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Journals / Journal of Health Sciences and Medicine / 2026 / Volume: 8 Issue: 4

Turning preferences in the corner turning test and the status of proinflammatory cytokines in the right-left brain hemispheres in the lipopolysaccharide-induced neuroinflammation model

Lipopolisakkarit ile indüklenen nöroinflamasyon modelinde köşe dönme testinde dönüş tercihleri ve sağ-sol beyin hemisferlerinde proinflamatuar sitokinlerin durumu

Hasan Çalışkan(BALIKESIR UNIVERSITY, SCHOOL OF MEDICINE, MEDICINE PR.)0000-0002-3729-1863
Pages
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Publication Date
2025-07-30
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

More studies are needed in neuroinflammation models with the corner-turning test, which examines damage to the hemispheres in neurological experiments.

📌 3 Key Takeaways

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Objective & Hypothesis:Aims: Neuroinflammation is described as an inflammatory response within the nervous system.
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Key Findings & Data:Methods: 12 adults male Wistar albino rats were divided into control (n=6) and neuroinflammation animals (n=6).
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Conclusion & Impact:More studies are needed in neuroinflammation models with the corner-turning test, which examines damage to the hemispheres in neurological experiments.
🔬Design:Lab & Field Experiment
📍Scope:2023 Verileri
⚙️Variables:Anxiety-like behaviors · corner-turning test · neuroinflammation

Abstract

Aims: Neuroinflammation is described as an inflammatory response within the nervous system. The study aimed to investigate the behavior of rats in the corner turning test and the distribution of proinflammatory cytokines between the right/left hemispheres in the neuroinflammation model induced by lipopolysaccharide. Furthermore, locomotor activity, anxiety-like behaviors, and stress-related harderian gland secretion were also analyzed. Methods: 12 adults male Wistar albino rats were divided into control (n=6) and neuroinflammation animals (n=6). The neuroinflammation group received a single dose of 2 mg/kg intraperitoneally LPS, and 1 ml/kg physiological saline was administered to the control group. Six hours after LPS administration, open field test was performed for 5 minutes. The corner rotation test protocol was performed 6 and 24 hours after LPS administration. Subjects were sacrificed 24 hours after LPS administration. TNF-a and IL-1beta were analyzed in the prefrontal cortex, striatum, and serum. All procedures were conducted with the approval of the Balıkesir University Experimental Animals Ethics Committee (Date: 22.11.2023, Decision No: 2023/10-5). Results were analyzed using the students’ T test. Results: In the open field test, locomotor activity decreased (p 0.05). TNF-alpha and IL-1beta significantly increased compared to the control group (p 0.01). Conclusion: In the neuroinflammation group, anxiety-like behaviors and proinflammatory cytokines increased. Stress-related spotting around the eyes and nose increased. According to the corner-turning test, no significant difference was observed. More studies are needed in neuroinflammation models with the corner-turning test, which examines damage to the hemispheres in neurological experiments.

Özet

Amaç: Nöroinflamasyon, sinir sistemi içindeki enflamatuar bir tepki olarak tanımlanır. Bu çalışmada, lipopolisakkarit ile indüklenen nöroinflamasyon modelinde köşe dönme testinde sıçanların davranışları ve sağ/sol hemisferler arasında proinflamatuar sitokinlerin dağılımı incelenmiştir. Ayrıca, lokomotor aktivite, anksiyete benzeri davranışlar ve stresle ilişkili harder bezi salgısı da analiz edilmiştir. Yöntemler: 12 yetişkin erkek Wistar Albino sıçan kontrol (n=6) ve nöroinflamasyon (n=6) gruplarına ayrıldı. Nöroinflamasyon grubuna tek doz 2 mg/kg intraperitoneal LPS, kontrol grubuna ise 1 ml/kg fizyolojik salin uygulandı. LPS uygulamasından 6 saat sonra 5 dakikalık açık alan testi yapıldı. Köşe dönüş testi protokolü LPS uygulamasından 6 ve 24 saat sonra uygulandı. Denekler, LPS uygulamasından 24 saat sonra öldürüldü. TNF-a ve IL-1beta, prefrontal korteks, striatum ve serumda analiz edildi. Tüm prosedürler Balıkesir Üniversitesi Deney Hayvanları Etik Kurulu'nun onayıyla (onay numarası 2023/10-5) gerçekleştirildi. Sonuçlar, öğrencilerin t-testi kullanılarak analiz edildi. Bulgular: Açık alan testinde, lokomotor aktivite azalırken (p 0,05). TNF-alfa ve IL-1beta, kontrol grubuna kıyasla önemli ölçüde arttı (p 0,01). Sonuç: Nöroinflamasyon grubunda anksiyete benzeri davranışlar ve proinflamatuar sitokinler artmıştır. Göz ve burun çevresinde stresle ilişkili lekelenme artmıştır. Köşe dönme testine göre, anlamlı bir fark gözlenmemiştir. Nörolojik deneylerde hemisferlere verilen hasarı inceleyen köşe dönme testi ile nöroinflamasyon modellerinde daha fazla çalışma yapılması gerekmektedir.

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Cite This Article

Çalışkan, H. (2025). Turning preferences in the corner turning test and the status of proinflammatory cytokines in the right-left brain hemispheres in the lipopolysaccharide-induced neuroinflammation model. Journal of Health Sciences and Medicine, Volume: 8 Issue: 4. https://doi.org/10.32322/jhsm.1691744

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