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Journals / Journal of Biotechnology and Strategic Health Research / 2026 / Cilt: 10 Sayı: 2

Morphometric Assessment of Alveolar Bone Loss Following Systemic Darbepoetin Alfa Administration in a Rat Model of Ligature-Induced Periodontitis

Ligatürle İndüklenen Rat Periodontitis Modelinde Sistemik Darbepoetin Alfa Uygulamasının Alveolar Kemik Kaybı Üzerine Etkisinin Morfometrik Değerlendirilmesi

Amin Adil(Special Amin Adil Periodontology and Implantology Clinic, Bursa, Turkey)0000-0001-5667-5080
İzzet Melih Gürkan(ESKİŞEHİR OSMANGAZİ ÜNİVERSİTESİ, DİŞ HEKİMLİĞİ FAKÜLTESİ)0000-0002-7267-4798
Sibel Özdemir(Yunus Emre Devlet Hastanesi Nöroloji Bölümü)0000-0003-4671-0964
Hakan Özdemir(ESKİŞEHİR OSMANGAZİ ÜNİVERSİTESİ)0000-0001-9550-1999
Pages
92–98
Publication Date
2026-09-01
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Further experimental and clinical studies are needed to confirm these findings.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: This experimental study investigated whether systemic administration of darbepoetin alfa could reduce alveolar bone loss in a rat model of ligature-induced periodontitis through morphometric evaluation.
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Key Findings & Data:Materials and Methods: Fifty adult male Sprague–Dawley rats were randomly allocated to five experimental groups (n = 10): Control, Control receiving darbepoetin alfa (0.25 µg/kg/day), Periodontitis, Periodontitis treated with darbepoetin alfa (0.1 µg/kg/day), and Periodontitis treated with darbepoetin alfa (0.25 µg/kg/day).
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Conclusion & Impact:Further experimental and clinical studies are needed to confirm these findings.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Alveolar kemik kaybı · darbepoetin alfa · deneysel periodontitis

Abstract

Aim: This experimental study investigated whether systemic administration of darbepoetin alfa could reduce alveolar bone loss in a rat model of ligature-induced periodontitis through morphometric evaluation. Materials and Methods: Fifty adult male Sprague–Dawley rats were randomly allocated to five experimental groups (n = 10): Control, Control receiving darbepoetin alfa (0.25 µg/kg/day), Periodontitis, Periodontitis treated with darbepoetin alfa (0.1 µg/kg/day), and Periodontitis treated with darbepoetin alfa (0.25 µg/kg/day). Experimental periodontitis was established by placing silk ligatures around the mandibular first molars. Darbepoetin alfa was administered intraperitoneally once daily throughout the 11-day experimental period. Following euthanasia, morphometric assessment of alveolar bone loss was performed by determining the linear distance between the cementoenamel junction and the alveolar bone crest using stereomicroscopic images. Results: Ligature placement resulted in a marked increase in alveolar bone loss compared with the healthy control groups (p 0.05). Conclusion: Systemic administration of darbepoetin alfa significantly reduced alveolar bone loss in a ligature-induced rat model of periodontitis. Both treatment doses demonstrated comparable protective effects. Further experimental and clinical studies are needed to confirm these findings.

Özet

Amaç: Bu deneysel çalışmada, ligatür ile oluşturulan sıçan periodontitis modelinde sistemik darbepoetin alfa uygulamasının alveolar kemik kaybı üzerindeki etkisinin morfometrik yöntemle değerlendirilmesi amaçlandı. Gereç ve Yöntem: Çalışmaya elli erişkin erkek Sprague–Dawley sıçan dahil edildi ve hayvanlar rastgele beş gruba ayrıldı (n=10): Kontrol, Kontrol + darbepoetin alfa (0,25 µg/kg/gün), Periodontitis, Periodontitis + darbepoetin alfa (0,1 µg/kg/gün) ve Periodontitis + darbepoetin alfa (0,25 µg/kg/gün). Deneysel periodontitis, mandibular birinci molar dişlerin çevresine ipek ligatür yerleştirilerek oluşturuldu. Darbepoetin alfa deney süresince 11 gün boyunca intraperitoneal olarak uygulandı. Deney sonunda mandibulalar çıkarıldı ve alveolar kemik kaybı, stereomikroskop altında mine-sement sınırı ile alveolar kemik kreti arasındaki doğrusal mesafenin ölçülmesiyle morfometrik olarak değerlendirildi. İstatistiksel anlamlılık düzeyi p 0,05). Sonuç: Sistemik darbepoetin alfa, ligatür ile oluşturulan deneysel periodontitis modelinde alveolar kemik kaybını anlamlı düzeyde azaltmıştır. Her iki doz benzer koruyucu etki göstermiştir. Bulguların doğrulanması için ileri deneysel ve klinik çalışmalara gereksinim vardır.

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Cite This Article

Adil, A., Gürkan, İ. M., Özdemir, S., Özdemir, H. (2026). Morphometric Assessment of Alveolar Bone Loss Following Systemic Darbepoetin Alfa Administration in a Rat Model of Ligature-Induced Periodontitis. Journal of Biotechnology and Strategic Health Research, Cilt: 10 Sayı: 2, 92–98. https://doi.org/10.34084/bshr.2004241

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