Journals / Erciyes Tarım ve Hayvan Bilimleri Dergisi / 2026 / Volume: 9

Determination of Some Spiny Plants Feed Value by In Vitro Gas Production Technique

Yazan S. M. Izzeddin(ATATÜRK ÜNİVERSİTESİ)0009-0001-3289-9562
Adem Kaya(ATATÜRK ÜNİVERSİTESİ)0000-0002-7726-6865
Pages
—
Publication Date
2026-05-04
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a result, considering the nutrient composition and feed value parameters determined by in vitro techniques, it was concluded that globe thistle and milk thistle can be used as alternative roughage sources in ruminant feeding during periods when the availability of conventional roughage is insufficient, reduced, or limited.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This aim of study is determine the nutritive value and potential use as alternative roughage sources in ruminant nutrition of Yellow Star-thistle (Centaurea solstitialis), Scotch thistle (Onopordum acanthium), Milk thistle (Silybum marianum), and Globe thistle (Echinops ritro) naturally growing in the rangelands of Erzurum.
📊
Key Findings & Data:Rumen fluid used in the in vitro gas production technique was obtained post-slaughter from a 5-year-old Simmental bull with an approximate body weight of 550 kg at a private abattoir.
💡
Conclusion & Impact:As a result, considering the nutrient composition and feed value parameters determined by in vitro techniques, it was concluded that globe thistle and milk thistle can be used as alternative roughage sources in ruminant feeding during periods when the availability of conventional roughage is insufficient, reduced, or limited.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Spiny plants · in vitro gas production · roughage

Abstract

This aim of study is determine the nutritive value and potential use as alternative roughage sources in ruminant nutrition of Yellow Star-thistle (Centaurea solstitialis), Scotch thistle (Onopordum acanthium), Milk thistle (Silybum marianum), and Globe thistle (Echinops ritro) naturally growing in the rangelands of Erzurum. Rumen fluid used in the in vitro gas production technique was obtained post-slaughter from a 5-year-old Simmental bull with an approximate body weight of 550 kg at a private abattoir. The mean values of the nutrient composition of the spiny plants differed significantly among species. The crude protein (CP) content was lowest in globe thistle, whereas the highest average crude fiber (CF) values were recorded for globe thistle and milk thistle. The 24-h in vitro gas production (GP) and methane production (MP), metabolizable energy (ME), net energy for lactation (NEL), and organic matter digestibility (OMD) values of the spiny plants ranged from 34.82 to 40.67 mL/200 mg DM, 12.94 to 13.30%, 7.74 to 8.47 MJ/kg DM, 4.42 to 4.94 MJ/kg DM, and 57.01 to 64.50%, respectively. The highest GP, ME, and NEL values were observed in globe thistle. The relative feed quality (RFQ) indices of the thorny plants were calculated to range between 84.91 and 128.42, with Deve Dikeni showing the highest feed quality. As a result, considering the nutrient composition and feed value parameters determined by in vitro techniques, it was concluded that globe thistle and milk thistle can be used as alternative roughage sources in ruminant feeding during periods when the availability of conventional roughage is insufficient, reduced, or limited.

Özet

This aim of study is determine the nutritive value and potential use as alternative roughage sources in ruminant nutrition of Yellow Star-thistle (Centaurea solstitialis), Scotch thistle (Onopordum acanthium), Milk thistle (Silybum marianum), and Globe thistle (Echinops ritro) naturally growing in the rangelands of Erzurum. Rumen fluid used in the in vitro gas production technique was obtained post-slaughter from a 5-year-old Simmental bull with an approximate body weight of 550 kg at a private abattoir. The mean values of the nutrient composition of the spiny plants differed significantly among species. The crude protein (CP) content was lowest in globe thistle, whereas the highest average crude fiber (CF) values were recorded for globe thistle and milk thistle. The 24-h in vitro gas production (GP) and methane production (MP), metabolizable energy (ME), net energy for lactation (NEL), and organic matter digestibility (OMD) values of the spiny plants ranged from 34.82 to 40.67 mL/200 mg DM, 12.94 to 13.30%, 7.74 to 8.47 MJ/kg DM, 4.42 to 4.94 MJ/kg DM, and 57.01 to 64.50%, respectively. The highest GP, ME, and NEL values were observed in globe thistle. The relative feed quality (RFQ) indices of the thorny plants were calculated to range between 84.91 and 128.42, with Deve Dikeni showing the highest feed quality. As a result, considering the nutrient composition and feed value parameters determined by in vitro techniques, it was concluded that globe thistle and milk thistle can be used as alternative roughage sources in ruminant feeding during periods when the availability of conventional roughage is insufficient, reduced, or limited.

🔖

Cite This Article

Izzeddin, Y. S. M., Kaya, A. (2026). Determination of Some Spiny Plants Feed Value by In Vitro Gas Production Technique. Erciyes Tarım ve Hayvan Bilimleri Dergisi, Volume: 9. https://doi.org/10.55257/ethabd.1868875

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (15)

  1. ANKOM Technology. (2005). DaisyII Incubator Operator’s Manual. ANKOM Technology Corp., Macedon, NY, USA. A.O.A.C., 1990. Official Methods of Analysis. 15th ed. Association of Official Analytical Chemists, Washington, DC. US.
  2. Bilal, Y., Kamalak, A. (2025). Determination the physical structures and potential nutritive value of ration used in beef production. Journal of Erciyes Agriculture and Animal Science. Journal of Erciyes Agriculture and Animal Science, 8(2):59-71.
  3. Blümmel, M., Lebzien, P. (2001). Predicting ruminal microbial efficiencies of dairy rations by in vitro techniques. Livestock production science, 68(2-3): 107-117.
  4. Blümmel, M., Steingaβ, H., Becker, K. (1997). The relationship between in vitro gas production, in vitro microbial biomass yield and 15N incorporation and its implications for the prediction of voluntary feed intake of roughages. British Journal of Nutrition, 77(6): 911-921.
  5. Canbolat, Ö., Karaman, Ş., 2009. Bazı Baklagil Kaba Yemlerinin in Vitro Gaz Üretimi, Organik Madde Sindirilebilirliği, Nispi Yem Değeri ve Metabolik Enerji İçeriklerinin Karşılaştırılması. Journal of Agricultural Sciences,, 15 (2), 188-195. Ankara.
  6. Ceylan, E., Kamalak, A. (2019). Farklı hasat zamanının pamuk dikeninin (Onopordum acanthium) kimyasal kompozisyonu, in vitro gaz ve metan üretimi üzerine etkisi. ADÜ Ziraat Derg, 16(1):13-18.
  7. Çilo, Ş., Kaya, H. (2025). Elma posasına farklı oranlarda fiğ tohumu ilavesinin silaj kalitesi, yem değeri ve rumen fermantasyon parametreleri üzerine etkisi. MEMBA Su Bilimleri Dergisi, 11(1): 50-61.
  8. DiTomaso, J. M. (2000). Invasive weeds in rangelands: Species, impacts, and management. Weed Science, 48(2): 255-265.
  9. Elfaki, M.A. (2024). Domates hasat artıklarından yapılan silaj ve peletlerin yem değerinin in vitro yöntemlerle belirlenmesi. Doktora Tezi, Lisansüstü Eğitim Enstitüsü, Ondokuz Mayıs Üniversitesi, Samsun. Türkiye.
  10. Gürsoy, E. ve Macit, M., 2017. Comparison of relative feed values of some grasses grown in the grassland and meadow of Erzurum Province. Yuzuncu Yıl University Journal of Agricultural Sciences, 27(3): 309-317.
  11. Hageman, J. H., Fowler, J. L., Suzukida, M., Salas, V. Lecaptain, R. (1988). Analysis of Russian thistle (Salsola species) selections for factors affecting forage nutritional value. Journal of Range Management, 41(2): 155-158.
  12. Kamalak, A., Canbolat, O., Gurbuz, Y., Erol, A., Ozay, O. (2005). Effect of maturity stage on chemical composition, in vitro and in situ dry matter degradation of tumbleweed hay (Gundelia tournefortii L). Small Ruminant Research 58: 149-156.
  13. Karabulut, A., Canbolat, Ö., Kalkan, H., Gürbüzol, F., Sucu, E., Filya, İ., 2007. Comparison of in vitro gas production, metabolizable energy, organic matter digestibility and microbial protein production of some legume hays. Asian-Agust. J. Anim. Sci. 20 (4); 517-522.
  14. Kaya A., Kaya A., Kaya H., Macit M., Palangi V., 2024. Usability of gazelle form beech tree (Fagus Orientalis L.) leaves as an alternative roughage source in dairy cattle: using in vitro gas production method. Journal of the Hellenic Veterinary Medical Society, 75(4): 8325-8334.
  15. Kaya A., Kaya H., Macit M. (2020). Determination in vitro gas production and methane emission values of wheat