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Journals / International Journal of Health Services Research and Policy / 2019 / Volume: 4 Issue: 3

IMMUNOHISTOCHEMICAL ANALYSIS OF SFLT-1 AND ADAMTS-8 EXPRESSION IN DIABETIC RAT TESTIS TISSUE

Seval Kaya(DICLE UNIVERSITY, FACULTY OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF HISTOLOGY AND EMBRYOLOGY)0000-0001-6251-6529
Yusuf Nergiz(DICLE UNIVERSITY, FACULTY OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF HISTOLOGY AND EMBRYOLOGY)0000-0002-9988-9385
Fırat Asır(DICLE UNIVERSITY, FACULTY OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF HISTOLOGY AND EMBRYOLOGY)0000-0002-6384-9146
Ebru Gökalp Özkorkmaz(DICLE UNIVERSITY, FACULTY OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF HISTOLOGY AND EMBRYOLOGY)0000-0002-1967-4844
Gamze Erdogan(DICLE UNIVERSITY, FACULTY OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF HISTOLOGY AND EMBRYOLOGY)0000-0002-5854-8517
Engin Deveci(DICLE UNIVERSITY, FACULTY OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF HISTOLOGY AND EMBRYOLOGY)0000-0001-5493-7083
Pages
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Publication Date
2019-12-13
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It is thought that the distribution of ADAMTS-8 may be a determinant protein in the development of extracellular matrix and in damage to testicular tissue of diabetic testis .

📌 3 Key Takeaways

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Objective & Hypothesis:Abstract: The aim of this study was to determine the testicular inflammation and angiogenetic effect of diabetes with ADAMTS-8 and SFlt-1 proteins immunohistochemically.
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Key Findings & Data:Wistar albino male rats (n: 12)were used for the study, Group 1: Control group (n: 6), only 1 ml i.p. saline injection was performed.
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Conclusion & Impact:It is thought that the distribution of ADAMTS-8 may be a determinant protein in the development of extracellular matrix and in damage to testicular tissue of diabetic testis .
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:diabetes · testis · ADAMTS-8

Abstract

Abstract: The aim of this study was to determine the testicular inflammation and angiogenetic effect of diabetes with ADAMTS-8 and SFlt-1 proteins immunohistochemically. Wistar albino male rats (n: 12)were used for the study, Group 1: Control group (n: 6), only 1 ml i.p. saline injection was performed. Group 2: Diabetes group (n: 6) received a single intraperitoneal dose of streptozotocin (STZ) of 60 mg / kg. The glucose value measured above 250 mg / dl was considered as diabetic. Under anesthesia, dissection was performed at the lower part of abdominal clearance and testicular tissue was removed. Testis tissues were fixed in 10% neutral formalin, followed by the routine paraffin protocol and cut with a microtome. Then, primary antibodies (ADAMTS-8, sFlt-1) were applied by immunohistochemistry method and incubated at +4 ˚C overnight. The sections were then examined under a light microscope. Diabetes group showed that Leydig cells in the inter tubular area had vacuolization and capillary dilatations in histopathological examinations. sFlt-1 staining of control group showed positive expression in capillary endothelium between Leydig chambers of the intertubular area. Sflt-1 expression of diabetes group was observed in degenerative spermatic cells and Sertoli cells of the basement membrane facing tubules. The expression of ADAMTS-8in control group was positive in some Leydig cells in the interstitial area of Sertoli cells in seminiferous tubule but, spermatogenetic cells were negative. In the ADAMTS-8 staining of diabetes group, the expression of ADAMTS-8 was increased in the stromal cells and some inflammatory cells in the intertubular space. SFlt-1 plays a crucial role in angiogenesis as well as in diabetic testes and is marked as a precursor for the disruption of vascular structure and blood flow due to degenerative changes. It is thought that the distribution of ADAMTS-8 may be a determinant protein in the development of extracellular matrix and in damage to testicular tissue of diabetic testis .

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Cite This Article

Kaya, S., Nergiz, Y., Asır, F., Özkorkmaz, E. G., Erdogan, G., Deveci, E. (2019). IMMUNOHISTOCHEMICAL ANALYSIS OF SFLT-1 AND ADAMTS-8 EXPRESSION IN DIABETIC RAT TESTIS TISSUE. International Journal of Health Services Research and Policy, Volume: 4 Issue: 3. https://doi.org/10.23884/ijhsrp.2019.4.3.07

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