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Journals / Alatarım / 2009 / Cilt: 8 - Sayı: 2

Genetic characterization of some Citrus rootstocks by using SSR markers

Bazı turunçgil anaçlarının SSR Markırları ile moleküler tanımlanması

Yıldız Aka KAÇAR
TURGUT YEŞİLOĞLU
Bilge YILDIRIM
ÖZHAN ŞİMŞEK
MERAL İNCESU
Müge KAMİLOĞLU
Önder TUZCU
Journal
Alatarım
Pages
8–16
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The genetic relationships between genotypes are discussed.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study genetic characterization of twenty-three Citrus rootstocks has been conducted by using SSR markers.
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Key Findings & Data:Primer pair Ci03C08 did not give clear amplification and reproducible fragments with all genotypes.
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Conclusion & Impact:The genetic relationships between genotypes are discussed.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:PAGE · mikrosatellitler · anaçlar

Abstract

In this study genetic characterization of twenty-three Citrus rootstocks has been conducted by using SSR markers. The ten SSR primer pairs were used to fingerprint the rootstock collection. Primer pair Ci03C08 did not give clear amplification and reproducible fragments with all genotypes. The number of fragments amplified from individual citrus genotypes with each primer pair ranged from one to three. Primer pair CI03D12a was the most informative and polymorphic that produced ten fragment with the genotypes studied ranged from 238 bp to 258 bp. The data were analyzed to calculate genetic similarity values and UPGMA Cluster Analysis was performed to generate a dendrogram. The genetic relationships between genotypes are discussed.

Özet

Bu araştırmada, turunçgillerde anaç olma özelliği olan yirmi üç genotipin genetik yapıları SSR (Simple Sequence Repeat) markırları ile belirlenmiştir. Genotiplerin genetik ilişkilerinin belirlenmesi amacıyla toplam on SSR primer çifti kullanılmıştır. Kullanılan primerlerden Ci03C08 primeri istenilen amplifikasyonu vermediği için değerlendirmeye alınmamıştır. Değerlendirmeye alınan 9 mikrosatellit primer çifti her genotip için 1 ile 3 arasında değişen bantlar vermiştir. CI03D12a primer çifti, proje kapsamında çalışılan genotiplerle toplam 10 bant vererek en yüksek polimorfizm oranını sergilemiştir. Bu primer için elde edilen bantlar 238bp-258bp aralığında dağılmışlardır. Elde edilen genetik veriler ile genetik benzerlikler hesaplanmış ve genetik benzerliklere dayalı dendrogram elde edilmiştir. Araştırma kapsamında kullanılan genotipler arasındaki genetik benzerlikler tartışılmıştır.

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Cite This Article

KAÇAR, Y. A., YEŞİLOĞLU, T., YILDIRIM, B., ŞİMŞEK, Ö., İNCESU, M., KAMİLOĞLU, M., TUZCU, Ö. (2009). Genetic characterization of some Citrus rootstocks by using SSR markers. Alatarım, Cilt: 8 - Sayı: 2, 8–16. https://ajindex.com/articles/149763

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