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Journals / Ege Üniversitesi Diş Hekimliği Fakültesi / 2020 / Cilt: 41 - Sayı: 2

Evaluation of the effect of hyaluronic acid on osteoporotic rat bone healing in cortical bone defects with dentin graft

Osteoporoti̇k sıçanlarda hyaluroni̇k asi̇ti̇n denti̇n grefti̇yle korti̇kal kemi̇k defektleri̇nde kemi̇k i̇yi̇leşmesi̇ne etki̇si̇ni̇n değerlendi̇ri̇lmesi

Uğur MERCAN
Merve ÇAKIR
Deniz Gökçe MERAL
Pages
121–129
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Conclusions: Dentin graft combined with hyaluronic acid enhances bone regeneration in critical-size calvarial defects in osteoporotic rats.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: The aim of this study was to evaluate the effect of hyaluronic acid and dentin graft on bone healing of criticalsized cortical osteoporotic bone defects.
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Key Findings & Data:Methods: 24 adult female Wistar rats underwent bilateral ovariectomy.
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Conclusion & Impact:Conclusions: Dentin graft combined with hyaluronic acid enhances bone regeneration in critical-size calvarial defects in osteoporotic rats.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: The aim of this study was to evaluate the effect of hyaluronic acid and dentin graft on bone healing of criticalsized cortical osteoporotic bone defects. Methods: 24 adult female Wistar rats underwent bilateral ovariectomy. Six weeks later, when the animals were osteoporotic, the rats were divided into three groups: Group D (dentin grafted), Group D-HA (dentin grafted with hyaluronic acid) and Group C (control). A 5-mm diameter critical-size defect was created in the calvarium of each animal. In Group D; the defect was filled by dentin graft, in group D-HA; the defect was filled by dentin graft and hyaluronic acid, in group C; the control group was left empty. All animals were euthanized at 28th day. New bone formation at each defect site was evaluated both histologically and immunohistochemically according to hematoxylin and eosin, BMP2, TGFβ, and osteopontin levels. Results: Histologically, group D and group D-HA exhibited significantly more new bone formation than group C. Immunohistochemically; The TGFβ and osteopontin levels were significantly higher in Group D-HA. The BMP2 levels did not differ significantly between the groups D and group D-HA. Conclusions: Dentin graft combined with hyaluronic acid enhances bone regeneration in critical-size calvarial defects in osteoporotic rats.

Özet

Amaç: Bu çalışmanın amacı, osteoporotik sıçanlarda kortikal kemik defektlerinde hyaluronik asit ve dentin greftinin kemik iyileşmesi üzerindeki etkisini değerlendirmektir. Yöntem: Çalışmada 24 adet erişkin dişi sıçan kullanılmıştır. Sıçanlara bilateral ovarektomi yapılmış, altı hafta sonunda osteoporotik olarak kabul edilen sıçanlar, üç gruba ayrılmıştır. Grup D, Grup D-HA ve Grup C. Her hayvanın kalvarium’unda 5 mm çapında kritik boyutta bir defekt oluşturulmuştur. Defektler D grubunda; dentin grefti ile, D-HA grubunda; dentin grefti ve hyaluronik asit ile doldurulmuş, kontrol grubunda (C grubu) ise boş bırakılmıştır. Bütün hayvanlar 28. günde sakrifiye edilmiştir. Her defekt bölgesinde yeni kemik oluşumu; hematoksilen ve eozin, BMP2, TGFβ ve osteopontin seviyelerine göre hem histolojik hem de immünohistokimyasal olarak değerlendirilmiştir. Bulgular: Histolojik olarak grup D ve grup D-HA, grup C’den anlamlı olarak daha fazla yeni kemik oluşumu göstermiştir. İmmünhistokimyasal olarak; TGFβ ve osteopontin seviyeleri Grup D-HA’da anlamlı olarak yüksek bulunmuştur. BMP2 seviyeleri D grubu ve D-HA grubu arasında anlamlı farklılık göstermemiştir. Sonuç: Dentin grefti ve hyaluronik asitle birlikte dentin greftinin osteoporotik kemiklerde yeni kemik oluşumunu hızlandırdığı gözlenmiştir.

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Cite This Article

MERCAN, U., ÇAKIR, M., MERAL, D. G. (2020). Evaluation of the effect of hyaluronic acid on osteoporotic rat bone healing in cortical bone defects with dentin graft. Ege Üniversitesi Diş Hekimliği Fakültesi, Cilt: 41 - Sayı: 2, 121–129. https://ajindex.com/articles/149181

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References (14)

  1. Tanaka M, Toyooka E, Kohno S, Ozawa H, Ejiri S. Long-term changes in trabecular structure of aged rat alveolar bone after ovariectomy. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2003;95(4):495-502
  2. McGrath C, Bedi R. The importance of oral health to older people’s quality of life. Gerodontology 1999;16(1):59-63
  3. Durao SF, Gomes PS, Silva-Marques JM, et al. Bone regeneration in osteoporotic conditions: healing of subcritical-size calvarial defects in the ovariectomized rat. Int J Oral Maxillofac Implants 2012;27(6):1400- 8
  4. Birkenfeld L, Yemini M, Kase NG, Birkenfeld A. Menopause-related oral alveolar bone resorption: a review of relatively unexplored consequences of estrogen deficiency. Menopause 1999;6(2):129-33
  5. Hildebolt CF. Osteoporosis and oral bone loss. Dentomaxillofac Radiol 1997;26(1):3-15
  6. Kim SY, Kim SG, Lim SC, Bae CS. Effects on bone formation in ovariectomized rats after implantation of tooth ash and plaster of Paris mixture. J Oral Maxillofac Surg 2004;62(7):852-7
  7. Thompson DD, Simmons HA, Pirie CM, Ke HZ. FDA Guidelines and animal models for osteoporosis. Bone 1995;17(4 Suppl):125S-33S
  8. Cakir M, Karaca IR, Firat A, Kaymaz F, Bozkaya S. Experimental evaluation of the effects of Ankaferd Blood Stopper and collagenated heterologous bone graft on bone healing in sinus floor augmentation. Int J Oral Maxillofac Implants 2015;30(2):279-85
  9. Kim YK, Lee J, Um IW, et al. Tooth-derived bone graft material. J Korean Assoc Oral Maxillofac Surg 2013;39(3):103-11. 10. de Brito Bezerra B, Mendes Brazao MA, de Campos ML, et al. Association of hyaluronic acid with a collagen scaffold may improve bone healing in critical-size bone defects. Clin Oral Implants Res 2012;23(8):938-42
  10. Patterson J, Siew R, Herring SW, et al. Hyaluronic acid hydrogels with controlled degradation properties for oriented bone regeneration. Biomaterials 2010;31(26):6772-81
  11. Tatli U, Ustun Y, Kurkcu M, et al. Effects of zoledronic acid on healing of mandibular fractures: an experimental study in rabbits. J Oral Maxillofac Surg 2011;69(6):1726-35
  12. Johnston BD, Ward WE. The ovariectomized rat as a model for studying alveolar bone loss in postmenopausal women. Biomed Res Int 2015;2015:635023
  13. Luize DS, Bosco AF, Bonfante S, de Almeida JM. Influence of ovariectomy on healing of autogenous bone block grafts in the mandible: a histomorphometric study in an aged rat model. Int J Oral Maxillofac Implants 2008;23(2):207-14
  14. Boyd SK, Davison P