SavedAdd your journal

Journals / Journal of Health Sciences and Medicine / 2026 / Volume: 6 Issue: 4

Evaluation and epigenetic impact of B12, vitamin D, folic acid and anemia in Hashimoto's thyroiditis: a clinical and molecular docking study

Evaluation and epigenetic impact of B12, vitamin D, folic acid and anemia in Hashimato's thyroiditis

Elif Sibel Aslan(BİRUNİ ÜNİVERSİTESİ)0000-0002-3081-9004
Savaş Gür0000-0003-1818-4795
Pages
—
Publication Date
2023-07-30
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Furthermore, molecular docking simulations indicated that folic acid could potentially act as a potent inhibitor of human extracellular signal-regulated kinase (ERK2), which has been reported to play a key role in HT.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Aims: Our approach in this study is to investigate the collective effect of various parameters, including vitamin B12 (B12), vitamin D (Vit-D), folic acid, and iron deficiency, on Hashimoto's thyroiditis (HT) disease.
📊
Key Findings & Data:Methods: The study evaluated age, gender, thyroid stimulating hormone (TSH), free-T4 (FT4), free-T3 (FT3), Vit-D levels, as well as autoantibodies against thyroid peroxidase (anti-TPO) and anti-tyroglobulin iron (anti-TG) levels, ferritin, B12, and folic acid parameters in a total of 30 HT patients and 37 non-HT patients.
💡
Conclusion & Impact:Furthermore, molecular docking simulations indicated that folic acid could potentially act as a potent inhibitor of human extracellular signal-regulated kinase (ERK2), which has been reported to play a key role in HT.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Hashimoto · otoimmun disease · vitamin D

Abstract

Aims: Our approach in this study is to investigate the collective effect of various parameters, including vitamin B12 (B12), vitamin D (Vit-D), folic acid, and iron deficiency, on Hashimoto's thyroiditis (HT) disease. This differs from existing literature that has examined these parameters individually. Methods: The study evaluated age, gender, thyroid stimulating hormone (TSH), free-T4 (FT4), free-T3 (FT3), Vit-D levels, as well as autoantibodies against thyroid peroxidase (anti-TPO) and anti-tyroglobulin iron (anti-TG) levels, ferritin, B12, and folic acid parameters in a total of 30 HT patients and 37 non-HT patients. These parameters were assessed by analyzing the patients' routine blood test results using automated analysis methods. Results: A negative correlation was found between the blood, Vit-D level and anti-TG (as the vit-D value increases, the anti-TG decreases) (r=-0.417; p=0.001; p 0.05). However, the study emphasized the critical role of vitamin D in the pathophysiology and treatment of HT. Furthermore, molecular docking simulations indicated that folic acid could potentially act as a potent inhibitor of human extracellular signal-regulated kinase (ERK2), which has been reported to play a key role in HT.

Özet

Aim:This study aimed to evaluate the frequency of B12, vitamin D, folic acid and iron deficiency, which play a role in the pathogenesis and epigenetic effect on HT, compared to individuals without thyroid disease. Materials and Methods:Age, gender, thyroid stimulating hormone (TSH), free-T4 (FT4), free-T3 (FT3), vit-D among 30 HT patients and 37 non-HT patients admitted to the outpatient clinic between January and December 2021, anti-TPO and Anti-Tyroglobulin iron, vit-D, ferritin, B12, folic acid parameters were evaluated by checking the patients routine blood test results taken by autoanalysis between groups. Results:When the vit-D levels of 30 patients with HT and 37 patients without a diagnosis of HT were compared, a difference was found between vit-D levels. A negative correlation was found between the blood, vit-D level and Anti-TG (as the vit-D value increases, the Anti-Tyroglobulin value decreases) (r=-0.417; p=0.001; p 0.05). Once again, the importance of vit-D deficiency in the physiopathology and treatment of HT, the elimination of vit-D deficiency have actually been shown how important in the treatment process of HT. On the other hand, molecular docking simulations revealed that folic acid might be a strong inhibitor of human extracellular signal-regulated kinase (ERK2) which was reported to have a key role in HT.

🔖

Cite This Article

Aslan, E. S., Gür, S. (2023). Evaluation and epigenetic impact of B12, vitamin D, folic acid and anemia in Hashimoto's thyroiditis: a clinical and molecular docking study. Journal of Health Sciences and Medicine, Volume: 6 Issue: 4. https://doi.org/10.32322/jhsm.1243597

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (26)

  1. Ragusa F, Fallahi P, Elia G, et al. Hashimotos’ thyroiditis: epidemiology, pathogenesis, clinic and therapy. Best Practice Res Clin Endocrinol Metab. 2019;33(6):101367.
  2. McLeod DS, Caturegli P, Cooper DS, et al. Variation in rates of autoim- mune thyroid disease by race/ethnicity in US military personnel. JAMA. 2014;311(15):1563–1565.
  3. Rayman MP. Multiple nutritional factors and thyroid disease, with particular reference to autoimmune thyroid disease. Proc Nutr Soc. 2019;78(1):34-44.
  4. Kawicka A, Regulska-Ilow B. Metabolic disorders and nutritional status in autoimmune thyroid diseases. Advances in Hygiene & Experimental Medicine/Postepy Higieny i Medycyny Doswiadczalnej. 2015;69:80-90.
  5. Öztekin, Y, Ayaz A. Otoimmün tiroid hastalıklarında D vitamininin Rolü. İzmir Katip Çelebi Üniversitesi Sağlık Bilimleri Fakültesi Derg. 2015;7(1):179-184.
  6. Wiersinga WM. Clinical relevance of envi- ronmental factors in the pathogenesis of au- toimmune thyroid disease. Endocrinol Metab. 2016;31(2):213-222.
  7. Zaletel K, Gaberscek S. Hashimoto's thyroiditis:from genes to the disease. Curr Genomics. 2011;12(8):576-588.
  8. Aktaş HŞ. Vitamin B12 and Vitamin D levels in patients with autoimmune hypothyroidism and their correlation with anti-thyroid peroxidase antibodies. Med Princ Pract. 2020;29(4);364-370.
  9. Elvira D. The Role of T-Regulatory Expression in Autoimmune Thyroid Disease and its association with Thyroid antibody. J Autoim Disor. 2016;2;2:19.
  10. Krysia R, Szkróbka W, Okopień B. The effect of vitamin D on thyroid autoimmunity in levothyroxine-treated women with Hashimoto’s thyroiditis and normal vitamin D status. Exp Clin Endocrinol Diabetes. 2017;125(4):229-233.
  11. Hu S, Rayman MP. Multiple nutritional factors and the risk of Hashimoto's thyroiditis. Thyroid. 2017;27(5):597-610.
  12. Ashraf TS, De Sanctis V, Yassin M, Wagdy M, & Soliman N. Chronic anemia and thyroid function. Acta Bio Medica: Atenei Parmensis. 2017;88(1):119.
  13. Koç Ş, Güngör K, Güngör ND, Uzunlulu M. Iron deficiency in women with thyroid-specific autoantibodies:a case control study. J Exp Clin Med. 2022;39(1):194-198.
  14. Das P, Dasnayak G, Senapati U. Cytomorphological spectrum of Hashimoto's thyroiditis and its correlatıon with clinical presentation, thyroid function test and antibody level. Int J Sci Res. 2018;7:2277-2282.
  15. Rosen Y, Daich J, Soliman I, Brathwaite E, Shoenfeld Y. Vitamin D and autoimmunity. Scand J Rheumatol. 2016;45(6):439–447.
  16. Kivity S, Agmon-Levin N, Zisappl M, et al. Vitamin D and autoimmune thyroid diseases. Cell Mol Immunol. 2011;8(3):243–247.
  17. Unal AD, Tarcin O, Parildar H, et al. Vitamin D deficiency is related to thyroid antibodies in autoimmune thyroiditis. Cent Eur J Immunol. 2014;39(4):493–497.
  18. Kim D. The role of vitamin D in thyroid diseases. Int J Mol Sci. 2017;18(9):1949.
  19. Mele C, Caputo M, Bisceglia A, et al. Immunomodulatory effects of vitamin D in thyroid diseases. Nutrients. 2020;12(5):1444.
  20. Rubens M, Ramamoorthy V, Saxena P, et al. Association between vitamin D deficiency and hypothyroidism:results from the National Health and Nutrition Examination Survey (NHANES). 2007–2012. BMC Endocr Disord. 2021;21(1):1-9.
  21. Ece A, Sevin F. Exploring QSAR on 4-cyclohexylmethoxypyrimidines as antitumor agents for their inhibitory activity of cdk2. Letters in Drug Design & Discovery. 2010;7:625-631.
  22. Chan K, Frankish N, Zhang T, et al. Bioactive indanes:insight into the bioactivity of indane dimers related to the lead anti-inflammatory molecule PH46A. J Pharm Pharmacol. 2020;72(7):927-937.
  23. Yamali C, Gul HI, Sakarya M.T, et al. Quinazolinone-based benzenesulfonamides with low toxicity and high affinity as monoamine oxidase-A inhibitors:synthesis, biological evaluation and induced-fit docking studies. Bioor Chem. 2022;124:105822.
  24. Luo X, Zheng T, Mao C, et al. Aberrant MRP14 expression in thyroid follicular cells mediates chemokine secretion through the IL-1β/MAPK pathway in Hashimoto’s thyroiditis. Endocr Connect. 2018;7(6):850-858.
  25. Gan XX, Zhong LK, Shen F, et al. Network pharmacology to explore the molecular mechanisms of prunella vulgaris for treating Hashimoto’s thyroiditis. Front Pharmacol. 2021:12.
  26. Beyazit Y, Kocak E, Tanoglu A, Kekilli M. Oxidative stress might play a role in low serum vitamin D associated liver fibrosis among patients with autoimmune hepatitis. Dig Dis Sci. 2015;60(4):1106-1108.