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Journals / Journal of the Turkish Chemical Society Section A: Chemistry / 2026 / Volume: 12 Issue: 2

Preparation and in Vitro Adhesive Application of Visible Light-Activated Modified Sodium Alginate

Rumeysa Tutar(İSTANBUL ÜNİVERSİTESİ-CERRAHPAŞA)0000-0002-4743-424X
Pages
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Publication Date
2025-05-31
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In particular, 1/10 mM Ru/SPS showed the greatest effect and created the most statistically significant difference.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Materials that are responsive to visible light have been extensively used in biomedical applications, including tissue engineering.
📊
Key Findings & Data:In this study, different concentrations of Ruthenium (Ru) and sodium persulfate (SPS) photoinitiators (0.2/2, 0.5/5, and 1/10 mM) were prepared.
💡
Conclusion & Impact:In particular, 1/10 mM Ru/SPS showed the greatest effect and created the most statistically significant difference.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:AlgMA · Ru/SPS · Light-activation

Abstract

Materials that are responsive to visible light have been extensively used in biomedical applications, including tissue engineering. Tissue adhesives are among the most important applications of tissue engineering. In this study, different concentrations of Ruthenium (Ru) and sodium persulfate (SPS) photoinitiators (0.2/2, 0.5/5, and 1/10 mM) were prepared. Sodium alginate (Na-alginate) was modified with methacrylate (AlgMA) to render it photoactive. Photoactive materials prepared with different ratios of photoinitiators were physically, chemically, morphologically, and mechanically tested. The results of the different analyses supported each other. Ru/SPS concentrations of 0.2/2, 0.5/5, and 1/10 mM were used to evaluate the hydrogel structures. No physical, chemical, or mechanical differences were observed between the 0.5/5, and 1/10 mM. The in vitro adhesion properties of the hydrogels increased significantly from 0.2/2 mM to 1/10 mM Ru/SPS. As the Ru/SPS concentration was increased, a significant increase in cell viability was observed. In particular, 1/10 mM Ru/SPS showed the greatest effect and created the most statistically significant difference.

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Cite This Article

Tutar, R. (2025). Preparation and in Vitro Adhesive Application of Visible Light-Activated Modified Sodium Alginate. Journal of the Turkish Chemical Society Section A: Chemistry, Volume: 12 Issue: 2. https://doi.org/10.18596/jotcsa.1624684

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  24. Sellimi S, Younes I, Ayed H Ben, Maalej H, Mon-tero V, Rinaudo M, et al. Structural, physicochemi-cal and antioxidant properties of sodium alginate isolated from a Tunisian brown seaweed. Int J Biol Macromol [Internet]. 2015 Jan 1;72:1358–67. Available from:.
  25. Rinaudo M. Main properties and current applica-tions of some polysaccharides as biomaterials. Polym Int [Internet]. 2008 Mar 9;57(3):397–430. Available from:.
  26. Ishikawa K, Miyamoto Y, Takechi M, Toh T, Kon M, Nagayama M, et al. Non-decay type fast-setting calcium phosphate cement: Hydroxyapatite putty containing an increased amount of sodium algina-te. J Biomed Mater Res [Internet]. 1997;36(3):393–9. Available from:.
  27. Szekalska M, Puciłowska A, Szymańska E, Ciosek P, Winnicka K. Alginate: Current use and future perspectives in pharmaceutical and biomedical applications. Int J Polym Sci [Internet]. 2016 Jan 1;2016(1):7697031. Available from:.
  28. Paul UC, Manian AP, Široká B, Duelli H, Bechtold T. Sorption of iron(III)–alginate complexes on cellulose fibres. Cellulose [Internet]. 2013 Oct 6;20(5):2481–90. Available from:.
  29. Dhamecha D, Movsas R, Sano U, Menon JU. Applications of alginate microspheres in therapeu-tics delivery and cell culture: Past, present and future. Int J Pharm [Internet]. 2019 Oct 5;569:118627. Available from:.
  30. Sun F, Guo J, Liu Y, Yu Y. Preparation, characte-rizations and properties of sodium alginate grafted acrylonitrile/polyethylene glycol electrospun nano-fibers. Int J Biol Macromol [Internet]. 2019 Sep 15;137:420–5. Available from:.
  31. Vuocolo T, Haddad R, Edwards GA, Lyons RE, Liyou NE, Werkmeister JA, et al. A highly elastic and adhesive gelatin tissue sealant for gastroin-testinal surgery and colon anastomosis. J Gastro-intest Surg [Internet]. 2012 Apr 1;16(4):744–52. Available from:.
  32. Lim KS, Klotz BJ, Lindberg GCJ, Melchels FPW, Hooper GJ, Malda J, et al. Visible light cross‐linking of gelatin hydrogels offers an enhanced cell mic-roenvironment with improved light penetration depth. Macromol Biosci [Internet]. 2019 Jun 26;19(6):1900098. Available from:.
  33. Tutar R, Ceylan D, Çelebi-Saltik B. Preparation and characterization of conductive and multi-network nanocomposite hydrogels as potential scaffolds for electroactive tissues. New J Chem [Internet]. 2024 Aug 19;48(33):14736–45. Avai-lable from:.
  34. Annabi N, Rana D, Shirzaei Sani E, Portillo-Lara R, Gifford JL, Fares MM, et al. Engineering a spra-yable and elastic hydrogel adhesive with antimic-robial properties for wound healing. Biomaterials [Internet]. 2017 Sep 1;139:229–43. Available from:.
  35. Lim KS, Ramaswamy Y, Roberts JJ, Alves M, Poole‐Warren LA, Martens PJ. Promoting cell survi-val and proliferation in degradable poly(vinyl alco-hol)–tyramine hydrogels. Macromol Biosci [Inter-net]. 2015 Oct 11;15(10):1423–32. Available from:.
  36. Fancy DA, Denison C, Kim K, Xie Y, Holdeman T, Amini F, et al. Scope, limitations and mechanis-tic aspects of the photo-induced cross-linking of proteins by water-soluble metal complexes. Chem Biol [Internet]. 2000 Sep 1;7(9):697–708. Availab-le from:.
  37. Lim KS, Levato R, Costa PF, Castilho MD, Alcala-Orozco CR, van Dorenmalen KMA, et al. Bio-resin for high resolution lithography-based biofabrica-tion of complex cell-laden constructs. Biofabrica-tion [Internet]. 2018 May 11;10(3):034101. Avai-lable from:.
  38. Bouten PJM, Zonjee M, Bender J, Yauw STK, van Goor H, van Hest JCM, et al. The chemistry of tissue adhesive materials. Prog Polym Sci [Inter-net]. 2014 Jul 1;39(7):1375–405. Available from:.
  39. Duarte AP, Coelho JF, Bordado JC, Cidade MT, Gil MH. Surgical adhesives: Systematic review of the main types and development forecast. Prog Polym Sci [Internet]. 2012 Aug 1;37(8):1031–50. Available from:.
  40. Spotnitz WD, Burks S. Hemostats, sealants, and adhesives: components of the surgical tool-box. Transfusion [Internet]. 2008 Jul 8;48(7):1502–16. Available from:.
  41. ASTM F2392-04, 2015, ASTM International, West Conshohocken, PA [Internet]. Available from:.
  42. Tutar R, Yüce-Erarslan E, İzbudak B, Bal-Öztürk A. Photocurable silk fibroin-based tissue sealants with enhanced adhesive properties for the treat-ment of corneal perforations. J Mater Chem B [In-ternet]. 2022 Apr 13;10(15):2912–25. Available from:.
  43. Tavafoghi M, Sheikhi A, Tutar R, Jahangiry J, Baidya A, Haghniaz R, et al. Engineering tough, injectable, naturally derived, bioadhesive compo-site hydrogels. Adv Healthc Mater [Internet]. 2020 May 24;9(10):1901722. Available from:.
  44. Kim H, Jang JH, Han W, Hwang HJ, Jang J, Kim JY, et al. Extracellular matrix-based sticky sealants for scar-free corneal tissue reconstruction. Bioma-terials [Internet]. 2023 Jan 1;292:121941. Availab-le from:.
  45. Murphy CM, Haugh MG, O’Brien FJ. The effect of mean pore size on cell attachment, proliferation and migration in collagen–glycosaminoglycan scaffolds for bone tissue engineering. Biomaterials [Internet]. 2010 Jan 1;31(3):461–6. Available from:.