SavedAdd your journal

Journals / Medical Records / 2026 / Sayı: 1

Protective Effect of Olive Leaf Extract on Renal Ischemia/Reperfusion Injury in Rats

Ratlarda Zeytin Yaprağı Ekstresinin Böbrek İskemi/Reperfüzyon Hasarına Karşı Koruyucu Etkisi

Hazen Sarıtaş(AKSARAY UNIVERSITY, SCHOOL OF MEDICINE)
Fatma Görücü Özbek(AFYON KOCATEPE UNIVERSITY, FACULTY OF VETERINARY MEDICINE)
Hasan Hüseyin Demirel(AFYON KOCATEPE UNIVERSITY, FACULTY OF VETERINARY MEDICINE)
Aziz Bülbül(MUGLA SITKI KOCMAN UNIVERSITY, MILAS FACULTY OF VETERINARY MEDICINE, DEPARTMENT OF PRECLINICAL SCIENCES)
Yusuf Koç(AFYON KOCATEPE UNIVERSITY, FACULTY OF VETERINARY MEDICINE)
Murat Kanat(AFYON KOCATEPE UNIVERSITY, FACULTY OF VETERINARY MEDICINE)
Zülfükar Sarıtaş(AFYON KOCATEPE UNIVERSITY, FACULTY OF VETERINARY MEDICINE)
Pages
—
Publication Date
2026-03-24
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This study is the first comprehensive dose-comparative investigation demonstrating that pre-treatment with OLE significantly mitigates renal damage, inflammation, and oxidative stress, suggesting its potential therapeutic application in clinical settings prone to renal ischaemic damage.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Aim: Renal ischaemia/reperfusion (I/R) injury, which is an important contributing factor to acute kidney damage, leading to increased morbidity and mortality.
📊
Key Findings & Data:Material and Methods: A total of 32 male Wistar albino rats were randomly divided into four groups: Sham, Control I/R, I/R+OLE200, and I/R+OLE400.
💡
Conclusion & Impact:This study is the first comprehensive dose-comparative investigation demonstrating that pre-treatment with OLE significantly mitigates renal damage, inflammation, and oxidative stress, suggesting its potential therapeutic application in clinical settings prone to renal ischaemic damage.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Akut Böbrek Hasarı · Zeytin Yaprağı ekstresi · Oksidatif Stres

Abstract

Aim: Renal ischaemia/reperfusion (I/R) injury, which is an important contributing factor to acute kidney damage, leading to increased morbidity and mortality. Olive leaf extract (OLE) possesses antioxidant and anti-inflammatory properties due to its rich polyphenolic content. This study comprehensively investigates the protective effects of OLE obtained from the Memecik variety of olive leaves against renal I/R injury, evaluated by employing two different doses. Material and Methods: A total of 32 male Wistar albino rats were randomly divided into four groups: Sham, Control I/R, I/R+OLE200, and I/R+OLE400. Serum biochemical parameters (urea, creatinine, electrolytes, total antioxidant status [TAS], total oxidant status [TOS], TNF-α, IL-1β, IL-6, and nitric oxide [NO]) and renal tissue histopathology were evaluated. Results: OLE significantly improved serum biochemical parameters, reduced pro-inflammatory cytokines, and enhanced systemic antioxidant capacity, indicated by increased TAS and decreased TOS levels compared to the I/R control group (p<0.05). Histopathologically, OLE groups showed significantly less renal tubular damage and necrosis compared to the control I/R group (p<0.001), with a more pronounced protective effect observed at 400 mg/kg/day. Conclusion: OLE from the Memecik variety exhibits potent protective effects against renal I/R injury. This study is the first comprehensive dose-comparative investigation demonstrating that pre-treatment with OLE significantly mitigates renal damage, inflammation, and oxidative stress, suggesting its potential therapeutic application in clinical settings prone to renal ischaemic damage.

Özet

Giriş: Renal iskemi/reperfüzyon (I/R) hasarı.Akut böbrek hasarına katkıda bulunan, morbidite ve mortaliteyi artıran önemli bir faktördür. Zeytin yaprağı ekstresi (OLE), zengin polifenolik içeriği sayesinde antioksidan ve antiinflamatuar özelliklere sahiptir. Bu çalışmada, Memecik zeytin yaprağından elde edilen OLE'nin renal I/R hasarına karşı koruyucu etkileri kapsamlı bir şekilde incelenmiş ve iki farklı doz kullanılarak değerlendirilmiştir. Yöntemler: Toplam 32 erkek Wistar albino sıçan rastgele dört gruba ayrılmıştır: Sham, Kontrol I/R, I/R+OLE200 ve I/R+OLE400. Serum biyokimyasal parametreleri (üre, kreatinin, elektrolitler, toplam antioksidan durum [TAS], toplam oksidan durum [TOS], TNF-α, IL-1β, IL-6 ve nitrik oksit [NO]) ve böbrek dokusu histopatolojisi değerlendirilmiştir. Sonuçlar: OLE, serum biyokimyasal parametrelerini önemli ölçüde iyileştirdi, proinflamatuar sitokinleri azalttı ve sistemik antioksidan kapasitesini artırdı; bu durum, I/R kontrol grubuna kıyasla TAS'ta artış ve TOS seviyelerinde azalma ile gösterildi (p<0,05). Histopatolojik olarak, OLE grupları kontrol I/R grubuna kıyasla önemli ölçüde daha az renal tübüler hasar ve nekroz gösterdi (p<0,001) ve 400 mg/kg/gün dozda daha belirgin bir koruyucu etki gözlendi. Sonuç: Memecik çeşidinden elde edilen OLE, renal I/R hasarına karşı güçlü koruyucu etkiler göstermektedir. Bu çalışma, OLE ile ön tedavinin böbrek hasarını, inflamasyonu ve oksidatif stresi önemli ölçüde azalttığını gösteren ilk kapsamlı doz karşılaştırmalı araştırmadır ve böbrek iskemik hasarına yatkın klinik ortamlarda potansiyel terapötik uygulamasını düşündürmektedir.

🔖

Cite This Article

Sarıtaş, H., Özbek, F. G., Demirel, H. H., Bülbül, A., Koç, Y., Kanat, M., Sarıtaş, Z. (2026). Protective Effect of Olive Leaf Extract on Renal Ischemia/Reperfusion Injury in Rats. Medical Records, Sayı: 1. https://doi.org/10.37990/medr.1822657

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (41)

  1. Malek M, Nematbakhsh M. Renal ischemia/reperfusion injury; from pathophysiology to treatment. J. Ren. Inj. Prev. 2015;4(2):20.
  2. Wu M-Y, Yiang G-T, Liao W-T et al. Current mechanistic concepts in ischemia and reperfusion injury. Cell Physiol Biochem. 2018;46(4):1650-67.
  3. Shiva N, Sharma N, Kulkarni YA, et al. Renal ischemia/reperfusion injury: An insight on in vitro and in vivo models. Life sci. 2020;256:117860.
  4. Gaut JP, Liapis H. Acute kidney injury pathology and pathophysiology: a retrospective review. Clin. Kidney J. 2021;14(2):526-36.
  5. Pefanis A, Ierino FL, Murphy JM et al. Regulated necrosis in kidney ischemia-reperfusion injury. Kidney int. 2019;96(2):291-301.
  6. Irazabal MV, Torres VE. Reactive oxygen species and redox signaling in chronic kidney disease. Cells. 2020;9(6):1342.
  7. Ishimoto Y, Tanaka T, Yoshida Y et al. Physiological and pathophysiological role of reactive oxygen species and reactive nitrogen species in the kidney. Clin Exp Pharmacol Physiol. 2018;45(11):1097-105.
  8. Kaushal GP, Chandrashekar K, Juncos LA. Molecular interactions between reactive oxygen species and autophagy in kidney disease. Int. J. Mol. Sci. 2019;20(15):3791.
  9. Nath KA, Norby SM. Reactive oxygen species and acute renal failure. Am. J. Med. 2000;109(8):665-78.
  10. Chung AC, Lan HY. Chemokines in renal injury. J Am Soc Nephrol. 2011;22(5):802-9.
  11. Sahna E, Olmez E, Acet A. Effects of physiological and pharmacological concentrations of melatonin on ischemia–reperfusion arrhythmias in rats: can the incidence of sudden cardiac death be reduced? J. Pineal Res. 2002;32(3):194-8.
  12. Kabasakal L, Şehirli Ö, Çetinel Ş et al. Protective effect of aqueous garlic extract against renal ischemia/reperfusion injury in rats. J Med Food. 2005;8(3):319-26.
  13. Lin M, Li L, Li L et al. The protective effect of baicalin against renal ischemia-reperfusion injury through inhibition of inflammation and apoptosis. BMC Complement Altern Med. 2014;14:1-9.
  14. Zhang J, Tang L, Li GS et al. The anti-inflammatory effects of curcumin on renal ischemia-reperfusion injury in rats. Ren. Fail. 2018;40(1):680-6.
  15. El SN, Karakaya S. Olive tree (Olea europaea) leaves: potential beneficial effects on human health. Nutr. Rev. 2009;67(11):632-8.
  16. Borjan D, Leitgeb M, Knez Ž et al. Microbiological and antioxidant activity of phenolic compounds in olive leaf extract. Mol. 2020;25(24):5946.
  17. Moudache M, Colon M, Nerín C et al. Phenolic content and antioxidant activity of olive by-products and antioxidant film containing olive leaf extract. Food Chem. 2016;212:521-7.
  18. Ghomari O, Sounni F, Massaoudi Y et al. Phenolic profile (HPLC-UV) of olive leaves according to extraction procedure and assessment of antibacterial activity. Biotechnol. Rep. 2019;23:e00347.
  19. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic. 1965;16(3):144-58.
  20. Chen H, Xing B, Liu X et al. Ischemic postconditioning inhibits apoptosis after renal ischemia/reperfusion injury in rat. Transpl Int. 2008;21(4):364-71.
  21. Talhaoui N, Gómez-Caravaca AM, León L et al. Fernández-Gutiérrez A, Segura-Carretero A. From olive fruits to olive oil: phenolic compound transfer in six different olive cultivars grown under the same agronomical conditions. Int. J. Mol. Sci. 2016;17(3):337.
  22. Şahin S, Bilgin M. Olive tree (Olea europaea L.) leaf as a waste by‐product of table olive and olive oil industry: a review. J Sci Food Agric. 2018;98(4):1271-9.
  23. Japón-Luján R, de Castro ML. Superheated liquid extraction of oleuropein and related biophenols from olive leaves. J. Chromatogr. A. 2006;1136(2):185-91.
  24. Xynos N, Papaefstathiou G, Gikas E et al. Design optimization study of the extraction of olive leaves performed with pressurized liquid extraction using response surface methodology. Sep. Purif. Technol. 2014;122:323-30.
  25. Brahmi F, Mechri B, Dabbou S et al. The efficacy of phenolics compounds with different polarities as antioxidants from olive leaves depending on seasonal variations. Ind Crops Prod. 2012;38:146-52.
  26. Nasrallah H, Aissa I, Slim C et al. Effect of oleuropein on oxidative stress, inflammation and apoptosis induced by ischemia-reperfusion injury in rat kidney. Life Sci. 2020;255:117833.
  27. Meurer M, Höcherl K. Renal ischemia-reperfusion injury impairs renal calcium, magnesium, and phosphate handling in mice. Pflug. Arch. Eur. J. Physiol. 2019;471:901-14.
  28. Hosseini F, Naseri MG, Badavi M et al. Effect of beta carotene on lipid peroxidation and antioxidant status following renal ischemia/reperfusion injury in rat. Scand J Clin Lab Invest. 2010;70(4):259-63.
  29. Sancaktutar AA, Bodakci MN, Hatipoglu NK et al. The protective effects of pomegranate extracts against renal ischemia-reperfusion injury in male rats. Urol. Ann. 2014;6(1):46-50.
  30. Banda J, Duarte R, Dix-Peek T et al. Biomarkers for Diagnosis and Prediction of Outcomes in Contrast‐Induced Nephropathy. Int. J. Nephrol. 2020;2020(1):8568139.
  31. Gong D-J, Wang L, Yang Y-Y et al. Diabetes aggravates renal ischemia and reperfusion injury in rats by exacerbating oxidative stress, inflammation, and apoptosis. Ren. Fail. 2019;41(1):750-61.
  32. Haq M, Norman J, Saba SR et al. Role of IL-1 in renal ischemic reperfusion injury. J Am Soc Nephrol. 1998;9(4):614-9.
  33. Kelly K. Distant effects of experimental renal ischemia/reperfusion injury. J Am Soc Nephrol. 2003;14(6):1549-58.
  34. Rusai K, Huang H, Sayed N et al. Administration of interleukin‐1 receptor antagonist ameliorates renal ischemia‐reperfusion injury. Transpl. Int. 2008;21(6):572-80.
  35. Patel NS, Chatterjee PK, Di Paola R et al. Endogenous interleukin-6 enhances the renal injury, dysfunction, and inflammation caused by ischemia/reperfusion. J. Pharmacol. Exp. Ther. 2005;312(3):1170-8.
  36. De Vries D, Lindeman J, Tsikas D et al. Early renal ischemia-reperfusion injury in humans is dominated by IL-6 release from the allograft. Am. J. Transplant. 2009;9(7):1574-84.
  37. Kaushal GP, Chandrashekar K, Juncos LA. Molecular interactions between reactive oxygen species and autophagy in kidney disease. Int. J. Mol. Sci. 2019;20(15):3791.
  38. Chung AC, Lan HY. Chemokines in renal injury. J Am Soc Nephrol. 2011;22(5):802-9.
  39. Hosseini F, Naseri MG, Badavi M et al. Effect of beta carotene on lipid peroxidation and antioxidant status following renal ischemia/reperfusion injury in rat. Scand J Clin Lab Invest. 2010;70(4):259-63.
  40. Haq M, Norman J, Saba SR et al. Role of IL-1 in renal ischemic reperfusion injury. J Am Soc Nephrol. 1998;9(4):614-9.
  41. Kelly K. Distant effects of experimental renal ischemia/reperfusion injury. J Am Soc Nephrol. 2003;14(6):1549-58.