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Journals / International Journal of Traditional and Complementary Medicine Research / 2026 / Volume: 7 Issue: 2

Protective Effects of Walnut (Juglans regia L.) Septum Extract Against Isoniazid-Induced Pulmonary Inflammation in Rats

Özlem Aydın Berktaş(Giresun University, Vocational School of Health Services)0000-0002-7235-4890
Arif Keskin(GIRESUN UNIVERSITY)0000-0002-1634-1091
Shadi Asadabadi(SELCUK UNIVERSITY)0009-0009-5510-9605
Pages
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Publication Date
2026-08-14
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⚡ TL;DR(Too Long; Didn't Read)

Conclusion : Walnut septum methanol extract exhibited significant protective effects against INH-induced pulmonary inflammation likely through its antioxidant properties, suggesting potential therapeutic value in preventing INH-associated pulmonary toxicity.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: Isoniazid (INH) is a first-line antituberculosis drug that can induce oxidative stress and tissue damage.
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Key Findings & Data:Material and Methods: Thirty-two adult male Wistar-Albino rats (250-300 g) were randomly divided into four groups (n=8): Control (saline), INH (400 mg/kg), INH + walnut septum extract 100 mg/kg, and INH + walnut septum extract 250 mg/kg.
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Conclusion & Impact:Conclusion : Walnut septum methanol extract exhibited significant protective effects against INH-induced pulmonary inflammation likely through its antioxidant properties, suggesting potential therapeutic value in preventing INH-associated pulmonary toxicity.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Isoniazid · walnut septum · pulmonary inflammation

Abstract

Aim: Isoniazid (INH) is a first-line antituberculosis drug that can induce oxidative stress and tissue damage. Walnut ( Juglans regia L.) septum extract contains high levels of antioxidant compounds with potential therapeutic applications. This study aimed to investigate the protective effects of walnut septum methanolic extract against isoniazid (INH)-induced pulmonary inflammation in rats. Material and Methods: Thirty-two adult male Wistar-Albino rats (250-300 g) were randomly divided into four groups (n=8): Control (saline), INH (400 mg/kg), INH + walnut septum extract 100 mg/kg, and INH + walnut septum extract 250 mg/kg. All treatments were administered orally for 7 consecutive days. Lung tissues were analyzed for lipid peroxidation (LPO), glutathione (GSH), and catalase (CAT) levels, accompanied by comprehensive histopathological examination. Results: INH administration significantly increased LPO levels and decreased GSH content and CAT activity in lung tissue compared to controls (p<0.05). Walnut septum extract dose-dependently reduced LPO levels and restored GSH content and CAT activity. Histopathologically, INH induced interalveolar cellular infiltration and peribronchial lymphoid tissue hyperplasia. Walnut septum extract, particularly at 250 mg/kg, significantly preserved normal pulmonary architecture and attenuated inflammatory changes. Conclusion : Walnut septum methanol extract exhibited significant protective effects against INH-induced pulmonary inflammation likely through its antioxidant properties, suggesting potential therapeutic value in preventing INH-associated pulmonary toxicity.

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Cite This Article

Berktaş, Ö. A., Keskin, A., Asadabadi, S. (2026). Protective Effects of Walnut (Juglans regia L.) Septum Extract Against Isoniazid-Induced Pulmonary Inflammation in Rats. International Journal of Traditional and Complementary Medicine Research, Volume: 7 Issue: 2. https://doi.org/10.53811/ijtcmr.1851219

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