Journals / Alatarım / 2008 / Cilt: 7 - Sayı: 2

Regeneration of Begonia semperflorens by direct organogenesis

Begonia semperflorens'in direk organogenesis yöntemiyle rejenerasyonu

Journal
Alatarım
Pages
9–13
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The best regeneration and growth were obtained from the medium containing 0.5 BA - 0.5 NAA (67.5%) followed by 0.5 BA - 0.2 NAA (66.7%) and 0.2 BA - 0.5 NAA (65.0%) in BA and NAA combination.

📌 3 Key Takeaways

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Objective & Hypothesis:The plant cell, tissue or organ culture of many ornamental species and their regeneration are essential for providing the material and systems for their genetic manipulation, and this is therefore the first requirement of genetic engineering.
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Key Findings & Data:In this research, MS media containing different concentrations of BA (0.0, 0.2, 0.5, 1.0 mg/l.) with NAA (0.0, 0.2, 0.5 mg/l.) were investigated to optimize regeneration of Begonia semperflorens.
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Conclusion & Impact:The best regeneration and growth were obtained from the medium containing 0.5 BA - 0.5 NAA (67.5%) followed by 0.5 BA - 0.2 NAA (66.7%) and 0.2 BA - 0.5 NAA (65.0%) in BA and NAA combination.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Begonia cucullata · doku kültürü · Begonia

Abstract

The plant cell, tissue or organ culture of many ornamental species and their regeneration are essential for providing the material and systems for their genetic manipulation, and this is therefore the first requirement of genetic engineering. In this research, MS media containing different concentrations of BA (0.0, 0.2, 0.5, 1.0 mg/l.) with NAA (0.0, 0.2, 0.5 mg/l.) were investigated to optimize regeneration of Begonia semperflorens. The best regeneration and growth were obtained from the medium containing 0.5 BA - 0.5 NAA (67.5%) followed by 0.5 BA - 0.2 NAA (66.7%) and 0.2 BA - 0.5 NAA (65.0%) in BA and NAA combination.

Özet

Süs bitkilerinde hücre, doku ve organ kültürü yöntemlerinin kullanılması ve rejenerasyonu, genetik mühendisliği çalışmalarında ilk aşamayı oluşturmakta ve genetik manipulasyonlarda materyallerin temininde kullanılmaktadır. Bu araştırmada Begonia semperflorens’de rejenerasyon optimizasyonunu sağlamak amacıyla farklı BA (0.0, 0.2, 0.5, 1.0 mg/l) ve NAA (0.0, 0.2, 0.5 mg/l) konsantrasyonlarını içeren MS ortamları denenmiştir. En iyi rejenerasyon oranları sırasıyla; 0.5 BA - 0.5 NAA (%67.5), 0.5 BA - 0.2 NAA (%66.7) ve 0.2 BA - 0.5 NAA (%65.0) içeren ortamlardan sağlanmıştır.

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Cite This Article

MENDİ, N. Y. Y., KOCAMAN, E., ÜNEK, C., ELDOĞAN, S., ÇÜRÜK, P., GENCEL, G., SERÇE, S. (2008). Regeneration of Begonia semperflorens by direct organogenesis. Alatarım, Cilt: 7 - Sayı: 2, 9–13. https://ajindex.com/articles/151303

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