heidari M., Hakimizadeh E. Hepatoprotective and therapeutic effects of resveratrol: A focus on anti‐inflammatory and antioxidative activities. Fundamental & Clinical Pharmacology 2022; 36(3): 468-485. https://doi.org/10.1111/fcp.12746
  19. Drabkin DL. Spectrophotometric studies: XIV. The crystallographic and optical properties of the hemoglobin of man in comparison with those of other species. Journal of Biological Chemistry 1946; 164(2): 703-723.
  20. Goudarzi M., Kalantar M., Malayeri A., Basir Z., Karamallah MH., Kalantar H. Berberine alleviates sodium arsenite-induced renal and liver toxicity by regulating oxidative stress and inflammation in rats. Toxicology and Environmental Health Sciences 2023; 15(2): 157-172. https://doi.org/10.1007/s13530-023-00168-7
  21. Habig WH., Pabst MJ., Jakoby WB. Glutathione S-transferases: the first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry 1974; 249(22): 7130-7139. https://doi.org/10.1016/S0021-9258(19)42083-8
  22. Hafez Hafez M., El-Kazaz SES., El-Neweshy MS., Shukry M., Ghamry HI., Tohamy HG. Resveratrol mitigates heat stress-induced testicular injury in rats: Enhancing male fertility via antioxidant, antiapoptotic, pro-proliferative, and anti-inflammatory mechanisms. Naunyn-Schmiedeberg's Archives of Pharmacology 2025; 1-15. https://doi.org/10.1007/s00210-024-03759-4
  23. Ibrahim KA., Abdelgaid HA., Eleyan M., Mohamed RA., Gamil NM. Resveratrol alleviates cardiac apoptosis following exposure to fenitrothion by modulating the sirtuin1/c-Jun N-terminal kinases/p53 pathway through pro-oxidant and inflammatory response improvements: In vivo and in silico studies. Life Sciences 2022; 290: 120265. https://doi.org/10.1016/j.lfs.2021.120265
  24. Jado MA., Kalender Y. Small intestinal toxicity induced by imidacloprid in rats and the protective role of berberine and resveratrol. Bozok Journal of Science 2024; 2(2): 38-46. https://doi.org/10.70500/bjs.1498720
  25. Jakubczyk K., Skonieczna-Żydecka K., Kałduńska J., Stachowska E., Gutowska I., Janda K. Effects of resveratrol supplementation in patients with non-alcoholic fatty liver disease a meta analysis. Nutrients 2020; 12(8): 2435. https://doi.org/10.3390/nu12082435
  26. Juan CA., Pérez de la Lastra JM., Plou FJ., Pérez-Lebeña E. The chemistry of reactive oxygen species (ROS) revisited: outlining their role in biological macromolecules (DNA, lipids and proteins) and induced pathologies. International Journal of Molecular Sciences 2021; 22(9): 4642. https://doi.org/10.3390/ijms22094642
  27. Kalender S., Apaydin FG., Baş H., Kalender Y. Protective effects of sodium selenite on lead nitrate-induced hepatotoxicity in diabetic and non-diabetic rats. Environmental Toxicology and Pharmacology 2015; 40(2): 568-574. https://doi.org/10.1016/j.etap.2015.08.011
  28. Kalender S., Apaydin FG., Kalender Y. Testicular toxicity of orally administrated bisphenol A in rats and protective role of taurine and curcumin. Pakistan Journal of Pharmaceutical Sciences 2019; 32(3).
  29. Karaboduk H., Adıgüzel Ç., Apaydın FG., Kalender S., Uzunhisarcikli M., Kalender Y. Fenamifos' un sıçan kan ve dalak dokusunda sebep olduğu oksidatif stres üzerine naringenin'in koruyucu rolü. Journal of the Institute of Science and Technology 2024a; 14(2): 625-635. https://doi.org/10.21597/jist.1381156
  30. Karaboduk H., Adiguzel C., Apaydin FG., Uzunhisarcikli M., Kalender S., Kalender Y. The ameliorative effect of Naringenin on fenamiphos induced hepatotoxicity and nephrotoxicity in a rat model: Oxidative stress, inflammatory markers, biochemical, histopathological, immunohistochemical and electron microscopy study. Food and Chemical Toxicology 2024b; 192: 114911. https://doi.org/10.1016/j.fct.2024.114911
  31. Karaboduk H., Uzunhisarcikli M., Kalender Y. Protective effects of sodium selenite and vitamin E on mercuric chloride-induced cardiotoxicity in male rats. Brazilian Archives of Biology and Technology 2015; 58(2): 229-238. https://doi.org/10.1590/S1516-8913201400339
  32. Li J., Li J., Zhai L., Lu K. Co-exposure of polycarbonate microplastics aggravated the toxic effects of imidacloprid on the liver and gut microbiota in mice. Environmental Toxicology and Pharmacology 2023; 101: 104194. https://doi.org/10.1016/j.etap.2023.104194
  33. Liang Y., Dong B., Pang N., Hu J. Abamectin induces cytotoxicity via the ROS, JNK, and ATM/ATR pathways. Environmental Science and Pollution Research 2020; 27: 13726-13734. https://doi.org/10.1007/s11356-019-06869-2
  34. Liu L., Shi M., Wu Y., Hao J., Guo J., Li S., Dai P., Gao J. Protective effects of resveratrol on honeybee health: Mitigating pesticide-induced oxidative stress and enhancing detoxification. Pesticide Biochemistry and Physiology 2025; 210: 106403. https://doi.org/10.1016/j.pestbp.2025.106403
  35. Ma P., Li J., Huang Q., Wei S., Ge H., Wang Z. Exploring the mechanism of anti-fatigue of resveratrol based on network pharmacology and molecular docking, and in vitro studies. Scientific Reports 2023; 13(1): 2894. https://doi.org/10.1038/s41598-023-30141-w
  36. Marklund S., Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry 1974; 47(3): 469-474. https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  37. Mikolić A., Karačonji IB. Imidacloprid as reproductive toxicant and endocrine disruptor: investigations in laboratory animals. ArhivZaHigijenu Rada I Toksikologiju 2018; 69(2): 103-108. https://doi.org/10.2478/aiht-2018-69-3144
  38. Moghaddam HK., Baluchnejadmojarad T., Roghani M., Khaksari M., Norouzi P., Ahooie M., Mahboobi F. Berberine ameliorate oxidative stres and astrogliosis in the hippocampus of STZ-induced diabetic rats. Molecular Neurobiology 2014; 49: 820-826.
  39. Monfared AL. Protective role of Vitisvinifera L extract against imidacloprid-intoxicated kidney, liver, and testis in Wistar rats. Comparative Clinical Pathology 2024; 33(1): 135-144. https://doi.org/10.1007/s00580-023-03533-7
  40. Moyano P., Flores A., San Juan J., García J., Anadón MJ., Plaza JC., Naval MV., Fern'andez MC., Guerra-Men'endez L., Del Pino J. Imidacloprid unique and repeated treatment produces cholinergic transmission disruption and apoptotic cell death in SN56 cells. Food and Chemical Toxicology 2024; 193: 114988. https://doi.org/10.1016/j.fct.2024.114988
  41. Mudgal R., Sharma S., Singh S., Ravichandiran V. The neuroprotective effect of ascorbic acid against imidacloprid-induced neurotoxicity and the role of HO-1 in mice. Frontiers in Neurology 2023; 14: 1130575. https://doi.org/10.3389/fneur.2023.1130575
  42. Nema M., Dutta BJ., Singh S. Alpha-Lipoic acid alleviates imidacloprid-induced neuro-behavioral deficits in rats via Nrf2/HO-1 pathway. Toxicology Mechanisms and Methods 2024; 34(2): 176-188. https://doi.org/10.1080/15376516.2023.2266027
  43. Ohkawa H., Ohishi N., Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry 1979; 95(2): 351-358. https://doi.org/10.1016/0003-2697(79)90738-3
  44. Paglia DE., Valentine WN. Studies on the quantative and qualitative characterization of glutathione peroxidase. Journal of Laboratory and Clinical Medicine 1987; 70: 158-165.
  45. Pei H., He Z., Du R., Han C., Sheng Y., Wang J., Zhou X., Li W., Cao C., Sheng J., Wang X. Imidacloprid activates Kupffer cells pyroptosis to induce liver injury in mice via P2X7. International Immunopharmacology 2023; 119: 110179. https://doi.org/10.1016/j.intimp.2023.110179
  46. Peng Y., Zheng X., Zhang S., Luo Z., Song L., Chen H., Yao X. Advances in the activity of resveratrol and its derivatives in cardiovascular diseases. Archiv der Pharmazie 2025; 358(2): e2400865. https://doi.org/10.1002/ardp.202400865
  47. Poliserpi MB., Noya Abad T., De Gerónimo E., Aparicio V., Brodeur JC. Behavioral and physiological response of the passerine bird Agelaioidesbadius to seeds coated with imidacloprid. Environmental Science and Pollution Research 2023; 30(33): 80293-80310. https://doi.org/10.1007/s11356-023-28074-y
  48. Saber TM., Arisha AH., Abo-Elmaaty AM., Abdelgawad FE., Metwally MM., Saber T., Mansour MF. Thymol alleviates imidacloprid-induced testicular toxicity by modulating oxidative stress and expression of steroidogenesis and apoptosis-related genes in adult male rats. Ecotoxicology and Environmental Safety 2021; 221: 112435. https://doi.org/10.1016/j.ecoenv.2021.112435
  49. Sahibzada MUK., Sadiq A., Faidah HS., Khurram M., Amin MU., Haseeb A., Kakar M. Berberine nanoparticles with enhanced in vitro bioavailability: characterization and antimicrobial activity. Drug Design, Development and Therapy 2018; 303-312. https://doi.org/10.2147/DDDT.S156123
  50. Tonietto BD., Laurentino AOM., Costa-Valle MT., Cestonaro LV., Antunes BP., Sates C., dos Santos NG., Dallegrave E., Garcia SC., Leal MB., Arbo MD. Imidacloprid-based commercial pesticide causes behavioral, biochemical, and hematological impairments in Wistar rats. Environmental Toxicology and Pharmacology 2022; 94: 103924. https://doi.org/10.1016/j.etap.2022.103924
  51. Toor HK., Sangha GK., Khera KS. Imidacloprid induced histological and biochemical alterations in liver of female albino rats. Pesticide Biochemistry and Physiology 2013; 105(1): 1-4. https://doi.org/10.1016/j.pestbp.2012.10.001
  52. Uzun F., Kalender Y. Protective effect of vitamins C and E on malathion-induced nephrotoxicity in male rats. Gazi University Journal of Science 2011; 24(2): 193-201.
  53. Uzunhisarcikli M., Apaydin FG., Bas H., Kalender Y. The ameliorative effects of quercetin and curcumin against subacute nephrotoxicity of fipronil induced in Wistar rats. Toxicology Research 2023; 12(3): 493-502. https://doi.org/10.1093/toxres/tfad034
  54. Wang X., Sun J., Xu T., Lei Y., Gao M., Lin H. Resveratrol alleviates imidacloprid‐induced mitochondrial apoptosis, necroptosis, and immune dysfunction in chicken lymphocyte lines by inhibiting the ROS/MAPK signaling pathway. Environmental Toxicology 2024; 39(4): 2052-2063. https://doi.org/10.1002/tox.24097
  55. Xie W., Chen C., Li H., Tu Y., Zhong Y., Lin Z., Cai Z. Imidacloprid-induced lung injury in mice: Activation of the PI3K/AKT/NF-κB signaling pathway via TLR4 receptor engagement. Science of The Total Environment 2024; 931: 172910. https://doi.org/10.1016/j.scitotenv.2024.172910
  56. Xu Z., Xie L., Li H., You J. Sensitivity variations in developmental toxicity of imidacloprid to Zebrafish embryos at different neurodevelopmental stages. Environmental Toxicology and Chemistry 2024; 43(11): 2398-2408. https://doi.org/10.1002/etc.5986
  57. Zouaoui S., Rouabhi R. Lysosomal disruption, mitochondrial impairment, histopathological and oxidative stress in rat's nervous system after exposure to a neonicotinoid (imidacloprid). Environmental Science and Pollution Research 2024; 31(49): 59472-59489. https://doi.org/10.1007/s11356-024-35195-5.